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THEMIS2 Antibody

  • 货号：
    CSB-PA735984LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: THEMIS2 antibody at 4.2µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 73, 30, 57, 14, 58, 51 kDa
      Observed band size: 57 kDa
    • IHC image of CSB-PA735984LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of NIH/3T3 cells with CSB-PA735984LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) THEMIS2 Polyclonal antibody
  • Uniprot No.：
    Q5TEJ8
  • 基因名：
    THEMIS2
  • 别名：
    Basement membrane induced antibody; Chromosome 1 open reading frame 38 antibody; ICB 1 antibody; ICB1 antibody; Induced by contact to basement membrane 1 protein antibody; Protein ICB 1 antibody; Protein ICB-1 antibody; Protein THEMIS2 antibody; THEMIS2 antibody; THMS2_HUMAN antibody; Thymocyte expressed molecule involved in selection protein 2 antibody; Thymocyte-expressed molecule involved in selection protein 2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein THEMIS2 protein (62-177AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，THEMIS2 Antibody (CSB-PA735984LA01HU)，的标记方式是Non-conjugated。对于THEMIS2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA735984LB01HU THEMIS2 Antibody, HRP conjugated ELISA
    FITC CSB-PA735984LC01HU THEMIS2 Antibody, FITC conjugated
    Biotin CSB-PA735984LD01HU THEMIS2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways.
  • 基因功能参考文献：
    1. SNP rs1467465 of the human gene icb-1 might affect susceptibility to ovarian cancer. PMID: 24826199
    2. Loss of icb-1 expression accelerated proliferation of SK-OV-3, OVCAR-3 and OAW-42 cells and led to upregulation of ovarian cancer biomarkers like KLK10 and CLDN16. PMID: 23474491
    3. Data of this study suggest that icb-1 might exert a tumor-suppressor function in breast cancer and that its loss might confer relative resistance of breast cancer cells to apoptotic drugs. PMID: 22565810
    4. Themis2 and Themis1 exhibit the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
    5. These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation. PMID: 21455565
    6. Data show that a highly significant positive correlation of icb-1 transcript levels with c-erbB2 (HER2) expression (p <.0001). PMID: 20569072
    7. Identification of ICB-1 as a new player in endocrine-related cancer. PMID: 19942713
    8. Increased icb-1 mRNA levels could be observed both in monocytic and granulocytic differentiation in maturing HL-60 cells. PMID: 12191572
    9. The novel interferon-gamma inducible gene icb-1 is identified and its effects on the response of ovarian cancer cells to interferon-gamma are described PMID: 16219472
    10. Data suggest that SNP rs1467465 of human gene icb-1 might affect breast cancer susceptibility. PMID: 19308812

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Themis family
  • 组织特异性：
    Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.
  • 数据库链接：

    HGNC: 16839

    OMIM: 617856

    KEGG: hsa:9473

    STRING: 9606.ENSP00000363031

    UniGene: Hs.10649