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VPS25 Antibody

  • 货号：
    CSB-PA887108LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: VPS25 antibody at 3µg/ml
      Lane 1: Mouse kidney tissue
      Lane 2: HL60 whole cell lysate
      Lane 3: THP-1 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 21 kDa
      Observed band size: 21 kDa
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA887108LA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA887108LA01HU at dilution of 1:100
    • Immunofluorescence staining of Hela cells with CSB-PA887108LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) VPS25 Polyclonal antibody
  • Uniprot No.：
    Q9BRG1
  • 基因名：
  • 别名：
    Dermal papilla derived protein 9 antibody; Dermal papilla-derived protein 9 antibody; DERP9 antibody; EAP20 antibody; ELL associated protein of 20 kDa antibody; ELL-associated protein of 20 kDa antibody; ESCRT-II complex subunit VPS25 antibody; FAP20 antibody; HGNC:28122 antibody; hVps25 antibody; MGC10540 antibody; Vacuolar protein sorting 25 homolog (S. cerevisiae) antibody; Vacuolar protein sorting associated protein 25 antibody; Vacuolar protein-sorting-associated protein 25 antibody; VPS25 antibody; VPS25_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Vacuolar protein-sorting-associated protein 25 protein (1-176AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，VPS25 Antibody (CSB-PA887108LA01HU)，的标记方式是Non-conjugated。对于VPS25 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA887108LB01HU VPS25 Antibody, HRP conjugated ELISA
    FITC CSB-PA887108LC01HU VPS25 Antibody, FITC conjugated
    Biotin CSB-PA887108LD01HU VPS25 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
  • 基因功能参考文献：
    1. mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body PMID: 16371348
  • 亚细胞定位：
    Cytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
  • 蛋白家族：
    VPS25 family
  • 组织特异性：
    Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.
  • 数据库链接：

    HGNC: 28122

    OMIM: 610907

    KEGG: hsa:84313

    STRING: 9606.ENSP00000253794

    UniGene: Hs.500165