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USP5 Antibody

  • 货号：
    CSB-PA025742LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: USP5 antibody at 10µg/ml
      Lane 1: Mouse brain tissue
      Lane 2: A549 whole cell lysate
      Lane 3: MCF-7 whole cell lysate
      Lane 4: A375 whole cell lysate
      Lane 5: U251 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 96, 94 kDa
      Observed band size: 96 kDa
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA025742LA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA025742LA01HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) USP5 Polyclonal antibody
  • Uniprot No.：
    P45974
  • 基因名：
    USP5
  • 别名：
    Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody; Ubiquitin thioesterase 5 antibody; Ubiquitin thiolesterase 5 antibody; Ubiquitin-specific-processing protease 5 antibody; UBP5_HUMAN antibody; Usp5 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Ubiquitin carboxyl-terminal hydrolase 5 protein (201-500AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，USP5 Antibody (CSB-PA025742LA01HU)，的标记方式是Non-conjugated。对于USP5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA025742LB01HU USP5 Antibody, HRP conjugated ELISA
    FITC CSB-PA025742LC01HU USP5 Antibody, FITC conjugated
    Biotin CSB-PA025742LD01HU USP5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
  • 基因功能参考文献：
    1. USP5 regulates c-Maf stability and multiple myeloma cell survival. PMID: 28933784
    2. study demonstrates that USP5 plays a critical role in tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein, and provides a rationale for USP5 being a potential therapeutic approach against pancreatic ductal adenocarcinoma PMID: 28807830
    3. Taken together, our study for the first time clarified that the E3 ligase Smurf1 regulates USP5 protein stability and USP5-mediated TNF-alpha production through the ubiquitin proteasome pathway. PMID: 27133717
    4. Used IM-MS analysis to study conformational flexibility of the multidomain deubiquitinating enzyme ubiquitin specific protease 5 (USP5). PMID: 25641936
    5. Charge-state distribution and ion mobility analysis revealed that both mono- and tetra-ubiquitin bound to the compact conformation of USP5 only. PMID: 25970461
    6. Cellular isopeptidase T deubiquitinating enzyme disassembles free ubiquitin chains to facilitate KSHV K7 degradation. PMID: 23720098
    7. polyubiquitin chains by USP5 at sites of damage is important for efficient DSB repair PMID: 24454762
    8. Local administration of NO may prevent neointimal hyperplasia by inhibiting isopeptidase T levels and activity in the vasculature, thereby inhibiting the 26S proteasome in vascular smooth muscle cells. PMID: 23375434
    9. Production of the USP5 isoform 2 was strongly correlated with PTBP1 expression in glioblastoma tumor samples and cell lines. PMID: 21976412
    10. USP5 uses multiple zinc fluoride (ZnF)-ubiquitin binding protein (UBP) domains for substrate targeting and core catalytic function. PMID: 22283393
    11. Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome. PMID: 11771738
    12. identification of catalytic site PMID: 12435595
    13. We report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with ubiquitin. This domain is required for optimal catalytic activation of IsoT. PMID: 16564012
    14. p53 is selectively stabilized because the unanchored polyubiquitin that accumulates after USP5 knockdown is able to compete with ubiquitinated p53 but not with Mdm2 for proteasomal recognition PMID: 19098288

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  • 蛋白家族：
    Peptidase C19 family
  • 数据库链接：

    HGNC: 12628

    OMIM: 601447

    KEGG: hsa:8078

    STRING: 9606.ENSP00000229268

    UniGene: Hs.631661