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TIAM2 Antibody

  • 货号：
    CSB-PA818235LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA818235LA01HU diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of U251 cells with CSB-PA818235LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TIAM2 Polyclonal antibody
  • Uniprot No.：
    Q8IVF5
  • 基因名：
    TIAM2
  • 别名：
    FLJ41865 antibody; OTTHUMP00000017472 antibody; SIF and TIAM1 like exchange factor antibody; SIF and TIAM1-like exchange factor antibody; STEF antibody; T cell lymphoma invasion and metastasis 2 antibody; T lymphoma invasion and metastasis inducing protein 2 antibody; T-lymphoma invasion and metastasis-inducing protein 2 antibody; TIAM 2 antibody; TIAM-2 antibody; Tiam2 antibody; TIAM2_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human T-lymphoma invasion and metastasis-inducing protein 2 protein (1500-1627AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TIAM2 Antibody (CSB-PA818235LA01HU)，的标记方式是Non-conjugated。对于TIAM2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA818235LB01HU TIAM2 Antibody, HRP conjugated ELISA
    FITC CSB-PA818235LC01HU TIAM2 Antibody, FITC conjugated
    Biotin CSB-PA818235LD01HU TIAM2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane. Activates specifically RAC1, but not CDC42 and RHOA.
  • 基因功能参考文献：
    1. The s show that the Rac1 selective guanine nucleotide exchange factor Sif and TIAM1-like Exchange Factor STEF, also known as TIAM2, localises to the nuclear envelope, co-localises with key perinuclear proteins and regulates the activity of perinuclear Rac1. PMID: 29844364
    2. Tiam2/Rac are key components of EphB2 trans-endocytosis and are important for cell repulsion. PMID: 27597758
    3. Results provide evidence that Sp1 positively controls TIAM2S transcription and that Sp1-mediated transcriptional activation is essential for TIAM2S ectopic expression in liver cancer cells. PMID: 26763486
    4. Knockdown of TIAM2 could up-regulate the expression of E-cadherin. PMID: 24377522
    5. TIAM2 expression was undetectable in normal human liver but was induced in all HCC cell lines and in 86% (78/91) of HCC biopsies. PMID: 21469146
    6. stef gene expressed in a stage- and region-specific manner in the mouse brain PMID: 11900975
    7. STEF protein is a guanine nucleotide exchange factor that specifically activates Rac1 in vitro and in cultured cells, which may lead to changes in the actin cytoskeletal network. PMID: 10364228

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  • 亚细胞定位：
    Cytoplasm. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, growth cone. Cell projection, neuron projection. Perikaryon.
  • 蛋白家族：
    TIAM family
  • 组织特异性：
    Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
  • 数据库链接：

    HGNC: 11806

    OMIM: 604709

    KEGG: hsa:26230

    STRING: 9606.ENSP00000327315

    UniGene: Hs.586279