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SLC12A5 Antibody

  • 货号：
    CSB-PA863965LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA863965LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA863965LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SLC12A5 Polyclonal antibody
  • Uniprot No.：
    Q9H2X9
  • 基因名：
    SLC12A5
  • 别名：
    Electroneutral potassium chloride cotransporter 2 antibody; Electroneutral potassium-chloride cotransporter 2 antibody; Erythroid K Cl cotransporter 2 antibody; Furosemide sensitive K Cl cotransporter antibody; hKCC2 antibody; K-Cl cotransporter 2 antibody; KCC 2 antibody; KCC2 antibody; KIAA1176 antibody; Neuronal K Cl cotransporter antibody; Neuronal K-Cl cotransporter antibody; Potassium Chloride Cotransporter antibody; Potassium chloride transporter 5 antibody; rKCC2 antibody; S12A5 antibody; S12A5_HUMAN antibody; SLC12A5 antibody; Solute carrier family 12 (potassium chloride transporter) member 5 antibody; Solute carrier family 12 member 5 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Solute carrier family 12 member 5 protein (944-1071AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SLC12A5 Antibody (CSB-PA863965LA01HU)，的标记方式是Non-conjugated。对于SLC12A5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA863965LB01HU SLC12A5 Antibody, HRP conjugated ELISA
    FITC CSB-PA863965LC01HU SLC12A5 Antibody, FITC conjugated
    Biotin CSB-PA863965LD01HU SLC12A5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.
  • 基因功能参考文献：
    1. the long-time considered "neuron-specific" KCC2 co-transporter is expressed in pancreatic islet beta-cells where it modulates Ca(2+)-dependent insulin secretion. PMID: 28496181
    2. These data clearly demonstrated that partially disrupted neuronal Cl(-) extrusion, mediated by two types of differentially impaired KCC2 mutant in an individual, causes epilepsy of infancy with migrating focal seizures. PMID: 27436767
    3. Study describes the developmental patterns of cation-chloride cotransporters in the human brain from the fetal stage to senescence. Expression of KCC2 and its functionally associated proteins begins in early fetal period. PMID: 26428952
    4. The s show that APP can physically interact with KCC2, a neuron-specific K(+)-Cl(-) cotransporter that is essential for Cl(-) homeostasis and fast GABAergic inhibition. PMID: 28054918
    5. SLC12A5 promoted the migration and invasion of BUC by enhancing MMP-7 expression. PMID: 28333147
    6. Study shows that the overall expression of potassium-chloride cotransporter-2 is increased in the hippocampi of temporal lobe epilepsy patients. PMID: 26427846
    7. Our network model suggested the loss of KCC2 in a critical number of pyramidal cells increased external potassium and intracellular chloride concentrations leading to seizure-like field potential oscillations. These oscillations included transient discharges leading to ictal-like field events with frequency spectra as in vitro Restoration of KCC2 function suppressed seizure activity and thus may present a useful therapeut PMID: 27852771
    8. The KCC2 exerts specific functions for the maturation of glycinergic synapses in cultured spinal cord neurons. PMID: 26780567
    9. SLC12A5 plays a pivotal oncogenic role in colorectal carcinogenesis; its overexpression is an independent prognostic factor of patients with CRC. PMID: 25947013
    10. the functional deficit of KCC2 may offer an explanation for the delayed onset of Rett symptoms. PMID: 26733678
    11. A KCC2 mutation causes epilepsy of infancy with migrating focal seizures. Decreased KCC2 expression, reduced protein glycosylation and impaired Cl- extrusion contribute to loss of KCC2 activity, impairing synaptic inhibition and promoting excitability. PMID: 26333769
    12. these data provide insight into the mechanism regulating Cl(-) homeostasis in immature neurons, and suggest that WNK1-regulated changes in KCC2 phosphorylation contribute to the developmental excitatory-to-inhibitory GABA sequence. PMID: 26126716
    13. a novel signaling pathway that couples KCC2 to the cytoskeleton and regulates the formation of glutamatergic synapses. PMID: 26056138
    14. These data describe a novel KCC2 variant significantly associated with a human disease and suggest genetically encoded impairment of KCC2 functional regulation may be a risk factor for the development of human IGE. PMID: 24928908
    15. KCC2-R952H from patients with febrile seizures impairs neuronal Cl- extrusion and dendritic spine formation. PMID: 24668262
    16. Functional characterization of mutant SLC12A5 revealed its potential oncogenic effect in colon cancer. PMID: 24699064
    17. Reduced KCC2/NKCC1 ratio in the cerebrospinal fluid of Rett Syndrome patients suggests a disturbed process of GABAergic neuronal maturation and open up a new therapeutic perspective. PMID: 23894354
    18. Disrupted KCC2 activity seems to be involved in neuropathic pain, epilepsy, motor spasticity, stress and schizophrenia. PMID: 23621303
    19. BPA can disrupt Kcc2 gene expression through epigenetic mechanisms PMID: 23440186
    20. Alternate transcripts from KCC2 may participate in the abnormal GABA signaling in the dorsolateral prefrontal cortex associated with schizophrenia PMID: 22496567
    21. Hyperpolarizing GABAergic transmission depends on KCC2 function and membrane potential. PMID: 22082832
    22. KCC2 is widely expressed in several human cancer cell lines and serves to increase cervical tumourigenesis via an ion transport-independent mechanism PMID: 21911617
    23. The Wnk3 protein isoforms have a similar effect on SLC12 cotransporters. NKCC1/2 and NCC were inhibited, even in hypertonicity, while KCCs were activated, even in isotonic conditions. PMID: 21613606
    24. levels of KCC2 mRNA and protein were significantly decreased in the neurons around large abnormal neurons in focal cortical dysplasia PMID: 21256718
    25. analysis of differences in large extracellular loop between the K(+)-Cl(-) cotransporters KCC2 and KCC4 PMID: 20516068
    26. chromosome 20 mapping PMID: 11701957
    27. Expression of hKCC2 cRNA in Xenopus laevis oocytes results in significant Cl(-)-dependent (86)Rb(+) uptake under isotonic conditions PMID: 12106695
    28. human osteoblasts express functional K-Cl cotransporters in their cell membrane that seem to be able to induce the indirect activation of volume-sensitive Cl- channels by KCl through an increase in the intracellular ions, water influx and cell swelling. PMID: 12637262
    29. GABAergic transmission is influenced by the neuronal expression chloride-extruding K(+)-Cl(-) cotransporter KCC2--REVIEW PMID: 15528236
    30. KCC2 expression is not only necessary but is also sufficient for ending the depolarizing period of GABA in developing cortical neurons PMID: 15932617
    31. constitutive K(+)-Cl(-) cotransport mediated by KCC2 is completely independent of serine-threonine phosphatase activity PMID: 16291749
    32. decrease in the spontaneous activity transients was associated with a developmental up-regulation of the neuronal chloride extruder K+-Cl- cotransporter 2 PMID: 16324114
    33. PKC-dependent phosphorylation of KCC2 may play a central role in modulating both the functional expression of this critical transporter in the brain and the strength of synaptic inhibition PMID: 17693402
    34. The novel KCC2a isoform differs from the only previously known KCC2 isoform (now termed KCC2b) by 40 unique N-terminal amino acid residues, including a putative Ste20-related proline alanine-rich kinase-binding site. PMID: 17715129
    35. the two isoforms can be coimmunoprecipitated from the neonatal brain, suggesting the presence of endogenous KCC2a-KCC2b heteromers. PMID: 19307176
    36. membrane rafts render KCC2 inactive and NKCC1 active PMID: 19686239

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  • 相关疾病：
    Epileptic encephalopathy, early infantile, 34 (EIEE34); Epilepsy, idiopathic generalized 14 (EIG14)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC12A transporter family
  • 组织特异性：
    Brain specific. Detected in neuronal cells.
  • 数据库链接：

    HGNC: 13818

    OMIM: 606726

    KEGG: hsa:57468

    STRING: 9606.ENSP00000387694

    UniGene: Hs.21413