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TUT1 Antibody

  • 货号：
    CSB-PA046708
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy.
    • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TUT1 Polyclonal antibody
  • Uniprot No.：
    Q9H6E5
  • 基因名：
    TUT1
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from internal of Human TUT1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
  • 基因功能参考文献：
    1. Crystallographic and biochemical studies of TUT1 revealed the molecular mechanism underlying the specific oligo-uridylylation of the 3'-end of U6 snRNA by TUT1. PMID: 28589955
    2. Star-PAP possesses tumor-suppressing activity. PMID: 28151486
    3. Star-PAP-specific polyadenylation sites usage regulates the expression of the eukaryotic translation initiation factor EIF4A1, the tumor suppressor gene PTEN and the long non-coding RNA NEAT1. PMID: 28911096
    4. Star-PAP recognises a unique nucleotide motif on its target mRNA.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase. PMID: 26496945
    5. Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a. PMID: 25142229
    6. Star-PAP controls E6 mRNA polyadenylation and expression and modulates wild-type p53 levels. PMID: 23416977
    7. The human TUT1 nucleotidyl transferase is a global regulator of microRNA abundance. PMID: 23874977
    8. Star-PAP and its regulatory molecules form a signaling nexus at the 3'-end of target mRNAs to control the expression of select group of genes including the ones involved in stress responses. (Review) PMID: 23306079
    9. PIPKIalpha, PI4,5P(2), and PKCdelta regulate Star-PAP control of BIK expression and induction of apoptosis. PMID: 22244330
    10. The data support a model where Star-PAP binds to the pre-mRNA, recruits the CPSF complex to the 3'-end of pre-mRNA and then defines cleavage by CPSF 73 and subsequent polyadenylation of its target mRNAs. PMID: 21102410
    11. PIPKIalpha co-localizes at nuclear speckles and interacts with a newly identified non-canonical poly(A) polymerase, Star-PAP; the activity of Star-PAP can be specifically regulated by PtdIns4,5P2 PMID: 18288197
    12. These data indicate that CKIalpha, PIPKIalpha, and Star-PAP function together to modulate the production of specific Star-PAP messages. PMID: 18305108

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus speckle.
  • 蛋白家族：
    DNA polymerase type-B-like family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 26184

    OMIM: 610641

    KEGG: hsa:64852

    STRING: 9606.ENSP00000308000

    UniGene: Hs.728983