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nusG Antibody

  • 货号：
    CSB-PA365293XA01ENV
  • 规格：
    ￥880
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:nusGAntibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 23 kDa
      Observed band size: 23 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli (strain K12) nusG Antibody Polyclonal antibody
  • Uniprot No.：
    P0AFG0
  • 基因名：
    nusG Antibody
  • 别名：
    Transcription termination/antitermination protein NusG nusG b3982 JW3945
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli (strain K12)
  • 免疫原：
    Recombinant Escherichia coli (strain K12) nusG protein (2-181aa)
  • 免疫原种属：
    Escherichia coli (strain K12)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNAP, probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.
  • 基因功能参考文献：
    1. The comprehensive mapping of the upstream fork junction by base analogue fluorescence and nucleic acids crosslinking suggests that NusG inhibits backtracking by stabilizing the minimal transcription bubble. PMID: 27697152
    2. NusG acts as both a positive and a negative regulator of Rho in the course of the bacterial transcription termination. (Review) PMID: 27023849
    3. NusG is required for efficient termination at minority subsets (~20%) of both antisense and sense Rho-dependent terminators with lower C/G ratio sequences PMID: 23207917
    4. E. coli NusG inhibits backtracking and accelerates pause-free transcription by promoting forward translocation of RNA polymerase. PMID: 20381500
    5. findings suggest that the mini-domain serves as a structural element for an interaction rather than as a site for residue-specific contacts PMID: 15890417

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  • 蛋白家族：
    NusG family
  • 数据库链接：

    KEGG: ecj:JW3945

    STRING: 316385.ECDH10B_4170