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AHCY Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA176658A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate
      All lanes: AHCY antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48, 45 kDa
      Observed band size: 36-55 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P23526
  • 基因名：
    AHCY
  • 别名：
    Adenosylhomocysteinase (AdoHcyase) (EC 3.3.1.1) (S-adenosyl-L-homocysteine hydrolase), AHCY, SAHH
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human AHCY
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    13H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • 基因功能参考文献：
    1. Results uncover a H19/SAHH circuit involving gene-methylation alterations by carcinogen BaP. PMID: 29772428
    2. We investigated previously assumed interaction with AHCY-like-1 protein (AHCYL1), a paralog of AHCY. Indeed, significant interaction between both proteins exists. Additionally, silencing AHCYL1 leads to moderate inhibition of nuclear export of endogenous AHCY. PMID: 28647132
    3. We have validated the vectors and confirmed self-association of AHCY, AHCYL1, and galectin-3. In a high-throughput BiFC screen, we identified new AHCY interaction partners: galectin-3 and PUS7L. We also describe additional steps in protein interaction analysis, applied for AHCY-galectin-3 interaction PMID: 27455993
    4. In order to enable the development of small molecule AHCY inhibitors as targeted cancer therapeutics we developed an assay based on a RapidFire high-throughput mass spectrometry detection system, which allows the direct measurement of AHCY enzymatic activity. PMID: 28533090
    5. SAH hydrolase deficiency can remain asymptomatic in childhood, and the disorder can be associated with early onset hepatocellular carcinoma. PMID: 26527160
    6. H19 knockdown activates SAHH, leading to increased DNMT3B-mediated methylation of an lncRNA-encoding gene Nctc1 within the Igf2-H19-Nctc1 locus. PMID: 26687445
    7. S-adenosylhomocysteine hydrolase is regulated by lysine acetylation PMID: 25248746
    8. SAHH can promote apoptosis, inhibit migration and adhesion of ESCC cells suggesting that it may be involved in carcinogenesis of the esophagus. PMID: 24430301
    9. A fluorescence-based assay for the measurement of S-adenosylhomocysteine hydrolase activity in biological samples. PMID: 23079506
    10. The simulations of ligand-induced transition revealed that the signal of intrasubunit closure dynamics is transmitted to form intersubunit contacts, which in turn invoke a precise alignment of active site. PMID: 22023331
    11. Five active site residues (E156, N181, K186, D190, N191) of AdoHcy hydrolase have been individually mutated to alanine and each engineered enzyme characterized with respect to its redox partial reaction and elimination/addition partial reaction. PMID: 12069606
    12. a discussion of its hydrolytic activity (review) PMID: 12369977
    13. Maintenance of ionizable active-site residues in catalytically suitable protonation states in closed forms of placental AHCY may be assisted by a water chain, stabilized by Asp182, that can import and export protons from and to the environment. PMID: 12590576
    14. AdoHcy is involved in adenosine-induced apoptosis by altering gene expression. PMID: 17097637
    15. R38W and G123R amino-acid exchanges did not bring about major changes to the catalytic rates. However, circular dichroism analysis showed that both polymorphisms effect the thermal stability. PMID: 17164794
    16. SAHH, which is diffuse in the cytoplasm of nonmotile Dictyostelium amoebae and human neutrophils, concentrates with F-actin in pseudopods at the front of motile, chemotaxing cells PMID: 17172447
    17. In the case of Hs-SAHH, the slow-binding phase terminates in micromolar affinity, but over a period of hours, the dissociation rate constant decreases until the final equilibrium affinity is in the nanomolar range. PMID: 17447732
    18. We found clinically relevant levels of Hcy (0-500 microM) induced elevation of SAH, declination of SAM and SAM/SAH ratio and reduced expression of SAHH and MBD2, but increased activity of DNMT3a and DNMT3b affecting DNA methylation PMID: 17688412
    19. The mechanism of action of copper on this enzyme sugges a regulative role for copper on the intracellular activity. PMID: 17892301
    20. consider changes in charge and the sterical incompatibility in mutant p.A89V protein as main reason for enzyme malfunction with AdoHcyase deficiency as consequence PMID: 18211827
    21. SAHH from Homo sapiens (Hs-SAHH) and from the parasite Trypanosoma cruzi (Tc-SAHH) are very similar in structure and catalytic properties but differ in the kinetics and thermodynamics of association and dissociation of the cofactor NAD (+) PMID: 18393535
    22. Streptococcal pyrogenic exotoxin B (SPE B) potentially causes immunosuppression by cleaving human S-adenosylhomocysteine hydrolase (AdoHcyase). PMID: 18522500
    23. increased plasma concentration in patients with idiopathic thrombocytopenic purpura PMID: 18683034
    24. SAHH mRNA was lost in 50% of tumor tissues from 206 patients with different kinds of tumors in comparison with normal tissue counterparts. Moreover, SAHH protein was also affected in some colon cancers PMID: 18713839
    25. These data show that 2-5-fold enhanced AdoHcyase activity is well tolerated by the cell, while greatly enhanced AdoHcyase activity results in adenosine-induced apoptosis. PMID: 18769049
    26. S-adenosylhomocysteine hydrolase PMID: 19619139

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  • 相关疾病：
    Hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD)
  • 亚细胞定位：
    Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • 蛋白家族：
    Adenosylhomocysteinase family
  • 数据库链接：

    HGNC: 343

    OMIM: 180960

    KEGG: hsa:191

    STRING: 9606.ENSP00000217426

    UniGene: Hs.388004