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acrA Antibody

  • 货号：
    CSB-PA360036XA01ENV
  • 规格：
    ￥880
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:acrA Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31.4 kDa
      Observed band size: 32 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli (strain K12) acrA Antibody Polyclonal antibody
  • Uniprot No.：
    P0AE06
  • 基因名：
    acrA Antibody
  • 别名：
    Multidrug efflux pump subunit AcrA (AcrAB-TolC multidrug efflux pump subunit AcrA) (Acridine resistance protein A) acrA lir mtcA b0463 JW0452
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli (strain K12)
  • 免疫原：
    Recombinant Escherichia coli (strain K12) acrA protein (126-397aa)
  • 免疫原种属：
    Escherichia coli (strain K12)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex with broad substrate specificity that uses the proton motive force to export substrates. This subunit may act as an adapter protein that links AcrB and TolC stably together. It is elongated in shape, being long enough to span the periplasm.
  • 基因功能参考文献：
    1. The results underscore the importance of the periplasmic turn 1 of TolC in the functional assembly of the tripartite efflux complex and AcrA in transitioning TolC from its closed to open state. PMID: 20132445
    2. Our results indicate functional interactions between the AcrA -hairpin tip region and the TolC aperture tip region in the assembly of the functional tripartite efflux pump AcrAB-TolC. PMID: 20581201
    3. Data show that the AcrA-mediated suppression of mutant TolC phenotypes and stabilization of mutant TolC were dependent on AcrB, reflecting the existence of a functional complex between TolC and AcrAB in vivo. PMID: 15491355
    4. TolC-AcrA interaction demonstrated via cross-linking studies PMID: 15576805
    5. The observed pulsating behavior in inducible transcription of acrA is a systems property arising from the dependence of gene expression on multidrug resistance determinants. PMID: 16461398
    6. AcrS represses the gene expression of multidrug efflux genes, acrA and acrB PMID: 18567659
    7. Data suggest that recruitment of TolC by various MFPs AcrA, MacA, and EmrA is determined not only by kinetics of MFP-TolC interactions but also by oligomerization kinetics of MFPs and pH. PMID: 19805313

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  • 亚细胞定位：
    Cell inner membrane; Lipid-anchor. Note=An unlipidated version of this protein (directed to the periplasm by the OmpA signal sequence) functions normally.
  • 蛋白家族：
    Membrane fusion protein (MFP) (TC 8.A.1) family
  • 数据库链接：

    KEGG: ecj:JW0452

    STRING: 316385.ECDH10B_0419