Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. ligA Antibody

ligA Antibody

  • 货号：
    CSB-PA322745HA01ENV
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: DNA ligase antibody at 2μg/ml + DH5a whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli ligA Polyclonal antibody
  • Uniprot No.：
    P15042
  • 基因名：
    ligA
  • 别名：
    ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli DNA ligase protein (1-671AA)
  • 免疫原种属：
    Escherichia coli
  • 标记方式：
    Non-conjugated

    本页面中的产品，ligA Antibody (CSB-PA322745HA01ENV)，的标记方式是Non-conjugated。对于ligA Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA322745HB01ENV ligA Antibody, HRP conjugated ELISA
    FITC CSB-PA322745HC01ENV ligA Antibody, FITC conjugated
    Biotin CSB-PA322745HD01ENV ligA Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
  • 基因功能参考文献：
    1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
    2. Here the s show that replication without ligase kills after a short resistance period. PMID: 22582878
    3. temperature sensitive mutant characterized PMID: 15583169
    4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
    5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
    6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
    7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356

    显示更多

    收起更多

  • 蛋白家族：
    NAD-dependent DNA ligase family, LigA subfamily
  • 数据库链接：

    KEGG: ecj:JW2403

    STRING: 316385.ECDH10B_2576