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SHPRH Antibody

  • 货号：
    CSB-PA619905LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA619905LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of SH-SY5Y cells with CSB-PA619905LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SHPRH Polyclonal antibody
  • Uniprot No.：
    Q149N8
  • 基因名：
    SHPRH
  • 别名：
    bA545I5.2 antibody; E3 ubiquitin-protein ligase SHPRH antibody; FLJ27258 antibody; FLJ37625 antibody; FLJ45012 antibody; FLJ90837 antibody; histone-linker antibody; KIAA2023 antibody; MGC134886 antibody; PHD and RING finger domain-containing helicase antibody; Shprh antibody; SHPRH_HUMAN antibody; SNF2 antibody; SNF2 histone linker PHD RING helicase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human E3 ubiquitin-protein ligase SHPRH protein (1362-1512AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SHPRH Antibody (CSB-PA619905LA01HU)，的标记方式是Non-conjugated。对于SHPRH Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA619905LB01HU SHPRH Antibody, HRP conjugated ELISA
    FITC CSB-PA619905LC01HU SHPRH Antibody, FITC conjugated
    Biotin CSB-PA619905LD01HU SHPRH Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
  • 基因功能参考文献：
    1. This study suggests that SHPRH could be a link between mTOR-mediated epigenetic regulations and rRNA transcription, while concomitantly affecting genomic integrity. PMID: 29139335
    2. evidence that SHPRH functions in rRNA transcription through its interaction with histone H3 in a mammalian target of rapamycin (mTOR)-dependent manner PMID: 28400511
    3. HLTF and SHPRH suppress mutagenesis in a damage-specific manner, preventing mutations induced by UV rays and methyl methanesulfonate. PMID: 21396873
    4. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    5. SHPRH maps to 6q24 and is a possible candidate for the tumor suppressor gene reported from that region. PMID: 12837266
    6. SHPRH function is an important deterrent to mutagenesis and carcinogenesis in humans PMID: 17108083
    7. We report that a putative tumor suppressor gene, SHPRH, is a human orthologue of yeast RAD5.The yRad5/SHPRH-dependent pathway is a conserved and fundamental DNA repair mechanism that protects the genome from genotoxic stress. PMID: 17130289
    8. HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. PMID: 18719106

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  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 组织特异性：
    Broadly expressed.
  • 数据库链接：

    HGNC: 19336

    OMIM: 608048

    KEGG: hsa:257218

    STRING: 9606.ENSP00000275233

    UniGene: Hs.723297