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ULK2 Antibody

  • 货号：
    CSB-PA811629LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate
      All lanes: ULK2 antibody at 2.9µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 113 kDa
      Observed band size: 113 kDa
    • IHC image of CSB-PA811629LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA811629LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ULK2 Polyclonal antibody
  • Uniprot No.：
    Q8IYT8
  • 基因名：
  • 别名：
    ATG1B antibody; KIAA0623 antibody; Serine/threonine protein kinase ULK2 antibody; Serine/threonine-protein kinase ULK2 antibody; ULK2 antibody; ULK2_HUMAN antibody; Unc 51 (C. elegans) like kinase 2 antibody; Unc 51 like autophagy activating kinase 2 antibody; Unc 51 like kinase 2 antibody; Unc-51-like kinase 2 antibody; Unc51.2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Serine/threonine-protein kinase ULK2 protein (211-276AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ULK2 Antibody (CSB-PA811629LA01HU)，的标记方式是Non-conjugated。对于ULK2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA811629LB01HU ULK2 Antibody, HRP conjugated ELISA
    FITC CSB-PA811629LC01HU ULK2 Antibody, FITC conjugated
    Biotin CSB-PA811629LD01HU ULK2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
  • 基因功能参考文献：
    1. MiRNA-26b may play a key role in cell growth and death of laryngeal carcinoma through ULK2 and the PTEN/AKT pathway. PMID: 28713931
    2. The serine/threonine kinase ULK2 binds to and phosphorylates CARMA2sh. PMID: 28230860
    3. Knockdown of ULK2 expression significantly induced autophagy, EMT, and cell migration in lung cancer cell line. PMID: 27062295
    4. miR-26b was down-regulated in LNCaP, DU145, C4-2 and PC-3 cells compared to the two normal prostate cells RWPE-1 and WPMY-1 except DU145 cells. This inversely correlates with ULK2 level in the same cell lines. PMID: 26920049
    5. Kapbeta2 interacts with ULK2 through ULK2's putative PY-NLS motif, and facilitates transport from the cytoplasm to the nucleus, depending on its Ser1027 residue phosphorylation by PKA, thereby reducing autophagic activity. PMID: 26052940
    6. Glioblastoma and glioma cell lines had low levels of ULK2 transcripts. ULK2 promoter methylation and transcript levels showed significant negative correlation. PMID: 24923441
    7. First identification and naming of ULK2 as an ortholog of mouse ULK2. PMID: 12471243

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily
  • 数据库链接：

    HGNC: 13480

    OMIM: 608650

    KEGG: hsa:9706

    STRING: 9606.ENSP00000354877

    UniGene: Hs.168762