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RFXANK Antibody

  • 货号：
    CSB-PA019617LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate
      All lanes: RFXANK antibody at 6.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 29, 25, 26 kDa
      Observed band size: 29 kDa
    • IHC image of CSB-PA019617LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of PC-3 cells with CSB-PA019617LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RFXANK Polyclonal antibody
  • Uniprot No.：
    O14593
  • 基因名：
  • 别名：
    ANKRA1 antibody; Ankyrin repeat containing regulatory factor X associated protein antibody; Ankyrin repeat family A protein 1 antibody; BLS antibody; DNA-binding protein RFXANK antibody; F14150_1 antibody; MGC138628 antibody; Regulatory factor X associated ankyrin containing protein antibody; Regulatory factor X subunit B antibody; Regulatory factor X, ankyrin repeat containing antibody; Regulatory factor X-associated ankyrin-containing protein antibody; RFX Bdelta4 antibody; RFX-B antibody; RFXANK antibody; RFXB antibody; RFXB delta 4 antibody; RFXK_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA-binding protein RFXANK protein (3-84AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RFXANK Antibody (CSB-PA019617LA01HU)，的标记方式是Non-conjugated。对于RFXANK Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA019617LB01HU RFXANK Antibody, HRP conjugated ELISA
    FITC CSB-PA019617LC01HU RFXANK Antibody, FITC conjugated
    Biotin CSB-PA019617LD01HU RFXANK Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. May also potentiate the activation of RAF1.; Isoform 2 is not involved in the positive regulation of MHC class II genes.
  • 基因功能参考文献：
    1. mutation results in Bare Lymphocyte Syndrome, Type 2 in a child of Mexican descent PMID: 26634365
    2. genetic, clinical, and immunologic features of 35 patients from 30 unrelated kindreds from North Africa sharing the same RFXANK founder mutation, a 26-bp deletion called I5E6-25_I5E6 + 1) PMID: 21908431
    3. Findings confirm the association between the high frequency of the combined immunodeficiency and the defect in MHC class II expression and provides strong evidence for a founder effect of the 752delG26 mutation in the North African population. PMID: 20414676
    4. Low levels of RFX-B are found in macrophages of colorectal cancer patients, partly explaining immunodeficiency in cancer. PMID: 11836625
    5. in vivo effects of mutations on the expression of the RFXANK RNA and protein PMID: 12618906
    6. ANKRA, RFXANK, and CIITA are novel targets of class IIa HDACs which may deacetylases play a role in regulating MHCII expression PMID: 15964851
    7. analysis of RFXANK domains and function PMID: 16166641
    8. RFXAP and RFXB have roles in relieving autoinhibition of RFX5 PMID: 18723135

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  • 相关疾病：
    Bare lymphocyte syndrome 2 (BLS2)
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 9987

    OMIM: 209920

    KEGG: hsa:8625

    STRING: 9606.ENSP00000305071

    UniGene: Hs.153629