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vpx Antibody

  • 货号：
    CSB-PA325553LA01HLU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Human immunodeficiency virus type 2 subtype A vpx Polyclonal antibody
  • Uniprot No.：
    P18099
  • 基因名：
    vpx
  • 别名：
    vpxProtein Vpx antibody; Viral protein X antibody; X ORF protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human immunodeficiency virus type 2 subtype A
  • 免疫原：
    Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx protein (1-113AA)
  • 免疫原种属：
    Human immunodeficiency virus type 2 subtype A
  • 标记方式：
    Non-conjugated

    本页面中的产品，vpx Antibody (CSB-PA325553LA01HLU)，的标记方式是Non-conjugated。对于vpx Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA325553LB01HLU vpx Antibody, HRP conjugated ELISA
    FITC CSB-PA325553LC01HLU vpx Antibody, FITC conjugated
    Biotin CSB-PA325553LD01HLU vpx Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.
  • 基因功能参考文献：
    1. These results indicate that Vpx, in addition to SAMHD1, overcomes a previously unappreciated restriction for lentiviruses at the level of reverse transcription (RT)that acts independently of dNTP concentrations and is specific to resting CD4 T cells. PMID: 28228523
    2. zinc binding appears to mitigate flexibility of the three-helix fold of Vpx, thereby preventing dysfunction. PMID: 28284276
    3. Data indicate that proline residue P109 within the C-terminal poly-proline motif (PPM) of Vpx protein plays a unique role in the regulation of SAM domain and HD domain 1 protein SAMHD1 degradation. PMID: 25936766
    4. HIV-2 Vpr can trigger G2 cell cycle arrest through either CUL4A or CUL4B. PMID: 24719410
    5. Vpx expression results in decreased promoter binding activity of IRF5 PMID: 24532789
    6. Poly-proline motif in Vpx is critical for its efficient expression in cells. PMID: 24114794
    7. Data suggest that, in an evolutionary model of virus-host interactions, binding of Vpx/Vpr with SAMHD1 (and subsequent degradation of SAMHD1 [SAM domain/HD domain-containing protein 1]) exhibits dynamic requirements that have toggled back and forth. PMID: 23874202
    8. the crystal structure of a ternary complex of Vpx with the human E3 ligase substrate adaptor DCAF1 and the carboxy-terminal region of human SAMHD1 PMID: 24336198
    9. direct down-modulation of Vpx catalytic activity, mediated by the same binding event that leads to SAMHD1 recruitment to the E3 ubiquitin ligase for proteasome-dependent degradation PMID: 23677995
    10. Effective immune control of viral replication in HIV-2-infected individuals is not associated with increased Vpx-mediated degradation of SAMHD1. PMID: 23497283
    11. there are several Vpx residues required for SAMHD1 degradation PMID: 23076149
    12. The Vpx lentiviral accessory protein targets human SAMHD1 for degradation in the nucleus. PMID: 22973040
    13. Vpx targets SAMHD1 for degradation in a viral strategy to control cellular deoxynucleotide levels for efficient replication PMID: 22069334
    14. structure and cytopathogenic activity PMID: 16153874
    15. These studies map critical residues of the Vpx nuclear localization signal that are required for efficient infection of non-dividing cells. PMID: 16325220
    16. We found that insertion within the polyproline-containing C-terminus destabilizes nuclear localization, whereas mutating a second helix in the central domain disrupts viral packaging. PMID: 16457868
    17. results indicated that Vpx and Vpr of HIV-2 may cooperatively contribute to virion infectivity without affecting virion morphogenesis PMID: 16953064
    18. The results here clearly demonstrated that the entire Vpx protein is critical for reverse transcription of the HIV-2 genome in human monocyte-derived macrophages. PMID: 18495778
    19. when transferred in the context of a replication-competent viral clone, Vpx was required for replication in dendritic cells PMID: 18829761
    20. Vpx diverts the Cul4A-DDB1(DCAF1) ligase to inactivate an evolutionarily conserved factor, which restricts macrophage infection by HIV-2. PMID: 19264781

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  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Lentivirus VPX protein family
  • 数据库链接：

    KEGG: vg:1724714