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SNX12 Antibody

  • 货号：
    CSB-PA891787LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate
      All lanes: SNX12 antibody at 2.83µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 20, 19 kDa
      Observed band size: 20 kDa
    • IHC image of CSB-PA891787LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA891787LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating SNX12 in A549 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA891787LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA891787LA01HU (6µg) + A549 whole cell lysate (500µg)
      Lane 3: A549 whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SNX12 Polyclonal antibody
  • Uniprot No.：
    Q9UMY4
  • 基因名：
  • 别名：
    2610001F05Rik antibody; AW045757 antibody; MGC118982 antibody; MGC118983 antibody; RGD1565585 antibody; RP23-356J7.1 antibody; SDP8 antibody; SNX 12 antibody; SNX12 antibody; SNX12_HUMAN antibody; Sorting nexin 12 antibody; Sorting nexin-12 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Sorting nexin-12 protein (2-172AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SNX12 Antibody (CSB-PA891787LA01HU)，的标记方式是Non-conjugated。对于SNX12 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA891787LB01HU SNX12 Antibody, HRP conjugated ELISA
    FITC CSB-PA891787LC01HU SNX12 Antibody, FITC conjugated
    Biotin CSB-PA891787LD01HU SNX12 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:200-1:500
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May be involved in several stages of intracellular trafficking.
  • 基因功能参考文献：
    1. SNX12 plays a key role in intraluminal vesicle formation and in the maturation of a subpopulation of early endosomes into late endosomes, thereby regulating selective endocytic transport of cargo for degradation. PMID: 28705836
    2. SNX12 protein level is dramatically decreased in the brain of Alzheimer's disease patients as compared to that of controls. PMID: 22709416
    3. find that overexpression of SNX12 restores the sorting process in an Hrs knockdown background. Altogether, our data show that despite lower expression level, SNX12 shares redundant functions with SNX3 in the biogenesis of multivesicular endosomes PMID: 22719997
    4. Metallothionein deficient mice have a marked decrease in Snx12 during acute lung injury PMID: 16166738
  • 亚细胞定位：
    Membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Sorting nexin family
  • 数据库链接：

    HGNC: 14976

    OMIM: 300883

    KEGG: hsa:29934

    STRING: 9606.ENSP00000363392

    UniGene: Hs.260750