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RRAGA Antibody

  • 货号：
    CSB-PA03465A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA03465A0Rb at dilution of 1:100
    • Immunofluorescence staining of A549 cells with CSB-PA03465A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RRAGA Polyclonal antibody
  • Uniprot No.：
    Q7L523
  • 基因名：
  • 别名：
    Adenovirus E3 14.7 kDa-interacting protein 1 antibody; FIP-1 antibody; Rag A antibody; RagA antibody; Ras-related GTP-binding protein A antibody; RRAGA antibody; RRAGA_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ras-related GTP-binding protein A protein (1-247AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RRAGA Antibody (CSB-PA03465A0Rb)，的标记方式是Non-conjugated。对于RRAGA Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA03465B0Rb RRAGA Antibody, HRP conjugated ELISA
    FITC CSB-PA03465C0Rb RRAGA Antibody, FITC conjugated
    Biotin CSB-PA03465D0Rb RRAGA Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.; (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
  • 基因功能参考文献：
    1. cryo-electron microscopy to solve structures of GATOR1 and GATOR1-RagA GTPase complexes; GATOR1 adopts an extended architecture with a cavity in the middle; NPRL2 links DEPDC5 and NPRL3, and DEPDC5 contacts the RagA GTPase heterodimer PMID: 29590090
    2. Full-length RagA(GTP):RagC(GDP) dimer binds to regulator to activate mTORC1. PMID: 29107538
    3. NUDT2 is a novel complex formation enhancing factor regulating mTORC1-Rag GTPase signaling that is crucial for cell growth control. PMID: 28089905
    4. The dynamic response of mTORC1 requires intersubunit communication by the Rag GTPases, providing a rationale for why they exist as a dimer and revealing a distinct mode of control for a GTP-binding protein. PMID: 29056322
    5. The data indicate that the RRAGA mutations, associated with autosomal dominant cataracts, play a role in the disease by acting through disruption of mTORC1 signaling. PMID: 27294265
    6. Data suggest that both microtubule-associated DYNLT (dynein light chain Tctex-type 1) and cytoplasmic DYNLT (dynein 1 intermediate chain 2 DYNC1LI2) are equally able to bind to small GTPases Rab3D (Rab3d GTPase) and RagA (Ras-related GTP binding A). PMID: 26227614
    7. Data suugest that S-phase kinase-associated protein 2 (Skp2)-mediated Ras-related GTP-binding protein RagA ubiquitination recruits GATOR1 to restrict mTORC1 signaling upon sustained amino acid stimulation. PMID: 26051179
    8. A mechanism for regulation of mTORC1 signaling by RNF152-mediated K63-linked polyubiquitination of RagA. PMID: 25936802

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Lysosome.
  • 蛋白家族：
    GTR/RAG GTP-binding protein family
  • 组织特异性：
    Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
  • 数据库链接：

    HGNC: 16963

    OMIM: 612194

    KEGG: hsa:10670

    STRING: 9606.ENSP00000369899

    UniGene: Hs.743260