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  4. KRT20 Recombinant Monoclonal Antibody

KRT20 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA176322A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA176322A0HU diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.61% DAB.
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA176322A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • Uniprot No.：
    P35900
  • 基因名：
  • 别名：
    Keratin, type I cytoskeletal 20 (Cytokeratin-20) (CK-20) (Keratin-20) (K20) (Protein IT), KRT20
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human KRT20
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    17A11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 基因功能参考文献：
    1. Carcinoid-like/labyrinthine pattern of cell arrangement in vimentin/cytokeratin 20 expressing sebaceous neoplasms may represent a morphological phenotype of sebaceous mantles. PMID: 28027080
    2. In our cohort of specimens with equivocal urothelial atypia, very few patients without a prior diagnosis of bladder cancer progressed to diagnostic cancer (1 of 22), suggesting that CK20 and p53 staining results should be interpreted with caution in de novo atypia. PMID: 28967804
    3. results demonstrate the diagnostic value of urinary cytology and confirm CK 20 as an adjunct marker for the diagnosis of urothelial carcinoma. PMID: 28195264
    4. findings of our study imply that sequential staining of CK20 and E-cadherin prevents false-positive classification of BCC. Furthermore, our study demonstrated that p40 exhibits the same staining pattern in BCC, TE, and TB PMID: 26180934
    5. In stomach adenocarcinomas, CDH17 positively stained 64.0% (112 of 175) of tissues, compared to CK20 and CDX2, where staining was observed in only 24.6% (43 of 175) and 46.9% (82 of 175), respectively. PMID: 28029907
    6. results demonstrated upregulation of fibroblast growth factor receptor 1 and CK20 expressions in cancer bladder tissues PMID: 27259667
    7. K20 expression was detected in 6 of 75 patients with triple-negative carcinoma PMID: 26670478
    8. Circulating tumor cell CK20 and survivin expression may effectively predict overall survival in metastic colorectal cancer patients receiving chemotherapy. PMID: 26227487
    9. During postoperative follow-up, serum negative CK20 patients had significantly higher 3-year survival rates than serum positive CK20 patients. PMID: 26125781
    10. Patients with any EGFR, CK19, CK20 or survinin positivity in their peripheral blood obtain less benefit from radiotherapy. PMID: 25854400
    11. Case Report: Merkel cell carcinma that initially demonstrated cytokeratin 20 positivity but lost expression in subsequent brain metastases. PMID: 24901475
    12. Our data show that inclusion of CK20 as a biomarker efficiently improves the detection of CTCs in colorectal cancer patients PMID: 25528628
    13. The present study confirmed that CK14, but not CK20 or CK7, is expressed in urothelial carcinoma with squamous differentiation and squamous cell carcinoma of the urinary bladder. PMID: 25643514
    14. Immunostaining of CDX2 and CK20 provides beneficial information if considering whether to perform an endoscopic papillectomy (EP). PMID: 25081540
    15. High cytokeratin 20 expression is associated with invasive histological phenotype in poorly differentiated colorectal adenocarcinoma. PMID: 24403457
    16. Its association with clinical stage indicates that CK20 might have prognostic value as a marker for detection of circulating CRC cells. PMID: 23558939
    17. The CDX2/CK20 phenotype defines a distinct subgroup of colorectal cancers with microsatellite instability and poor differentiation PMID: 24025523
    18. Data show that lower survival (OS) and disease-free survival (DFS) rates were significantly associated with guanylate cyclase C (GCC) and CK20 mRNA levels. PMID: 23150200
    19. Overexpression of CK20 is associated with early-onset colorectal cancer. PMID: 23322277
    20. High cytokeratin 20 mrna expression is associated with lymphatic metastasis in colon cancer. PMID: 22752373
    21. Pouch/peripouch and UC-associated adenocarcinoma had a comparable positive rate for CK7, CK20, and CDX2 by immunohistochemistry. PMID: 22895272
    22. HER2 and CK20 were seen exclusively in the mucinogenic proliferations in congenital pulmonary airway malformations. PMID: 22348416
    23. CEA mRNA, CK20 mRNA, and serum CEA than patients at stages I-III. PMID: 22414974
    24. Results suggest that CK20 mRNA with other biomarkers in the peripheral blood of breast cancer patients may be useful to monitor the presence of disseminated tumor cells in the blood circulation and to predict the prognosis of breast cancer. PMID: 22677992
    25. Both the CK7-/CK20+ phenotype and expression of the antibody CDX2 are highly specific and sensitive markers of colorectal origin. PMID: 22268990
    26. This is the first reported study of the relationship between CK20/CK7 immunophenotype, BRAF mutations and microsatellite status in colorectal carcinomas PMID: 22361037
    27. positivity rates of CK19 and CK20 in transverse mesocolon were 48.6 and 61.2%, respectively and increased with the depth of tumor invasion PMID: 21938557
    28. Keratin 20 expression is common in colorectal carcinoma. PMID: 22237712
    29. The expressions of CK7 and CK20 in nasal polyps were analyzed. PMID: 22119824
    30. Cytokeratin 20-positive hepatocellular carcinoma PMID: 22073364
    31. CK20 and VEGF expressions in peripheral blood of colorectal carcinoma patients are promising molecular markers for disease progression and metastasis. PMID: 20395351
    32. Merkel cell carcinoma admixed with squamous cell carcinoma can be cytokeratin 20-negative and thyroid transcription factor-1-positive [case report] PMID: 21775107
    33. Our results along with the data from the literature indicate that CK7/CK20 expression may be of clinical significance. PMID: 21574103
    34. SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas. PMID: 21677534
    35. A considerable number of colorectal carcinomas showed no immunoreactivity to CK20. PMID: 21282015
    36. The immunohistochemical subtypes based on CK20and MUC1 expression correlated with ampullary tumor progression. PMID: 21106111
    37. Case Report: CK7+/CK20- Merkel cell carcinoma presenting as inguinal subcutaneous nodules with subsequent epidermotropic metastasis. PMID: 20574624
    38. MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer. PMID: 19914229
    39. CK20 expression is associated with the progression of breast cancer. PMID: 19664394
    40. The expression level of CK20 mRNA in the peripheral blood in patients with gastric cancer declines after postoperative adjuvant chemotherapy. PMID: 19145500
    41. CK20 expression modified in H. pylori chronic gastritis PMID: 11642721
    42. expression in lymph nodes correlates with poor prognosis in colorectal cancer PMID: 11844829
    43. expression pattern is unique to Barrett's esophagus PMID: 11857318
    44. Changing pattern of cytokeratin 7 and 20 expression from normal epithelium to intestinal metaplasia of the gastric mucosa and gastroesophageal junction PMID: 11962749
    45. The detection of cancer metastasis in the lymph nodes in colon carcinoma is almost doubled (21.9% vs 11.1%) by CK-20 mRNA PMID: 12515621
    46. CK 20 mRNA identification by RT-PCR is reliable and may be useful for early diagnosis in peritoneal dissemination of colon cancer PMID: 12636102
    47. It is possible to apply a simple and reliable method for the detection of circulating tumor cells based on cytokeratin-20 and prostate stem cell antigen RT-PCR assays in gastrointestinal cancers. PMID: 12894563
    48. Down regulation of cytokeratin 20 is associated with transitional and squamous cell carcinoma of the bladder PMID: 12954496
    49. The combined expression of CK7 and CK20 has a low specificity in the distinction between esophageal and cardiac (stomach) adenocarcinomas. PMID: 14631371
    50. Alteration of CK7 and CK20 expression profile that occurs early in small intestinal tumorigenesis. PMID: 15371952

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of t
  • 数据库链接：

    HGNC: 20412

    OMIM: 608218

    KEGG: hsa:54474

    STRING: 9606.ENSP00000167588

    UniGene: Hs.84905