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Samhd1 Antibody

  • 货号：
    CSB-PA020691LA01MO
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: Samhd1 antibody at 2µg/ml + 293T whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 76, 75 kDa
      Observed band size: 76 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Samhd1 Polyclonal antibody
  • Uniprot No.：
    Q60710
  • 基因名：
    Samhd1
  • 别名：
    Samhd1 antibody; Mg21 antibody; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody; dNTPase antibody; EC 3.1.5.- antibody; Interferon-gamma-inducible protein Mg11 antibody; SAM domain and HD domain-containing protein 1 antibody; mSAMHD1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Mouse, Human
  • 免疫原：
    Recombinant Mouse Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 protein (426-657AA)
  • 免疫原种属：
    Mus musculus (Mouse)
  • 标记方式：
    Non-conjugated

    本页面中的产品，Samhd1 Antibody (CSB-PA020691LA01MO)，的标记方式是Non-conjugated。对于Samhd1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA020691LB01MO Samhd1 Antibody, HRP conjugated ELISA
    FITC CSB-PA020691LC01MO Samhd1 Antibody, FITC conjugated
    Biotin CSB-PA020691LD01MO Samhd1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication. Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation. Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity. Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation.
  • 基因功能参考文献：
    1. SAMHD1 inhibits NF-kappaB activation by interacting with NF-kappaB1/2 and reducing phosphorylation of the NF-kappaB inhibitory protein IkappaBalpha. SAMHD1 also interacts with the inhibitor-kappaB kinase epsilon (IKKepsilon) and IFN regulatory factor 7 (IRF7), leading to the suppression of the IFN-I induction pathway by reducing IKKepsilon-mediated IRF7 phosphorylation. SAMHD1 down-regulates innate immune responses to ... PMID: 29610295
    2. SAMHD1's restriction of dNTP supply enhances AID's mutagenicity and that the evolution of Ig hypermutation included the repurposing of antiviral mechanisms based on dNTP starvation. PMID: 29669924
    3. SAMHD1 crystal structure delineates the SAMHD1 allosteric activation process that governs SAMHD1 enzymatic activities. PMID: 29379009
    4. provide genetic evidence that cell-autonomous control of lentivirus infection in myeloid cells by SAMHD1 limits virus-induced production of interferons and the induction of co-stimulatory markers PMID: 27477283
    5. These data support a model in which SAMHD1 catalytic activity is regulated through tetramer stabilization by the carboxyl-terminal tail, phosphorylation destabilizing the complexes and inactivating the enzyme. ISF2 may serve to reduce the dNTP pool to very low levels as a means of restricting virus replication. PMID: 27920203
    6. These observations suggest that heterozygous cancer-associated SAMHD1 mutations increase mutation rates in cancer cells. PMID: 27071091
    7. SAMHD1 in the mouse blocks retroviral infection at the level of reverse transcription and is regulated through cell cycle-dependent phosphorylation. PMID: 26667483
    8. phosphorylation of mSAMHD1 at T634 by CDK1/2 negatively regulates its HIV-1 restriction in differentiated cells, but does not affect its murine leukemia virus restriction in dividing cells. PMID: 26580513
    9. SAMHD1 restricts HIV-1 replication and regulates interferon production in mouse myeloid cells. PMID: 24586870
    10. Allosteric regulation by dATP and dTTP works similarly in human and mouse SAMHD1. PMID: 24828500
    11. SAMHD1 decreases dNTP pool in murine cells, restricts retroviral replication and regulates type I IFN production. PMID: 23972988
    12. SAMHD1 can restrict lentiviruses in vivo and that nucleotide starvation is an evolutionarily conserved antiviral mechanism. PMID: 23872947
    13. there are several Vpx residues required for SAMHD1 degradation PMID: 23076149
    14. Vpx targets SAMHD1 for degradation in a viral strategy to control cellular deoxynucleotide levels for efficient replication PMID: 22069334

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    SAMHD1 family
  • 数据库链接：

    KEGG: mmu:56045

    STRING: 10090.ENSMUSP00000059717

    UniGene: Mm.248478