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  4. KAT8 Recombinant Monoclonal Antibody

KAT8 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA694283A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA694283A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HepG2 cell with CSB-RA694283A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 587-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The KAT8 recombinant monoclonal antibody was created using genetic engineering methods to clone and express the gene responsible for producing the KAT8 monoclonal antibody. To generate the KAT8 monoclonal antibody, a synthesized peptide from human KAT8 protein was used as the immunogen. The obtained KAT8 recombinant monoclonal antibody was purified using affinity chromatography to ensure its high purity and specificity for the KAT8 protein. It has been extensively validated for use in human samples, and three applications, including ELISA, IHC, and IF, have been conducted to verify the quality and specificity of the KAT8 recombinant monoclonal antibody.

    KAT8, also known as MYST1, is a histone acetyltransferase (HAT) protein that functions as a transcriptional coactivator. It is involved in the regulation of various cellular processes, such as cell cycle progression, DNA damage response, apoptosis, and development. KAT8 specifically acetylates lysine 16 on histone H4, which is associated with gene activation and chromatin remodeling. Additionally, KAT8 has been implicated in the regulation of gene expression through interactions with transcription factors and other chromatin-modifying enzymes. Dysfunction of KAT8 has been linked to various diseases, including cancer and neurodegenerative disorders.

  • Uniprot No.：
    Q9H7Z6
  • 基因名：
    KAT8
  • 别名：
    Histone acetyltransferase KAT8 (EC 2.3.1.48) (Lysine acetyltransferase 8) (MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1) (MYST-1) (hMOF), KAT8, MOF MYST1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KAT8
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7E11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.
  • 基因功能参考文献：
    1. Novel importin alpha1-specific nuclear localization signals were identified in the N-terminal of MOF. PMID: 28991411
    2. Data describe a trans-histone modification pathway involving PKN1/histone H3 threonine 11 phosphorylation followed by WDR5/MLL histone methyltransferase and KAT8/histone acetyltransferase recruitment to effect androgen-dependent gene activation and prostate cancer cell proliferation. PMID: 27268279
    3. These findings provide insight into the regulation of LSD1 and Epithelial-to-Mesenchymal Transition (EMT) and identify MOF as a critical suppressor of EMT and tumor progression. PMID: 27292636
    4. This work identifies MOF as a key regulator of cellular stress response in glomerular podocytes. PMID: 26387537
    5. recent results indicate MOF is an upstream regulator of the ATM (ataxia-telangiectasia mutated) protein, the loss of which is responsible for ataxia telangiectasia (AT). ATM is a key regulatory kinase that interacts with and phosphorylates multiple substrates that influence critical, cell-cycle control and DNA damage repair pathways in addition to other pathways. PMID: 27038808
    6. Histone acetyltransferase activity of MOF is required to sustain MLL-AF9 leukemia and may be important for multiple AML subtypes. PMID: 28202522
    7. these studies point to the critical and specific role of hMOF Lys-274 autoacetylation in hMOF stability and cognate substrate acetylation and argues that binding of Ac-CoA to hMOF likely drives Lys-274 autoacetylation for subsequent cognate substrate acetylation. PMID: 27382063
    8. MOF is a dual-transcriptional regulator of nuclear and mitochondrial genomes connecting epigenetics and metabolism. PMID: 27768893
    9. MOF is highly enriched in induced pluripotent stem cells (iPSCs), and MOF expression is upregulated during the reprogramming process. The ectopic expression of MOF promotes reprogramming. MOF affects Wdr5 and endogenous Oct4 expression. PMID: 26091365
    10. Along with the PHF20/MOF complex, G9a links the crosstalk between ERalpha methylation and histone acetylation that governs the epigenetic regulation of hormonal gene expression. PMID: 26960573
    11. Data found downregulation of hMOF in gastric cancer cells and tissues. Declined hMOF expression, but not high level of HDAC4, may account for global histone H4K16ac suggesting that loss of hMOF expression may be involved in gastric cancer progression. PMID: 25873202
    12. EZH2 (enhancer of zeste homolog 2) was up-regulated in human oral tongue squamous cell carcinoma tissues and its level positively correlated with level of hMOF. PMID: 26032517
    13. Mutant MOF-T392A expression abrogates DSB repair in S/G2 phase cells. MOF-T392A has delayed 53BP1 dissociation and decreased DNA association. PMID: 24953651
    14. Results show the expression of hMOF mRNA and protein was significantly downregulated in ovarian epithelial cancer tissues, and patients with high hMOF levels showed improved survival as compared to those with low hMOF levels. PMID: 25483274
    15. The histone acetyltransferase hMOF suppresses hepatocellular carcinoma growth by targeting the expression of SIRT6. PMID: 25181338
    16. Functional interactions of MYST1 with androgen receptor and NF-KB are critical for prostate cancer progression. PMID: 24702180
    17. hMOF was overexpressed in human non-small cell lung cancer and was a predictor of poor survival. PMID: 24571482
    18. low expression of hMOF was strongly correlated with tumor differentiation and survival of patients with gastric cancer. While in patients with renal cell carcinoma, downregulation of hMOF was connected to ccRCC and tissues with T1 tumor status. PMID: 24452485
    19. review of regulation and function PMID: 24452550
    20. MOF mediates Notch signaling by manipulating Histone H4 acetylation. PMID: 24898892
    21. MOF expression was down-regulated in failing hearts at protein and mRNA levels. PMID: 24802406
    22. The hMOF mediated S phase entry by regulating H4K16ac in the Skp2 promoter region in NSCLC cells. PMID: 23628702
    23. Our results demonstrate an important role of KAT8 in cancer PMID: 23638218
    24. induction of autophagy is coupled to reduction of histone H4 lysine 16 acetylation through downregulation of the histone acetyltransferase hMOF; and this histone modification regulates the outcome of autophagy PMID: 23863932
    25. new insights into the mechanism and function of MYST HAT autoacetylation. PMID: 22918831
    26. uncover novel pathways in which SIRT1 dynamically interacts with and regulates hMOF and TIP60 through deacetylation and provide additional mechanistic insights by which SIRT1 regulates DNA damage response PMID: 22586264
    27. MYST protein acetyltransferase activity requires active site lysine autoacetylation. PMID: 22020126
    28. RNAi-mediated silencing of MOF reduced both gene activation and tumor suppression by FOXP3, while both somatic mutations in clinical cancer samples and targeted mutation of FOXP3 in mouse prostate epithelial cells disrupted nuclear localization of MOF. PMID: 22152480
    29. hMOF is autoacetylated in vitro and in vivo, and SIRT1, the deacetylase for H4K16Ac, is responsible for deacetylating acetylated hMOF. PMID: 21502975
    30. hMOF employs a novel regulatory mechanism of acetyltransferase activities PMID: 21691301
    31. data indicate that H4K20me3 invokes gene repression by antagonizing hMOF-mediated H4K16Ac PMID: 21321083
    32. MOF activity was associated with general chromatin upon DNA damage and colocalized with the synaptonemal complex in male meiocytes. PMID: 20479123
    33. Although MSL-associated MOF acetylates nucleosomal histone H4 almost exclusively on lysine 16, NSL-associated MOF exhibits a relaxed specificity and also acetylates nucleosomal histone H4 on lysines 5 and 8. PMID: 20018852
    34. These results suggest that hMOF influences the function of ATM. PMID: 15923642
    35. hMOF has a role in DNA damage response during cell cycle progression. PMID: 16024812
    36. hMOF is an important component of many cellular processes and plays role the in cell malignant transformation. PMID: 17694080
    37. MOF is an essential factor for embryogenesis and oncogenesis PMID: 17967868
    38. downregulation of hMOF protein expression was associated with lower survival rates identifying hMOF as an independent prognostic marker for clinical outcome in univariate as well as multivariate analyses PMID: 18058815
    39. Data show that MOF acetylates TIP5, the largest subunit of NoRC, at a single lysine residue, K633, adjacent to the TIP5 RNA-binding domain, and that SIRT1 (removes the acetyl group from K633. PMID: 19578370

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MYST (SAS/MOZ) family
  • 数据库链接：

    HGNC: 17933

    OMIM: 609912

    KEGG: hsa:84148

    STRING: 9606.ENSP00000406037

    UniGene: Hs.533803