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  4. ATP2A2 Recombinant Monoclonal Antibody

ATP2A2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA076873A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA076873A0HU diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.
    • Immunofluorescence staining of MCF7 with CSB-RA076873A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 509-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA076873A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • Uniprot No.：
    P16615
  • 基因名：
  • 别名：
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) (SR Ca(2+)-ATPase 2) (EC 3.6.3.8) (Calcium pump 2) (Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase), ATP2A2, ATP2B
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human ATP2A2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    20D1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes.; Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.
  • 基因功能参考文献：
    1. SGK3 a kinase transcriptionally regulated by estrogen receptor alpha (ERalpha) in breast cancer, sustains ERalpha signaling and drives the acquired aromatase inhibitors resistance by protecting against endoplasmic reticulum (EnR) stress-induced ERalpha downregulation and cell death through preserving SERCA2b function. PMID: 28174265
    2. Darier disease (DD) is a rare autosomal dominant skin disorder due to mutations in the ATP2A2 gene. PMID: 29142187
    3. SERCA2a gene transfer significantly improves left ventricle function and dimensions in doxorubicin-induced cardiomyopathy, suggesting LV-SERCA2a gene transfer an attractive treatment modality for doxorubicin-induced heart failure. PMID: 27203155
    4. Studies indicate that Darier disease (DD) is caused by mutations in the ATP2A2 gene, whereas the ATP2C1 gene is associated with Hailey-Hailey disease (HHD). PMID: 28035777
    5. Taken together, these results suggest that SERCA2 contributes to the migration of CCL21-activated Dendritic Cells as an important feature of the adaptive immune response and provide novel insights regarding the role of SERCA2 in Dendritic Cells functions. PMID: 27538371
    6. Novel mutations in Darier disease and association to self-reported disease severity PMID: 29028823
    7. VMP1 modulates SERCA2 activity to control endoplasmic reticulum contacts for autophagosome formation. PMID: 28890335
    8. The left atrium / right atrium expression ratio was significantly increased in Atrial fibrillation for SERCA2 - gene related to calcium uptake and release, and located on the sarcoplasmic reticulum membrane. PMID: 27494721
    9. Loss of SERCA2 impairs ER-to-Golgi transport of nascent DC. PMID: 28156030
    10. We propose that the increased SERCA1a expression indicates the existence and location of compensating mechanisms in ischemic muscle. PMID: 28648117
    11. Results show that ATP2A2 is variably expressed in astrocytoma tissues and its expression correlates with tumor grade. Its overexpression suppresses growth of astrocytoma cells. PMID: 28339043
    12. study identifies a novel splice acceptor site mutation in the ATP2A2 gene, in a family showing Darier disease PMID: 27595213
    13. Data suggest that mutations of the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) gene may lead to the occurrence of Darier disease (DD) in both familial and sporadic cases with DD. PMID: 27577212
    14. Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review) PMID: 26743715
    15. High SERCA2 expression is associated with colorectal cancer. PMID: 26608678
    16. Quantification of SERCA2 mRNA or protein expression levels revealed no differences in airway smooth muscle cells obtained from subjects with asthma compared to non-asthmatic controls. PMID: 25880173
    17. Inhibition of SERCA2 activity by curcumin disrupts the Ca(2+) homeostasis and thereby promotes apoptosis in ovarian cancer cells. PMID: 26607901
    18. The association between Darier disease and bipolar disorder is manifest also in the population, and data suggest that genetic variability within the ATP2A2 gene that causes Darier disease also confers susceptibility for bipolar disorder. PMID: 25213221
    19. ATP2A2 mutation was found in patients diagnosed with Darier disease. PMID: 26051059
    20. Two novel missense mutations, p.R603I and p.G749V, in the ATP2A2 gene in two families containing individuals diagnosed with Darier disease. PMID: 25872913
    21. study identified two heterozygous mutations in exon 12 of the ATP2A2 gene in two different Darier disease families PMID: 24552170
    22. Case Report: ATP2A2 missense mutation at one of the calcium-binding residues causing Darier's disease. PMID: 24979555
    23. We report here two Chinese Han patients with sporadic Darier disease; mutation analysis of ATP2A2 supported the genetic diagnosis. PMID: 25283811
    24. SPCA1 and sarco/endoplasmic reticulum Ca(2+) ATPase2 (SERCA2) encoded by ATP2A2 are two essential calcium pumps needed for Ca(2+) homeostasis maintenance in keratinocytes. PMID: 25256005
    25. SERCA and PMCA pump activities are strongly affected by the localization of F508del-CFTR protein. PMID: 25661196
    26. Evidence obtained from different diabetes models has suggested a role for advanced glycation end products formation, oxidative stress and increased O-GlcNAcylation in the lowered SERCA2 expression observed in diabetic cardiomyopathy PMID: 25270119
    27. Gene therapy in heart failure. SERCA2a as a therapeutic target. PMID: 25327883
    28. PDE3A is part of a SERCA2 signaling complex in cardiac myocytes. PMID: 25593322
    29. ERK activation is sufficient to reduce SERCA2 mRNA. PMID: 25008120
    30. A large Chinese family have a novel missense mutation in Darier disease. PMID: 23621824
    31. Exposure of normal keratinocytes to the SERCA2 inhibitor thapsigargin recapitulated these abnormalities, supporting the role of loss of SERCA2 function in impaired desmosome and adherens junction formation. PMID: 24390139
    32. a novel role of SERCA2b in facilitating the blockade of human liposarcoma differentiation PMID: 24508653
    33. palmitoylated calnexin interacts with sarcoendoplasmic reticulum Ca(2+) transport ATPase 2b and this interaction determines endoplasmic reticulum Ca(2+) content and the regulation of endoplasmic reticulum-mitochondria Ca(2+) crosstalk. PMID: 23843619
    34. I-1 and sarco/endoplasmic reticulum Ca2+ -ATPase synergistically induce the vascular smooth muscle cell contractile phenotype. PMID: 24249716
    35. the protective effect of hepatic stimulator substance against endoplasmic reticulum stress may be associated with the removal of reactive oxygen species to restore the activity of the sarco-endoplasmic reticulum Ca(2+)-ATPase. PMID: 24284796
    36. Novel ATP2A2 mutations in a large sample of individuals with Darier disease. PMID: 23356892
    37. The results demonstrate that (*)NO-mediated activation of SERCA2b via S-glutathiolation of cysteine-674 is required for VEGF-induced EC Ca(2+) influx and migration, and establish redox regulation of SERCA2b as a key component in angiogenic signaling. PMID: 22472004
    38. Downregulation of SERCA2a plays a critical role in modulating vascular and right ventricular phenotype associated with pulmonary arterial hypertension. PMID: 23804254
    39. The gene encodes a calcium-ATPase type 2 in the sarco-/endoplasmic reticulum (SERCA2), which belongs to the large family of P-type cation pumps. PMID: 23337962
    40. By introducing SERCA2 gene to an experimental heart failure model, cardiac functions and prognosis were improved and cardiac remodelling suppressed. SERCA2 is believed to be an important key to correct molecular network in heart failure. PMID: 23229631
    41. The protein SERCA2 expression was decreased and 43 miRNAs were deregulated in infarcted myocardium compared to corresponding remote myocardium. PMID: 23066896
    42. Shared histopathological features of acrokeratosis verruciformis of Hopf with Darier disease suggest that they are allelic disorders with variable expression of the same disease. Identical mutations in ATP2A2 in both diseases were not reported to date. PMID: 22814319
    43. we report one novel heterozygous splice site mutation of ATP2A2 gene and one previously described nonsense mutation. PMID: 22909361
    44. a higher Ca(2+) affinity of SERCA2b relative to other SERCA isoforms, not only on the cytosolic side, but also on the luminal side. PMID: 23024360
    45. SERCA2 dysregulation is a pathogenic event in 22q11 deletion syndrome (22q11DS) and schizophrenia. PMID: 23055483
    46. We report two novel mutations of the ATP2A2 gene in two Chinese families with Darier disease. PMID: 22329366
    47. Review article highlights the regulatory mechanisms of cardiac contractility by way of the multimeric SERCA/phospholamban (PLN)-ensemble. PMID: 22679139
    48. functional interactions of SERCA2b and Bcl-2 in the cell may be modulated by HSP70 and other chaperones and stress-regulated proteins. PMID: 22360692
    49. Loss of SERCA2 expression is observed in human diabetes and occurs within the context of PPAR-gamma phosphorylation and CDK5 activation. PMID: 22240811
    50. SERCA2-controlled Ca(2)+-dependent keratinocyte adhesion and differentiation is mediated via the sphingolipid pathway. PMID: 22277942

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  • 相关疾病：
    Acrokeratosis verruciformis (AKV); Darier disease (DD)
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily
  • 组织特异性：
    Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart a
  • 数据库链接：

    HGNC: 812

    OMIM: 101900

    KEGG: hsa:488

    STRING: 9606.ENSP00000440045

    UniGene: Hs.506759