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YARS Antibody

  • 货号：
    CSB-PA00465A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western blot
      All lanes: YARS antibody at 2µg/ml + 293T whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/15000 dilution
      Predicted band size: 60 kDa
      Observed band size: 60 kDa
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00465A0Rb at dilution of 1:100
    • Immunofluorescence staining of SH-SY5Y cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) YARS Polyclonal antibody
  • Uniprot No.：
    P54577
  • 基因名：
    YARS
  • 别名：
    CMTDIC antibody; SYYC_HUMAN antibody; Tyrosine tRNA ligase, cytoplasmic antibody; Tyrosine tRNA ligase 1, cytoplasmic antibody; Tyrosyl tRNA synthetase antibody; Tyrosyl--tRNA ligase antibody; Tyrosyl-tRNA synthetase, cytoplasmic antibody; TyrRS antibody; yars antibody; YRS antibody; YTS antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Tyrosine--tRNA ligase, cytoplasmic protein (2-528AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，YARS Antibody (CSB-PA00465A0Rb)，的标记方式是Non-conjugated。对于YARS Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA00465B0Rb YARS Antibody, HRP conjugated ELISA
    FITC CSB-PA00465C0Rb YARS Antibody, FITC conjugated
    Biotin CSB-PA00465D0Rb YARS Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • 基因功能参考文献：
    1. conclusion is further supported by a positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production. PMID: 29289698
    2. Platelet replenishment by YRS(ACT) is independent of thrombopoietin (TPO), as evidenced by expansion of the megakaryocytes from induced pluripotent stem cell-derived hematopoietic stem cells from a patient deficient in TPO signaling. PMID: 30104364
    3. These YARS variants occur in the catalytic domain and the C-terminal domain, respectively. Mutations in YARS have been previously associated with an autosomal dominant form of Charcot-Marie-Tooth (CMT); our findings suggest the disease spectrum associated with YARS dysregulation is broader than peripheral neuropathy. PMID: 27633801
    4. Studied the structural effect of three Charcot-Marie-Tooth disease-causing mutations in tyrosyl-tRNA synthetase. The mutations do not induce changes in protein secondary structures, or shared effects on oligomerization state and stability. However, all mutations provide access to a surface masked in the wild-type enzyme, and that access correlates with protein misinteraction. PMID: 28531329
    5. Data show that the internal deletion of tyrosyl-tRNA synthetase TyrRSDeltaE2-4 splice variants (SVs) gave an alternative, neomorphic dimer interface 'orthogonal' to that of native TyrRS. PMID: 26773056
    6. Expression of CMT-mutant tyrosyl-tRNA synthetase in Drosophila impairs protein translation. PMID: 26138142
    7. Computational modeling of molecular dynamics of G41R mutant form of human tyrosyl-tRNA synthetase, assosiated with Charcot-Marie-Tooth neuropathy has been presented. PMID: 27025069
    8. the association of rare YARS variant with late-onset autosomal dominant Charcot-Marie-Tooth neuropathy PMID: 24354524
    9. This study presents genetic evidence for common mutant-specific interactions between two CMT-associated aminoacyl-tRNA synthetases, lending support for a shared mechanism responsible for the synthetase-induced peripheral neuropathies. PMID: 24807208
    10. rhTyrRS promotes migration and aggregation of megakaryocytes to the bone marrow niche PMID: 24907514
    11. nuclear-localized TyrRS activates transcription factor E2F1 to upregulate the expression of DNA damage repair genes such as BRCA1 and RAD51. PMID: 25284223
    12. A major difference between the first- and second-generation tRNA synthetases (RSs) is that the second-generation RSs have an active site more compatible with tyrosine binding. PMID: 24611875
    13. The full length tyrosyl-tRNA synthetase lacks its cytokine activity because of the interactions between N-terminal and the C-terminal modules, which protect the ELR cytokine motif. PMID: 23334919
    14. Nuclear import of TyrRS is regulated by tRNA(Tyr). PMID: 22291016
    15. Dominant Intermediate Charcot-Marie-Tooth disorder is not due to a catalytic defect in tyrosyl-tRNA synthetase. PMID: 21732632
    16. Expression of tyrosyl-tRNA synthetase (YARS) DI-CMTC associated mutations (G41R, E196K,153-156delVKQV)in Drosophila leads to neuronal dysfunction. PMID: 19561293
    17. role in catalyzing tyrosyl-adenylate formation PMID: 11856731
    18. replacement of second lysine in KMSKS signature sequence by potassium PMID: 11927599
    19. role in inducing angiogenesis PMID: 11956181
    20. the KMSSS sequence in human tyrosyl-tRNA synthetase stabilizes the transition state for the tyrosine activation reaction by interacting with the pyrophosphate moiety of ATP PMID: 12016229
    21. The recently discovered proangiogenic role of a tyrosyl-tRNA synthetase fragment that stimulates immune cells and links translation to a major cell-signaling pathway is discussed in this review. PMID: 12416978
    22. The structure of human mini-TyrRS containing both the catalytic & the anticodon recognition domains, is reported to a resolution of 1.18 A. The spatial disposition of the anticodon recognition domain relative to the catalytic domain is unique. PMID: 12427973
    23. identification of two heterozygous missense mutations (G41R and E196K) and one de novo deletion (153-156delVKQV) in tyrosyl-tRNA synthetase (YARS) in three unrelated families affected with dominant intermediate Charcot-Marie-Tooth neuropathy PMID: 16429158
    24. Mutating a conserved tyrosine (Y341) that tethers a critical ELR motif in TyrRS resulted in subtle opening of the structure, and activation of cytokine functions, proving the possibility of constitutive gain-of-function mutations in tRNA ligases. PMID: 18096501
    25. Human tyrosyl-tRNA synthetase, where a catalytic-domain surface helix, next to the active site, was recruited for interleukin-8-like cytokine signaling. PMID: 19477417

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  • 相关疾病：
    Charcot-Marie-Tooth disease, dominant, intermediate type, C (CMTDIC)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I aminoacyl-tRNA synthetase family
  • 数据库链接：

    HGNC: 12840

    OMIM: 603623

    KEGG: hsa:8565

    STRING: 9606.ENSP00000362576

    UniGene: Hs.213264