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TOP1MT Antibody

  • 货号：
    CSB-PA294602
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA294602(TOP1MT Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA294602(TOP1MT Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q969P6
  • 基因名：
    TOP1MT
  • 别名：
    TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthetic peptide of Human TOP1MT
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:5-1:20
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
  • 基因功能参考文献：
    1. This report was the first to focus on the role of TOP1MT in tumors. We identified a new mechanism underlying TOP1MT-deficient gastric cancer (GC) metastasis and showed that TOP1MT played an important role in cancer cells. PMID: 28874393
    2. Data indicate that the different coordination geometry achieved by the two transition metal ions has an important role in modulating their efficiency as topoisomerase I inhibitors. PMID: 24172750
    3. DNA topoisomerase I stimulates BLM helicase activity on a nucleolar-relevant RNA:DNA hybrid. PMID: 23261817
    4. Data reported in this paper indicate that hTop IB does not confer to the cells radio-resistance, but the kinetic analysis of both DNA repair rate as well as colonies growth, demonstrates that the cells containing hTop IB show a more efficient rescue from IR injury. PMID: 23422507
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Type IB topoisomerase family
  • 组织特异性：
    Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
  • 数据库链接：

    HGNC: 29787

    OMIM: 606387

    KEGG: hsa:116447

    STRING: 9606.ENSP00000328835

    UniGene: Hs.528574