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S100A8 Antibody

  • 货号：
    CSB-PA020641HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA020641HA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) S100A8 Polyclonal antibody
  • Uniprot No.：
    P05109
  • 基因名：
  • 别名：
    60B8Ag antibody; AI323541 antibody; B8Ag antibody; BEE11 antibody; CAGA antibody; Calgranulin-A antibody; Calprotectin L1L subunit antibody; Calprotectin; included antibody; CFAG antibody; CGLA antibody; Chemotactic cytokine CP-10 antibody; CP-10 antibody; Cystic fibrosis antigen antibody; L1Ag antibody; Leukocyte L1 complex light chain antibody; MA387 antibody; MIF antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; Myeloid-related protein 8 antibody; Neutrophil cytosolic 7 kDa protein antibody; NIF antibody; p8 antibody; Pro-inflammatory S100 cytokine antibody; Protein S100-A8 antibody; S100 calcium binding protein A8 (calgranulin A) antibody; S100 calcium binding protein A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S100A8/S100A9 complex; included antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein S100-A8 protein (1-93AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，S100A8 Antibody (CSB-PA020641HA01HU)，的标记方式是Non-conjugated。对于S100A8 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA020641HB01HU S100A8 Antibody, HRP conjugated ELISA
    FITC CSB-PA020641HC01HU S100A8 Antibody, FITC conjugated
    Biotin CSB-PA020641HD01HU S100A8 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.
  • 基因功能参考文献：
    1. Study found that that the co-stimulation with both advanced glycation end-products and Porphyromonas gingivalis LPS increased S100A8 and S100A9 mRNA expression in gingival epithelial cells. These results demonstrated that the upregulation of calprotectin in gingival epithelial cells may primarily function intracellularly in this tissue and may contribute to the pathological process of periodontitis. PMID: 28771806
    2. Our results showed that S100A8/A9, absent or present at very low level in skin biopsies from healthy subjects, is dramatically upregulated in each epidermal layer from psoriatic patients. PMID: 29605872
    3. The results suggest that the calcium-binding proteins S100A8 and S100A9 in myeloid cells have an immune regulatory effect. PMID: 29933628
    4. Median MRP8/14 levels were higher in patients with Takayasu arteritis. PMID: 29196802
    5. S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID: 29595187
    6. Wide intra- and interindividual differences in calprotectin concentrations in the meconium may reflect intestinal inflammation associated with the fetal adaptation to life outside the uterus. PMID: 28463850
    7. Salivary calprotectin levels were significantly higher in RAS [Recurrent aphthous stomatitis] group (23.72 +/- 4.28 mg/L) compared to the HC [Healthy Controls] (21.59 +/- 4.27 mg/L) (P = 0.013). No significant relationship was found between calprotectin levels and age or gender in both groups (P >0.05). PMID: 29519972
    8. the levels of S100A8 and S100A9 in the sera, and calprotectin levels in the sera and urines of bladder cancer patients, were determined. PMID: 28609200
    9. MRP-8/14 concentration increases in coronary artery blood in association with thrombus formation in acute coronary syndrome. PMID: 28986057
    10. ANGPTL6, S100A8 and S100A9 are overexpressed in skin of psoriasis patients versus zero expression in controls. PMID: 27698489
    11. Elevated breast cancer cell and stromal S1008 protein expression are significant indicators of poorer outcomes in early stage breast cancer patients. Evaluation of S100A8 protein expression may provide additional prognostic information beyond traditional breast cancer prognostic biomarkers. PMID: 28717852
    12. S100A8 was required for laser-induced new collagen synthesis in skin cells. PMID: 29270708
    13. S100A8 methylation has a role in diagnosis and prognosis of hepatocellular carcinoma PMID: 27462864
    14. This study demonstrated that S100A8 was detected in the serums of Alzheimer disease patients PMID: 27911324
    15. Studies indicate S100A8 as the most overexpressed gene, and the cell cycle pathway as the most promising biomarker and therapeutic target for relapsed childhood B-acute lymphoblastic leukemia (B-ALL). PMID: 28183295
    16. Low S100A8 expression is associated with head and neck neoplasms. PMID: 26883112
    17. Report role of fecal calprotectin assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
    18. S100A8 and S100A9 aggravate Coxsackievirus B3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies. PMID: 29158436
    19. Data suggest that fecal calprotectin may be a potential biomarker to identify patients with ankylosing spondylitis (AS) at risk of developing inflammatory bowel disease (IBD). PMID: 28148281
    20. Elevated S100A8 and S100A9 gene expression in SP-infected HMEECs and in the middle ear mucosa of OM, minor co-localized with neutrophil markers suggests that middle ear epithelial cell secretion of S100A8 and S100A9 may play a role in the pathogenesis of recurrent and chronic OM PMID: 28964306
    21. results of this study support an additional role of calprotectin in assessing inflammatory activity in patients with RA PMID: 28832684
    22. Data show that E3 ubiquitin ligase HRD1 (HRD1) decreased the protein level of S100A8 through ubiquitination. PMID: 28423597
    23. High S100A8 expression is associated with glioblastoma. PMID: 27006175
    24. This study implicates S100A8 and S100A9 as important mediators of tumor cell aggressiveness, and highlights the therapeutic potential of S100A8 and S100A9 for interference of metastasis. PMID: 27086923
    25. Data indicate a statistical correlation between fecal calprotectin (FC) and gastrointestinal graft-versus-host disease (GvHD). PMID: 28293713
    26. Perinatal alarmins S100A8 and S100A9 specifically altered MyD88-dependent proinflammatory gene programs. S100 programming prevented hyperinflammatory responses without impairing pathogen defense. TRIF-adaptor-dependent regulatory genes remained unaffected by perinatal S100 programming and responded strongly to lipopolysaccharide, but were barely expressed. PMID: 28459433
    27. Expression of S100A8, S100A9 and S100A12 is modulated by eicosapentaenoic acid and docosahexaenoic acid during Inflammation in adipose tissue and mononuclear cells. PMID: 28125622
    28. High S100A8 expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
    29. Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
    30. S100A8 and S100A9 proteins in concentrations secreted by CD34(-)/CD31(+) circulating angiogenic cells (CACs) with impaired function reduce endothelial cell capillary-like network formation. These effects appear to be mediated by Toll-like receptor 4 and are absent with S100A8 and S100A9 in concentrations secreted by healthy CD34(-)/CD31(+) CACs. PMID: 27793853
    31. MRP8 elevated serum levels in patients with Behcet's disease indicate MRP8 role in the pathogenetic mechanisms of Behcet's disease. PMID: 25471894
    32. High baseline serum MRP8/14 levels are associated with lower survival in Chinese peritoneal dialysis patients. PMID: 22699162
    33. In acute coronary syndromes, MRP8 and MRP8/14 complex are specific ligands of TLR4, which induce the release of TNFalpha and probably other pro-inflammatory agents from monocytes PMID: 21782178
    34. study revealed a novel role of MRP8/14 in the autophagy-mediated elimination of intracellular BCG by promoting reactive oxygen species generation PMID: 25312864
    35. MRP8 has a crucial role in stimulating IL-6 expression by rheumatoid arthritis fibroblast-like synoviocytes. PMID: 23619188
    36. decreased pretreatment levels of myeloid-related protein-8/14 and C-reactive protein may have a role in poor adherence to treatment with tumor necrosis factor inhibitors in juvenile idiopathic arthritis PMID: 27562034
    37. MRP8/14 can activate cells by binding to TLR4, leading to the release of proinflammatory cytokines. These mediators can lead to increased disease activity in enthesitis-related arthritis. PMID: 24231443
    38. Fibronectin adhesive capacity and integrin expression of monocytes of type 1 and type 2 diabetic patients is related to the subjects' serum levels of MRP-8. PMID: 15277376
    39. Excessive release of cytotoxic MRP8/MRP14 by activated phagocytes might therefore present an important molecular pathomechanism contributing to endothelial damage during vasculitis and other inflammatory diseases PMID: 17095618
    40. MRP8 expression is related to the pathological stage of laryngeal squamous cell carcinoma. PMID: 22256739
    41. MRP8/14 in ankylosing spondylitis is associated with peripheral arthritis and active disease PMID: 27738838
    42. Circulating MRP-8/14 is associated with TX-dependent platelet activation in ACS, even during low-dose aspirin treatment, suggesting a contribution of residual TX to MRP-8/14 shedding, which may further amplify platelet activation. PMID: 25037196
    43. Phosphorylation inhibits tubulin polymerization in microtubules. PMID: 15331440
    44. Data suggest that tissue infiltrating macrophages secreting MRP8/14 may contribute to myositis, by driving the local production of cytokines directly from muscle. PMID: 24286299
    45. calprotectin induces IL-6 and MCP-1 production in Human Gingival Fibroblasts via TLR4 signaling that involves MAPK and NF-kappaB, resulting in the progression of periodontitis. PMID: 27925202
    46. Findings suggest that S100A8/A9 may be involved in the inflammatory response with induction of proinflammatory cytokines and may serve as a clinicopathological marker for disease activity in AOSD. PMID: 27537874
    47. Peripheral blood levels of S100A8 and TRAF6 in SAE patients were elevated and might be related to the severity of SAE and predict the outcome of SAE. PMID: 27411454
    48. Low S100A8/A9 levels may contribute to poor wound healing in colonized chronic wounds with striking difference between diabetic foot ulcers and venous leg ulcers. PMID: 27084691
    49. Suggest that, in individuals with lichen planus, S100A8 may exert distinct immunomodulatory and cytotoxicity functions. PMID: 26632637
    50. Serum calprotectin was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. It was higher in seropositive than in seronegative patients. PMID: 26767827

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  • 亚细胞定位：
    Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes
  • 数据库链接：

    HGNC: 10498

    OMIM: 123885

    KEGG: hsa:6279

    STRING: 9606.ENSP00000357721

    UniGene: Hs.416073