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  4. UPF1 Antibody

UPF1 Antibody

  • 货号：
    CSB-PA245266
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA245266(UPF1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA245266(UPF1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 60 μg, Lane: Raji cells, Primary antibody: CSB-PA245266(UPF1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
  • 其他：

产品详情

  • Uniprot No.：
    Q92900
  • 基因名：
  • 别名：
    ATP dependent helicase RENT1 antibody; ATP-dependent helicase RENT1 antibody; Delta helicase antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF 1 antibody; hUpf1 antibody; KIAA0221 antibody; Nonsense mRNA reducing factor 1 antibody; NORF 1 antibody; NORF1 antibody; pNORF 1 antibody; pNORF1 antibody; Regulator of nonsense transcripts 1 antibody; RENT 1 antibody; RENT1 antibody; RENT1_HUMAN antibody; Smg 2 antibody; Smg 2 homolog nonsense mediated mRNA decay factor antibody; UP Frameshift 1 antibody; Up frameshift mutation 1 homolog (S. cerevisiae) antibody; Up frameshift mutation 1 homolog antibody; Up frameshift suppressor 1 homolog antibody; Up-frameshift suppressor 1 homolog antibody; UPF 1 antibody; UPF 1 regulator of nonsense transcripts homolog antibody; upf1 antibody; UPF1 regulator of nonsense transcripts homolog antibody; UPF1 RNA helicase and ATPase antibody; Yeast Upf1p homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human UPF1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    WB 1:200-1:1000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA hardoring long 3'UTR by inducing the NMD machinery.
  • 基因功能参考文献：
    1. Status epilepticus led to increased protein levels of Up-frameshift suppressor 1 homolog (Upf1) within the mouse hippocampus. Upf1 protein levels were also higher in resected hippocampus from patients with intractable temporal lobe epilepsy. PMID: 28128343
    2. UPF1 is amplified by hyperphosphorylation which reduces stalls in nonsense-mediated RNA decay PMID: 27511142
    3. UPF1 interacts with PNRC2 and that it triggers 5'-3' exonucleolytic decay of reporter transcripts in tethering assays. PMID: 29348139
    4. UPF1 regulates the expression of genes with functions in inflammation and myeloid cell differentiation via hnRNP E2. PMID: 27573788
    5. HTLV-1 Tax protein interacts with the central helicase core domain of UPF1 and freezes it, which leads to nonsense-mediated mRNA decay inhibition. PMID: 29382845
    6. results indicate that UPF1 can dissociate miRNAs from their mRNA targets, making the miRNAs susceptible to Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay. PMID: 28827400
    7. Overexpression of UPF1 inhibited cell proliferation, cell cycle progression, cell migration and invasion, and promoted cell apoptosis in gastric cancer cells. Moreover, the UPF1-mediated inhibition of gastric cancer progression was reversed by overexpression of MALAT1. PMID: 28942451
    8. The nucleic acids processing activities of UPF1 are consistent with its function in mRNA regulation and suggest that roles in DNA replication could also be influenced by base sequence. PMID: 28541562
    9. MARVELD1 substantially inhibits nonsense-mediated RNA decay by decreasing the pioneer round of translation but not steady-state translation, and is an important component of the molecular machinery containing UPF1 and Y14. MARVELD1 promotes the dissociation of SMG1 from UPF1, resulting in the repression of serine phosphorylation of UPF1, and subsequently blocks the recruitment of SMG5. PMID: 23826386
    10. UPF1 acts as an E3 ligase via its RING domain to promote MYOD protein ubiquitination and degradation and repress myogenesis. PMID: 28669802
    11. Upregulation of SNHG6 regulates ZEB1 expression by competitively binding miR-101-3p and interacting with UPF1 in hepatocellular carcinoma, promoting tumor growth and metastasis. PMID: 27702662
    12. Study showed that UPF1 was down-regulated due to promoter hypermethylation in hepatocellular carcinoma tissues which correlated with decreased survival. PMID: 26759305
    13. CARM1 associates with major nonsense-mediated mRNA decay factor UPF1 and promotes its occupancy on premature terminating codon-containing transcripts in spinal muscular atrophy. PMID: 26656492
    14. Upf1 is a RNA helicase essential for nonsense-mediated mRNA decay. Once recruited onto NMD mRNA targets, Upf1 can scan the entire transcript to irreversibly remodel the mRNP, facilitating its degradation by the NMD machinery. PMID: 26138914
    15. Results present evidence for a critical role for Upf1 ATPase activity in nonsense-mediated decay target discrimination, with preferential ATPase-dependent release of Upf1 from non-target mRNAs as part of the underlying mechanism. PMID: 26253027
    16. GR and PNRC2 interact in a ligand-dependent manner to recruit UPF1 for rapid mRNA degradation PMID: 25775514
    17. Depletion of nonsense-mediated mRNA decay pathway components Upf1, Smg5, and Smg7 led to increased levels of viral proteins and and virus release. PMID: 25211080
    18. UPF1 gets recruited to mRNA and becomes phosphorylated before being exported to the cytoplasm as part of the mRNP. PMID: 24762188
    19. SMG6 requires UPF1 and SMG1 for nonsense-mediated mRNA decay. PMID: 25053839
    20. The study demonstrates that SMG5-SMG7 and SMG6 exhibit different and non-overlapping modes of UPF1 recognition, thus pointing at distinguished roles in integrating the complex nonsense-mediated mRNA decay interaction network. PMID: 25013172
    21. 3' UTR-associated UPF1 undergoes regulated phosphorylation on NMD targets, providing a binding platform for mRNA degradative activities PMID: 25184677
    22. Adenosquamous carcinomas frequently harbor somatically acquired mutations in the UPF1 gene which alter RNA splicing and perturb nonsense-mediated RNA decay. PMID: 24859531
    23. This study demonstrated the quantitative regulation of Upf1 and Upf2 proteins by ubiquitin-proteasome system and SMG1. PMID: 24173962
    24. Results show tha MOV10 interacts with UPF1. Moreover, UPF1 binding sites were identified and showed that both MOV10 and UPF1 interact with RNA in close proximity to each other. PMID: 24726324
    25. FXR1 and UPF1 may have a functional role in prostate cancer progression and metastasis. PMID: 23881279
    26. UPF1 is a critical gene expression regulator of 5-lipoxygenase and other proteins in the monocytes. PMID: 23642263
    27. Thus, whereas ectopic MOV10 restricts human immunodeficiency virus type 1 replication, the related UPF1 helicase functions as a cofactor at an early postentry step. PMID: 23785196
    28. UPF1 binds RNA before translation and gets displaced from the coding regions by translating ribosomes. PMID: 23832275
    29. The SQ domain of the human Upf1 helicase, directly interacts with the helicase domain to impede ATP hydrolysis and RNA unwinding. PMID: 23275559
    30. nonsense-mediated mRNA decay involves UPF1 binding along a 3' UTR whether the 3' UTR contains an exon-junction complex. PMID: 23404710
    31. Data indicate a specific impact of hUPF1 on the regulation of complex I genes. PMID: 22889941
    32. the functions of UPF1 in maintaining the stability of telomeres and of the genome PMID: 22156744
    33. Data show that UPF1 also interacts with proteins associated with nuclear RNA degradation and transcription termination PMID: 22817733
    34. UPF1 is an important factor for the RNA quality control system and the regulation of physiological gene expression, and contributes to DNA replication, DNA repair, telomere metabolism, and stabilization of HIV-1 genomic RNA. PMID: 22622014
    35. HTLV-1 Tax binds to UPF1 and causes an increase in the amount of phospho-UPF1. These activities coincidewith an enhanced localization of UPF1 in the P-bodies, in which Tax was also partially detected PMID: 22553336
    36. The heptameric assembly of the UPF complex is built around UPF2, a scaffold protein with a ring structure that closes around the CH domain of UPF1, keeping the helicase region in an accessible and unwinding-competent state. PMID: 22522823
    37. Recruitment of Stau2 alone or in combination with Upf1 differentially affects the fate of mRNAs. PMID: 22087843
    38. UPF1 interacts with TPP1 and telomerase and sustains telomere leading-strand replication PMID: 21829167
    39. Rescue of UPF1 by expression of exogenous UPF1 was found to suppress vascular smooth muscle cell proliferation. PMID: 21749700
    40. hUPF1 rescues the toxicity of FUS/TLS in yeast model, implicating a possible insufficiency in RNA processing or the RNA quality control machinery in the mechanism of FUS/TLS mediated toxicity. PMID: 21541368
    41. Data show that upon binding to Upf2, the regulatory CH domain of Upf1 undergoes a large conformational change, causing the catalytic helicase domain to bind RNA less extensively and triggering its helicase activity. PMID: 21419344
    42. demonstrate that the ATPase activity of the RNA helicase Upf1 allows disassembly of mRNPs undergoing nonsense-mediated mRNA decay PMID: 21145460
    43. A conserved nonsense-mediated mRNA decay event within HNRNPA2B1 that appears to mediate autoregulation of HNRNPA2B1 expression levels, was identified upon UPF1 knockdown . PMID: 20946641
    44. Study uncovers a 2-step mechanism for Upf1-dependent degradation of mRNAs with long 3'UTRs and proposes a model for 3'UTR length surveillance in which equilibrium binding of Upf1 to mRNAs precedes a kinetically distinct commitment to RNA decay. PMID: 21029861
    45. UPF1 binds PTC-containing mRNA more efficiently than the corresponding PTC-free mRNA in a way that is promoted by the UPF1-CBP80 interaction. PMID: 20691628
    46. The s propose that the bipartite mode of UPF2 binding to UPF1 brings the ribosome and the exon junction complex in close proximity by forming a tight complex after an initial weak encounter with either element. PMID: 19556969
    47. inhibition of rent1/hUpf1 expression abrogated both nonsense-mediated mRNA decay and nonsense-mediated altered splicing of nonsense T cell receptor beta transcripts; rent1/hUpf1 enters the nucleus where it may influence early events in mRNA biogenesis PMID: 12228722
    48. Dcp1a and Dcp2 interact with the nonsense-mediated decay factor Upf1 PMID: 12417715
    49. Data show that phosphorylated hUPF1, the human ortholog of UPF1/SMG-2, forms a complex with human orthologs of the Caenorhabditis elegans proteins SMG-5 and SMG-7. PMID: 14636577
    50. regulated degradation of histone mRNAs requires Upf1, a key regulator of the nonsense-mediated decay pathway, and ataxia telangiectasia and Rad3 related, a key regulator of the DNA damage checkpoint pathway activated during replication stress PMID: 16086026

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, P-body. Nucleus.
  • 蛋白家族：
    DNA2/NAM7 helicase family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 9962

    OMIM: 601430

    KEGG: hsa:5976

    STRING: 9606.ENSP00000262803

    UniGene: Hs.515266