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XRN2 Antibody

  • 中文名称：
    XRN2兔多克隆抗体
  • 货号：
    CSB-PA267042
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of COS7 cells using XRN2 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q9H0D6
  • 基因名：
    XRN2
  • 别名：
    XRN2 antibody; 5'-3' exoribonuclease 2 antibody; EC 3.1.13.- antibody; DHM1-like protein antibody; DHP protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Monkey
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human XRN2.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites.
  • 基因功能参考文献：
    1. Xrn2 depletion induces a transcriptional termination defect at the majority of protein-coding genes. PMID: 30035655
    2. depletion of NKRF, XRN2 or DHX15 impairs an early pre-rRNA cleavage step. PMID: 28115624
    3. XRN2 is a novel regulator of EMT that contributes to the metastatic processes in lung cancer through a novel miRNA regulatory mechanism. PMID: 28319071
    4. XRN2 has a role in R-loop resolution, suppression of replication stress, and maintenance of genomic stability PMID: 27437695
    5. Chromatin immunoprecipitation experiments further show that IL-1 stimulation leads to decrease in NKRF aa 421-429 phosphorylation and dissociation of NELF-E and XRN2 by concomitant resumption of transcription elongation of a synthetic reporter PMID: 26340924
    6. P-TEFb regulates termination by promoting chromatin recruitment and activation of a cotranscriptional RNA processing enzyme, Xrn2. PMID: 26728557
    7. The results suggest that CARF regulates early steps of pre-rRNA processing during ribosome biogenesis by controlling spatial distribution of XRN2 between the nucleoplasm and nucleolus. PMID: 26531822
    8. Kinetic competition between elongating pol II and the Xrn2 exonuclease is integral to termination of transcription on most human genes. PMID: 26474067
    9. found that the 3' fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5' ends by nuclear XRN2 after RNase H1-mediated cleavage PMID: 26159921
    10. The restriction of JFH1 and H77D hepatitis C virus replication by Xrn2 is likely indirect in nature and possibly linked to cytopathic effects of these robustly replicating viruses. PMID: 25673723
    11. Xrn2 has a cytoplasmic, antiviral function against HCV that is counteracted by HCV's subversion of miR-122 to form a protective oligomeric complex at the 5' end of the viral genome. PMID: 25121753
    12. Mammalian target of rapamycin protein regulates the diffusion of Xrn2 from the nucleolus to the nucleoplasm under heat stress conditions. PMID: 25128458
    13. hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582
    14. Microprocessor orchestrates the recruitment of termination factors Setx and Xrn2, and the 3'-5' exoribonuclease, Rrp6, to initiate RNAPII pausing and premature termination at the HIV-1 promoter through cleavage of the stem-loop RNA, TAR. PMID: 22980978
    15. Xrn2 associated with and co-transcriptionally degraded nascent beta-globin transcripts. Evidence was provided that many endogenous pre-mRNAs are also co-transcriptionally degraded by Xrn2 when their processing is inhibited by Spliceostatin A. PMID: 22522706
    16. Xrn2 has a role in control of transcription by RNA polymerase II. PMID: 22483619
    17. The results predict that senataxin is required for the resolution of R-loops formed downstream of the poly(A) signal, allowing Xrn2 recruitment and efficient Pol II transcriptional termination. PMID: 21700224
    18. Genetic variants regulating Xrn2 expression in cis are determinants of spontaneous lung tumor susceptibility in mice and have genetic equivalents in lung cancer susceptibility in human beings. PMID: 19915612
    19. co-transcriptional cleavage acts as a precursor to termination by presenting a free RNA 5' end that is recognized by the human 5' --> 3' exonuclease Xrn2 PMID: 15565158
    20. XRN2b is mainly expressed in blood leukocyte tissue, while XRN2a is detected in several human tissues and in human tumor tissues PMID: 16147866
    21. p54 is present along the length of genes, and small interfering RNA (siRNA)-mediated knockdown leads to defects in XRN2 recruitment and termination. PMID: 17639083
    22. Degradation of the downstream cleavage product (DCP) does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP both in vitro and in vivo. PMID: 18955505

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    5'-3' exonuclease family, XRN2/RAT1 subfamily
  • 组织特异性：
    Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 12836

    OMIM: 608851

    KEGG: hsa:22803

    STRING: 9606.ENSP00000366396

    UniGene: Hs.255932