RFX2 Antibody
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货号:CSB-PA019611LA01HU
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规格:¥440
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促销:
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图片:
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IHC image of CSB-PA019611LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA019611LA01HU diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of MCF-7 cells with CSB-PA019611LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
产品详情
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产品名称:Rabbit anti-Homo sapiens (Human) RFX2 Polyclonal antibody
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Uniprot No.:P48378
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基因名:RFX2
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别名:DNA-binding protein RFX2 antibody; HLA class II regulatory factor RFX2 antibody; regulatory factor X 2 (influences HLA class II expression) antibody; Regulatory factor X 2 antibody; regulatory factor X2 antibody; RFX2 antibody; RFX2_HUMAN antibody; trans acting regulatory factor 2 antibody
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宿主:Rabbit
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反应种属:Human
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免疫原:Recombinant Human DNA-binding protein RFX2 protein (1-130AA)
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
本页面中的产品,RFX2 Antibody (CSB-PA019611LA01HU),的标记方式是Non-conjugated。对于RFX2 Antibody,我们还提供其他标记。见下表:
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克隆类型:Polyclonal
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抗体亚型:IgG
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纯化方式:>95%, Protein G purified
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, IHC, IF
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推荐稀释比:
Application Recommended Dilution IHC 1:500-1:1000 IF 1:200-1:500 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相关产品
靶点详情
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功能:Transcription factor that acts as a key regulator of spermatogenesis. Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function. Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters. Probably activates transcription of the testis-specific histone gene H1-6.
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基因功能参考文献:
- Rfx2 coordinates multiple, distinct gene expression programs in multi-ciliated epithelial cells, regulating genes that control cell movement, ciliogenesis, and cilia function. PMID: 24424412
- We show binding of RFX proteins to an evolutionarily conserved X-box in the ALMS1 proximal promoter and present evidence that these proteins are responsible for ALMS1 transcription during growth arrest PMID: 20381594
- novel role for the RFX family of transcription factors as modulators of Ras signalling in epithelial cells. PMID: 15024578
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亚细胞定位:Nucleus. Cytoplasm.
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蛋白家族:RFX family
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数据库链接:
HGNC: 9983
OMIM: 142765
KEGG: hsa:5990
STRING: 9606.ENSP00000306335
UniGene: Hs.465709
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