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RFX1 Antibody

  • 货号：
    CSB-PA019610LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate
      All lanes: RFX1 antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 105 kDa
      Observed band size: 105 kDa
    • IHC image of CSB-PA019610LA01HU diluted at 1:700 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA019610LA01HU diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RFX1 Polyclonal antibody
  • Uniprot No.：
    P22670
  • 基因名：
    RFX1
  • 别名：
    AI047719 antibody; AI385641 antibody; EF-C antibody; EFC antibody; Enhancer factor C antibody; MHC class II regulatory factor RFX antibody; MHC class II regulatory factor RFX1 antibody; Regulatory factor X 1 antibody; Regulatory factor X, 1 (influences HLA class II expression) antibody; Regulatory factor X1 antibody; RFX antibody; RFX-1 antibody; Rfx1 antibody; RFX1_HUMAN antibody; Trans-acting regulatory factor 1 antibody; Transcription factor RFX1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human MHC class II regulatory factor RFX1 protein (36-133AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RFX1 Antibody (CSB-PA019610LA01HU)，的标记方式是Non-conjugated。对于RFX1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA019610LB01HU RFX1 Antibody, HRP conjugated ELISA
    FITC CSB-PA019610LC01HU RFX1 Antibody, FITC conjugated
    Biotin CSB-PA019610LD01HU RFX1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
  • 基因功能参考文献：
    1. RFX1 interacts with the D sequence of adeno-associated virus inverted terminal repeat and regulates AAV transduction. PMID: 29317724
    2. RFX1 expression was significantly lower in HCC tissues compared to the corresponding non-tumor tissues. In vitro studies suggested that knocking down RFX1 facilitated HCC cell invasion, while overexpression of RFX1 reduced the invasion of HCC cells. RFX1 regulated expression of some epithelial-mesenchymal transition markers. PMID: 29764705
    3. Findings indicate that RFX1 functions downstream of STAT3 and phosphorylated STAT3 can inhibit RFX1 expression and suggest a unique role for RFX1 in Th17-related autoimmune diseases. PMID: 29422534
    4. Results show that reduced miRNA-124 improved microglia activation and up-regulated its downstream target RFX1, indicating that RFX1, as one of the target transcripts, facilitates microglial activation by miR-124 regulation; identified the binding site through which RFX1 directly regulates ApoE, may explain the missing link between decreased miRNA-124 and ineffective Abeta uptaking during aging. PMID: 28003160
    5. induction of ciliogenesis increases the expression of RFX and dyslexia candidate genes PMID: 27451412
    6. Data show that transcription factor regulatory factor X 1 (RFX1) protein expression can be tightly regulated by polyubiquitination-mediated proteosomal degradation via STIP1 homology and U-box containing protein 1 (STUB1). PMID: 27283392
    7. therapeutic targets for childhood severe asthmatics identified thru DNA microarray PMID: 25979195
    8. Data indicate that regulatory factor X transcription factor RFX-1 regulates SC-2001-mediated SHP-1 Phosphatase transcription in hepatocellular carcinoma cells. PMID: 24952874
    9. regulatory factor 1 directly regulates CD44 expression in glioblastoma PMID: 24526308
    10. Taken together, this study suggests ciliogenic RFX transcription factors regulate FGF-1B promoter activity and the maintenance of F1BGFP(+) NSPCs and GBM-SCs. PMID: 22415835
    11. RFX1 reduces cell proliferation through inhibiting the TGFbeta2-ERK signaling pathway. RFX1 blocks TGFbeta2 expression through its direct action on TGFbeta2 transcription. PMID: 22582395
    12. NLRC5-mediated histocompatibiility class I gene induction requires the W/S and X1, X2 cis-regulatory elements. PMID: 22490869
    13. RFX1 recruits SUV39H1 to the promoter regions of the CD11a and CD70 genes in CD4(+) T cells, thereby regulating local H3K9 tri-methylation levels. PMID: 21192791
    14. regulates autoimmunity via epigenetic modifications in T cells of systemic lupus erythematosus patients PMID: 20223637
    15. We show binding of RFX proteins to an evolutionarily conserved X-box in the ALMS1 proximal promoter and present evidence that these proteins are responsible for ALMS1 transc PMID: 20381594
    16. RFX1 may negatively regulate the self-renewal of GBM-SCs through modulating FGF-1B and FGF1 expression levels by binding the 18-bp cis-elements of the F1B promoter. PMID: 20189986
    17. Data show that two regulatory factor for X box (RFX1 and 3) binding sites in exon1 of both the mouse and human microtubule-associated protein (MAP1A) gene are important for effective transcriptional repression in non-neuronal cells. PMID: 12411430
    18. Disruption of the RFX site within 263P blunts repressor activity in transfected GC cells; however, repression is only abolished when both PSE-A/RFX and PSE-B/NF-1 sites are mutated. PMID: 12624117
    19. novel role for the RFX family of transcription factors as modulators of Ras signalling in epithelial cells PMID: 15024578
    20. RFX1 gene may be epigenetically silenced in human gliomas and involved in glioma tumorigenesis. PMID: 15334059
    21. Six novel S'-Y' regulatory modules have been identified that are controlled by the human major histocompatibility class II-specific regulatory factor RFX complex. PMID: 15528357
    22. evidence for a common mechanism for Crt1 and Rfx1 expression and for the conservation of their mode of action in response to a DNA replication block in yeasts and humans PMID: 16287876
    23. The binding of alpha Adducin to RFX-I and their nuclear co-localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX-I. PMID: 16289097
    24. results demonstrate that RFX1 and RFX5 differentially interact with class I HDACs underlying the different pathways when repressing collagen synthesis PMID: 16464847
    25. RFX1 mediates the immediate early response of the Id2 gene by serum stimulation and suggest that the function of RFX1 is regulated intramolecularly in its suppression in growth-arrested cells. PMID: 17630394
    26. RFX1 specifically bind to promoter region and negatively regulate the transcription of the human PNRC gene. PMID: 19334528
    27. RFX and/or enhanceosome assembly plays a key role independent of transcriptional coactivator CIITA in protecting major histocompatibility class II antigen promoters against DNA methylation. PMID: 19620312

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RFX family
  • 数据库链接：

    HGNC: 9982

    OMIM: 600006

    KEGG: hsa:5989

    STRING: 9606.ENSP00000254325

    UniGene: Hs.655215