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RNF135 Antibody

  • 货号：
    CSB-PA811599ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: RNF135 antibody at 5.88µg/ml
      Lane 1: 293T whole cell lysate
      Lane 2: Jurkat whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 48, 23, 31 kDa
      Observed band size: 48 kDa
    • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA811599ESR2HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RNF135 Polyclonal antibody
  • Uniprot No.：
    Q8IUD6
  • 基因名：
    RNF135
  • 别名：
    E3 ubiquitin-protein ligase RNF135 antibody; L13 antibody; MGC13061 antibody; MMFD antibody; REUL antibody; RIG-I E3 ubiquitin ligase antibody; RING finger protein 135 antibody; Riplet antibody; RN135_HUMAN antibody; Rnf135 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human E3 ubiquitin-protein ligase RNF135 protein (339-432AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    E2-dependent E3 ubiquitin-protein ligase that functions as a RIG-I/DDX58 coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIG-I/DDX58 filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway.
  • 基因功能参考文献：
    1. Together with Riplet, Ube2D3 promotes covalent conjugation of polyubiquitin chains to RIG-I, while Ube2N preferentially facilitates production of unanchored chains. In the presence of these chains, RIG-I induces MAVS aggregation directly on the mitochondria. Data thus reveal two essential mechanisms underlying the activation of RIG-I and MAVS for triggering innate immune signaling in response to viral infection in cells. PMID: 28469175
    2. In-depth analysis of population-differentiated regions indicated that the coding gene, RNF135, represented a trans-regulation hotspot via cis-regulation by the population-specific variants in the region of selective sweep. PMID: 27992444
    3. findings demonstrate the biological effects of RNF135 in glioblastoma cell proliferation, migration and cell cycle, and its role in the progression of glioblastoma may be associated with the ERK signal transduction pathway. PMID: 26856755
    4. Data showed that the presence of the RNF135 K115 variant in the genotype of patients with autism was statistically significant. PMID: 26368817
    5. Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein. PMID: 23950712
    6. REUL is an E3 ubiquitin ligase of RIG-I and specifically stimulates RIG-I-mediated innate antiviral activity PMID: 19484123
    7. These data identify RNF135 as causative of a new overgrowth syndrome and demonstrate that RNF135 haploinsufficiency contributes to the phenotype of NF1 microdeletion cases. PMID: 17632510
    8. identify an alternative factor, Riplet/RNF135, that promotes RIG-I activation independent of TRIM25 PMID: 19017631
    9. RNF135 mutations are not present in patients with Sotos syndrome-like features. PMID: 19291764

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  • 相关疾病：
    Macrocephaly, macrosomia, facial dysmorphism syndrome (MMFD)
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Stress granule.
  • 组织特异性：
    Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung.
  • 数据库链接：

    HGNC: 21158

    OMIM: 611358

    KEGG: hsa:84282

    STRING: 9606.ENSP00000328340

    UniGene: Hs.29874