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YWHAG Antibody

  • 货号：
    CSB-PA948368
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 γ antibody.
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 γ antibody.
    • Immunofluorescence analysis of COS7 cells, using 14-3-3 γ antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) YWHAG Polyclonal antibody
  • Uniprot No.：
    P61981
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from internal of Human 14-3-3 γ.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 基因功能参考文献：
    1. 14-3-3gamma protein directly interacts with the kinase domain of CaMKK2 and the region containing the inhibitory phosphorylation site Thr(145) within the N-terminal extension. CaMKK isoforms differ in their 14-3-3-mediated regulations and the interaction between 14-3-3 protein and the N-terminal 14-3-3-binding motif of CaMKK2 might be stabilized by small-molecule compounds. PMID: 29649512
    2. Findings indicate the roles and molecular mechanisms of 14-3-3GAMMA protein (14-3-3gamma) in epithelial-mesenchymal transition (EMT), migration, and neoplasm invasion. PMID: 29848704
    3. Study demonstrated a mechanism by which 14-3-3gamma restricts centrosome duplication to once per cell cycle, by inhibiting cdc25C and cdk1 activation resulting in decrease phosphorylation of T199 in NPM1. Also, loss of 14-3-3gamma causes centrosome over-duplication, centrosome clustering and tumor formation in mice. PMID: 27253419
    4. Study identified 14-3-3gamma as a binding partner for segment a of ANO1 and enhanced the surface expression of ANO1. In addition, its gene silencing of 14-3-3gamma inhibited migration and invasion of these glioblastoma cell lines. PMID: 27212225
    5. These results suggest that a major role of 14-3-3gamma in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation. PMID: 29253567
    6. hypermethylation of the 14-3-3 gene promoter accounts for the decreased 14-3-3 gamma in uterine leiomyomas and that such a low level of expression may be involved in the pathogenesis of leiomyomas PMID: 27100507
    7. MiR-217 directly targeted 3'UTR of YWHAG and suppressed the expression of YWHAG. PMID: 28126486
    8. Data indicate angiopoietin-like 4 (ANGPTL4) as a key player that coordinates an increase in cellular energy flux crucial for EMT via an ANGPTL4/14-3-3gamma signaling axis. PMID: 28745316
    9. 14-3-3gamma regulates the differentiation ability of CPNE1 through the binding with C2A domain of CPNE1 in HiB5 cells. PMID: 28412242
    10. YWHAG de novo mutations cause early onset epilepsy, including epileptic encephalopathies and intellectual disability. PMID: 28777935
    11. Study found that the overexpression of 14-3-3gamma in utero in the developing mouse cortex results in delays in pyramidal neuron migration. PMID: 27288018
    12. The present study revealed the tumor suppressive role of miR-509-5p in non-small cell lung cancer (NSCLC) by targeting YWHAG, suggesting that miR-509-5p/YWHAG axis might be considered as a novel and potential target for clinical diagnosis and therapeutics of NSCLC. PMID: 27894843
    13. 14-3-3 protein expression was quantitatively analyzed in cerebrospinal fluid of 231 sporadic Creutzfeldt-Jakob disease and 2035 control patients. PMID: 25947081
    14. Data suggest that miR-181b-3p functions as a metastasis activator by promoting Snail-induced epithelial-mesenchymal transition in breast cancer cells by directly targeting YWHAG. PMID: 27102539
    15. Loss of p53 function may result in upregulation of 14-3-3gamma in lung cancers PMID: 25384678
    16. Changes for CRMP2, TCP1epsilon, TPM2 and 14-3-3gamma were confirmed in experimental tumors and in a series of 28 human SI-NETs. PMID: 25224486
    17. These results showed that the cell surface expression of TRPM4 channels is mediated by 14-3-3gamma binding. PMID: 25047048
    18. Proteomics analysis show that Ser40 of TH protein does not significantly contribute to the binding of 14-3-3gamma, and rather has reduced accessibility in the TH:14-3-3gamma complex. PMID: 24947669
    19. physiological changes in phosphate anions concentration can modulate affinity and specificity of interaction of 14-3-3 with its multiple targets and therefore the actual phosphointeractome of 14-3-3. PMID: 23977325
    20. High 14-3-3gamma expression was seen in 59.5% of non-small cell lung cancers. PMID: 24870749
    21. miR-141/YWHAG and miR-520e/RAB11A are two potential miRNA/protein target pairs associated with severe obesity. PMID: 22537031
    22. The normalisation capability of 14-3-3 gamma was superior to traditional LC in quantifying Western blot signals of the platelet AD-biomarker Monoamine Oxidase B of patient versus controls. PMID: 24284060
    23. 14-3-3gamma was induced in B cells by T-dependent and T-independent primary CSR-inducing stimuli in vitro. PMID: 23851690
    24. The region surrounding pSer19 of Tyrosine hydroxylase adopts an extended conformation in the 14-3-3gamma-bound state, whereas adopts a bent conformation when free in solution. PMID: 24055376
    25. Mitotic Plk1 activity is regulated not only by Plk1-Thr210 phosphorylation, but also by Plk1 binding to 14-3-3gamma following Plk1-Ser99 phosphorylation downstream of the PI3K-Akt signalling pathway. PMID: 23695676
    26. high 14-3-3gamma expression is associated with poor survival in NPC patients. Thus, this study has identified that the 14-3-3gamma involves in the carcinogenesis of NPC. PMID: 23500129
    27. Increased expression of 14-3-3gamma in breast cancer is associated significantly with tumor progression and poor prognosis. PMID: 22658894
    28. The peripheral binding of 14-3-3gamma to membranes involves isoform-specific histidine residues. PMID: 23189152
    29. Using individual amino acid substitutions within the 14-3-3gamma VRII, we identified two residues required for and two contributing to the gamma-specific phenotypes. PMID: 23115241
    30. 14-3-3gamma protein binds strongly to long DNA targets and shows very strong preference for supercoiled DNA. PMID: 22856523
    31. hypoxia can activate p53 through inactivation of MDMX by the ATR-Chk1-MDMX-14-3-3gamma pathway. PMID: 22556425
    32. description of a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3gamma PMID: 22366688
    33. Increased expression of 14-3-3 gamma in lung cancer coincides with loss of functional p53. PMID: 21867493
    34. results support that membrane binding involves the non-conserved, convex area of 14-3-3gamma, and that Trp residues do not intercalate in the bilayer. PMID: 21420405
    35. a new role for 14-3-3gamma in protecting p21 from MDMX-mediated proteasomal turnover, which may partially account for DNA damage-induced elevation of p21 levels independent of p53. PMID: 21148311
    36. data do not exclude the possibility that YWHAG loss of function is also sufficient to cause neurological phenotypes PMID: 21109226
    37. Elevated expression of 14-3-3gamma in human hepatocellular carcinoma predicts extrahepatic metastasis and worse survival PMID: 20870266
    38. 14-3-3 protein gamma forms a complex with Checkpoint kinase 1 phosphorylated at Ser296, but not at ATR sites (Ser317 and Ser345). PMID: 20639859
    39. Abundant level of the phosphorylated FOXO1, its impaired nucleocytoplasmic shuttling, and the lowered expression of 14-3-3 protein in leiomyoma induces a shift in the cellular machinery toward a prosurvival execution program. PMID: 19772960
    40. 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma. PMID: 20388496
    41. Zebrafish gene knockdowns imply roles for human YWHAG in infantile spasms and cardiomegaly. PMID: 20146355
    42. Aberrant expression of signaling-related proteins 14-3-3 gamma and RACK1 in fetal Down syndrome brain (trisomy 21). PMID: 11824616
    43. 14-3-3 suppresses importin alpha/beta-dependent nuclear localization of Thr157-phosphorylated p27, suggesting implications for cell cycle disorder in Akt-activated cancer cells. PMID: 15057270
    44. multiple interactions of AICD with FE65 and 14-3-3gamma modulate FE65-dependent gene transactivation PMID: 16223726
    45. Interaction of human 14-3-3gamma with the small heat shock protein Hsp20 was analyzed by means of size-exclusion chromatography and chemical crosslinking. PMID: 17109079
    46. Both single marker and haplotype analyses were negative for the Ywhag gene association with smoking initiation and nicotine dependence. PMID: 17284169
    47. our data indicate that 14-3-3gamma may contribute to tumorigenesis by promoting genomic instability. PMID: 17394238
    48. Detection of only 2 (14-3-3 eta and gamma) out of 7 different isoforms in synovial fluid suggests they are specific to the site of joint inflammation PMID: 17611984
    49. Report reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics. PMID: 18054924
    50. human 14-3-3gamma binds to the ERK1/2 molecular scaffold KSR1, which is mediated by the C-terminal stretch of 14-3-3gamma PMID: 18426801

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    14-3-3 family
  • 组织特异性：
    Highly expressed in brain, skeletal muscle, and heart.
  • 数据库链接：

    HGNC: 12852

    OMIM: 605356

    KEGG: hsa:7532

    STRING: 9606.ENSP00000306330

    UniGene: Hs.744840