Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. RTF1 Antibody

RTF1 Antibody

  • 货号：
    CSB-PA821675HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA821675HA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA821675HA01HU at dilution of 1:100
    • Immunofluorescent analysis of Hela cells using CSB-PA821675HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
      All lanes: RTF1 antibody at 3.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 81 kDa
      Observed band size: 81 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RTF1 Polyclonal antibody
  • Uniprot No.：
    Q92541
  • 基因名：
    RTF1
  • 别名：
    CDG1N antibody; GTL 7 antibody; GTL7 antibody; KIAA0252 antibody; Ortholog of mouse gene trap locus 7 antibody; RNA polymerase associated protein RTF1 homolog antibody; RNA polymerase-associated protein RTF1 homolog antibody; RTF 1 antibody; RTF1 antibody; Rtf1 Paf1/RNA polymerase II complex component homolog antibody; RTF1_HUMAN antibody; rtf1L antibody; YGL244W antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human RNA polymerase-associated protein RTF1 homolog protein (620-670AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RTF1 Antibody (CSB-PA821675HA01HU)，的标记方式是Non-conjugated。对于RTF1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA821675HB01HU RTF1 Antibody, HRP conjugated ELISA
    FITC CSB-PA821675HC01HU RTF1 Antibody, FITC conjugated
    Biotin CSB-PA821675HD01HU RTF1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:2000-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.
  • 基因功能参考文献：
    1. Human Rtf1 as a transcription elongation factor. PMID: 26217014
    2. Data indicate that the Plus3 domain of the Rtf1 subunit mediates Paf1C recruitment to genes by binding a repeating domain within the phosphorylated elongation factor Spt5. PMID: 24101474
  • 亚细胞定位：
    Nucleus, nucleoplasm.
  • 数据库链接：

    HGNC: 28996

    OMIM: 611633

    KEGG: hsa:23168

    STRING: 9606.ENSP00000374280

    UniGene: Hs.511096