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TFB1M Antibody

  • 货号：
    CSB-PA023420LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: TFB1M antibody at 4.6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40 kDa
      Observed band size: 40 kDa
    • IHC image of CSB-PA023420LA01HU diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA023420LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA023420LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) TFB1M Polyclonal antibody
  • Uniprot No.：
    Q8WVM0
  • 基因名：
    TFB1M
  • 别名：
    N'-adenosyl(rRNA) dimethyltransferase 1 antibody; CGI75 antibody; Dimethyladenosine transferase 1; mitochondrial antibody; h-mtTFB antibody; h-mtTFB1 antibody; hmtTFB antibody; hmtTFB1 antibody; hTFB1M antibody; Mitochondrial 12S rRNA dimethylase 1 antibody; Mitochondrial dimethyladenosine transferase 1 antibody; Mitochondrial transcription factor B1 antibody; mtTFB1 antibody; S-adenosylmethionine-6-N' antibody; Tfb1m antibody; TFB1M_HUMAN antibody; Transcription factor B1 mitochondrial antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Dimethyladenosine transferase 1, mitochondrial protein (59-194AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，TFB1M Antibody (CSB-PA023420LA01HU)，的标记方式是Non-conjugated。对于TFB1M Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA023420LB01HU TFB1M Antibody, HRP conjugated ELISA
    FITC CSB-PA023420LC01HU TFB1M Antibody, FITC conjugated
    Biotin CSB-PA023420LD01HU TFB1M Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.
  • 基因功能参考文献：
    1. Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes. PMID: 24916378
    2. Deficiency in TFB1M and impaired mitochondrial function contribute to the pathogenesis of type 2 diabetes. PMID: 21195351
    3. The mRNA levels of TFB1M and TFB2M are influenced by endurance training PMID: 19681768
    4. This transcription factor activates transcription of human mitochondrial DNA. PMID: 12068295
    5. Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop PMID: 12496758
    6. TFB1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity PMID: 12897151
    7. TFB1M is a nuclear-encoded modifier gene for phenotypic expression of the deafness-associated homoplasmic A1555G mutation in the mitochondrial 12S rRNA gene. PMID: 15110318
    8. Distinct, but possibly coordinated functions of mtTFB1 and mtTFB2 in mitochondrial gene expression and biogenesis. PMID: 17557812
    9. This study suggested that DNA variants in TFB1M did not contribute to the risk for parkinson disease. PMID: 18980857
    10. determined the variation in the TFAM, TFB1M, and TFB2M genes in cardiac hypertrophy PMID: 19096125
    11. rRNA methyltransferase activity is necessary for induction of mitochondrial biogenesis by TFB1M, but not TFB2M. PMID: 19417006

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  • 相关疾病：
    Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA.
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family, KsgA subfamily
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 17037

    OMIM: 607033

    KEGG: hsa:51106

    STRING: 9606.ENSP00000356134

    UniGene: Hs.279908