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REV3L Antibody

  • 货号：
    CSB-PA040209
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    O60673
  • 基因名：
    REV3L
  • 别名：
    REV3L antibody; POLZ antibody; REV3 antibody; DNA polymerase zeta catalytic subunit antibody; EC 2.7.7.7 antibody; Protein reversionless 3-like antibody; REV3-like antibody; hREV3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human DNA pol ζ.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.
  • 基因功能参考文献：
    1. Following challenge with AFB1, survival of mouse cells deficient in pol zeta (Rev3L(-/-)) was significantly reduced relative to Rev3L(+/-) cells or Rev3L(-/-) cells complemented through expression of the wild-type human REV3L PMID: 27849610
    2. These results demonstrate a previously unrecognized relationship between p53 and REV3L in cancer cell metabolism and may lead to improvements in chemotherapy treatment plans that reduce cisplatin resistance in lung cancer. PMID: 29307819
    3. REV3/ATR knockdown enhances the cytotoxicity of cisplatin in non-small cell lung cells. PMID: 28075014
    4. The data directly show that, in the human genome, DNA Pol-eta and Rev1 bypass cyclobutane pyrimidine dimers and 6-4PP at replication forks, while only 6-4PP are also tolerated by a Rev3L-dependent gap-filling mechanism, independent of S phase. PMID: 27095204
    5. The results indicate that human DNA polymerase zeta plays important roles in induction of mutations, clastogenicity and in cellular survival of the damaged human cells. PMID: 27338670
    6. loss-of-function of REV3L dramatically enhanced the sensitivity of SCCHN cells to dacomitinib by the loss of both translesion synthesis and homologous recombination pathways. PMID: 26790612
    7. REV3L plays an important role in esophageal squamous cell carcinoma (ESCC) progression and chemoresistance, and is a potential diagnostic marker and therapeutic target for ESCC PMID: 26752104
    8. REV3 functions in mammalian mitochondria and that mitochondrial REV3 is associated with the tumorigenic potential of cells. PMID: 26462070
    9. Taken together, we demonstrated that inhibition of REV3L sensitized lung cancer H1299 cells to cisplatin treatment PMID: 26165320
    10. finding that PLXND1 and REV3L mutations are responsible for a proportion of MBS patients suggests that de novo mutations in other genes might account for other MBS patients PMID: 26068067
    11. data suggest that REV3L plays an important role in regulating cervical cancer cellular response to cisplatin PMID: 25781640
    12. Rev3, the catalytic subunit of polymerase zeta (Pol zeta), is involved in DNA replication under conditions of replication stress caused by deoxyribonucleotide shortage and/or imbalance in human lung adenocarcinoma cells. PMID: 25113059
    13. Single nucleotide polymorphisms in RBPJ, IL1R1, REV3L, TRAF3IP2, IRF1 and ICOS showed association with rheumatoid arthritis in black South Africans. PMID: 25014791
    14. Our results suggest for the first time that REV1 and REV3L SNPs might serve as potential predictive markers of outcome of cisplatin-based chemotherapy PMID: 24956248
    15. Human Pol eta inserts a nucleotide opposite the lesion, followed by Pol zeta extending the DNA primer; thus, the two complement each other to fully bypass the cisplatin cross-link. PMID: 24449906
    16. DNA polymerase zeta is a predictor of poor prognosis for cervical cancer patients who are resistant to chemoradiation. PMID: 23456618
    17. A long-term depletion of Rev3 in cultured human cells results in massive genomic instability and severe cell cycle arrest. PMID: 23303771
    18. REV3L rs465646 variant modifies lung cancer susceptibility in Chinese Han population by affecting miRNA-mediated gene regulation PMID: 22349819
    19. analysis of the crystal structure of the ternary complex composed of the C-terminal domain of human REV1, REV7, and a REV3 fragment PMID: 22859296
    20. ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment has been crystallized. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 74.7, c = 124.5 A PMID: 22869133
    21. our data suggest a significant role of genetic variation in the polymerase zeta subunit genes regarding the development and progression of BC. PMID: 21455670
    22. DNA polymerase zeta transgene participates directly in immunoglobulin hypermutation. PMID: 22547703
    23. REV7 subunit of pol zeta mediated the interaction between REV3 and the REV1 C terminus. PMID: 22303021
    24. The REV1/Polzeta complex maintains genomic stability by directly participating in DNA double-stranded break repair. PMID: 21926160
    25. Findings indicate that depletion of REV3 not only can amend cisplatin-based cancer therapy but also can be applied for susceptible cancers as a potential monotherapy. PMID: 22028621
    26. Data suggest that REV3 plays an important role in different cellular growth periods and physiological conditions. PMID: 20466635
    27. function of REV7 as an adapter protein to recruit Polzeta to a DNA lesion site PMID: 20164194
    28. show the first crystal structure of REV7 in complex with a fragment of REV3 polymerase (residues 1847-1898) and reveal the mechanism underlying REV7-REV3 interaction PMID: 20164194
    29. REV1 and Polzeta facilitate repair of interstrand cross-links independently of PCNA monoubiquitination and Poleta, whereas RAD18 plus Poleta, REV1, and Polzeta are all necessary for replicative bypass of cisplatin intrastrand DNA cross-links. PMID: 20028736
    30. To clarify the structural basis of the interaction between REV7 and REV3, REV7 was crystallized in complex with a REV3 fragment. PMID: 20054135
    31. REV3L Overexpression confers chemoresistance to cisplatin in gliomas. PMID: 19289490
    32. plays a critical role in the induction of mutations PMID: 12459444
    33. REV3 gene plays a role not only in lesion-targeted DNA mutagenesis, but also in mutagenesis on undamaged DNA templates that is called untargeted mutation. PMID: 12717825
    34. downregulation of rev3 might have occurred early during tumorigenesis. PMID: 18622427
    35. The study revealed that suppression of REV3 delayed spontaneous S phase progression and suppression of REV3 limits spontaneous and DNA damage induced mutagenesis. PMID: 18779149
    36. DNA polymerase zeta cooperates with polymerases kappa and iota in translesion DNA synthesis across pyrimidine photodimers in cells from xeroderma pigmentosum variant patients. PMID: 19564618

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 9968

    OMIM: 602776

    KEGG: hsa:5980

    STRING: 9606.ENSP00000351697

    UniGene: Hs.232021