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pum Antibody

  • 中文名称：
    pum兔多克隆抗体
  • 货号：
    CSB-PA326575XA01DLU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: pum Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 30 kDa
      Observed band size: 30 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P25822
  • 基因名：
    pum
  • 别名：
    Maternal protein pumilio pum CG9755
  • 反应种属：
    Drosophila melanogaster (Fruit fly)
  • 免疫原：
    Recombinant Drosophila melanogaster (Fruit fly) pum protein (1091-1330aa)
  • 免疫原种属：
    Drosophila melanogaster (Fruit fly)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-chromatography
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Mediates post-transcriptional silencing of E2f mRNA by binding to its 3'-UTR and promoting miRNA regulation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.
  • 基因功能参考文献：
    1. The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described. PMID: 27729296
    2. Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity. PMID: 27482653
    3. reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation. PMID: 26438601
    4. Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system. PMID: 25896902
    5. Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches PMID: 22391052
    6. Pumilio complexes repress dE2F1 levels PMID: 22345517
    7. Data show that the extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon. PMID: 21896737
    8. The studies suggest that, in cystoblasts, Bam and Bgcn may directly inhibit Pum/Nos activity to promote differentiation of germ line stem cells. PMID: 20018853
    9. affects anterior determination in drosophila embryo; temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR PMID: 12015297
    10. a translaitonal repressor that regulates neuronal excitability PMID: 12136020
    11. Pumilio represses translation of differentiation-promoting mRNAs in germline stem cells. PMID: 15668176
    12. A characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO was identified. PMID: 16537387
    13. Regulatory interplay between Nanos and Pum could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses. PMID: 19403823

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule.
  • 数据库链接：

    KEGG: dme:Dmel_CG9755

    STRING: 7227.FBpp0305823

    UniGene: Dm.1115