WWC3 Antibody
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货号:CSB-PA890929NA01HU
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规格:¥440
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促销:
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图片:
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IHC image of CSB-PA890929NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of U251 cells with CSB-PA890929NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
All lanes: WWC3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 66 kDa
Observed band size: 123 kDa
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其他:
产品详情
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产品名称:Rabbit anti-Homo sapiens (Human) WWC3 Polyclonal antibody
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Uniprot No.:Q9ULE0
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基因名:WWC3
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别名:WWC3 antibody; KIAA1280 antibody; Protein WWC3 antibody
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宿主:Rabbit
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反应种属:Human
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免疫原:Peptide sequence from Human Protein WWC3 protein (65-85AA)
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
本页面中的产品,WWC3 Antibody (CSB-PA890929NA01HU),的标记方式是Non-conjugated。对于WWC3 Antibody,我们还提供其他标记。见下表:
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克隆类型:Polyclonal
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抗体亚型:IgG
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纯化方式:Antigen Affinity Purified
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, WB, IHC, IF
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推荐稀释比:
Application Recommended Dilution WB 1:500-1:2000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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