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  4. REV1 Antibody

REV1 Antibody

  • 货号：
    CSB-PA883372LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA883372LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA883372LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) REV1 Polyclonal antibody
  • Uniprot No.：
    Q9UBZ9
  • 基因名：
    REV1
  • 别名：
    AIBP 80 antibody; AIBP80 antibody; Alpha integrin binding protein 80 antibody; Alpha integrin-binding protein 80 antibody; DNA repair protein REV 1 antibody; DNA repair protein REV1 antibody; FLJ21523 antibody; MGC163283 antibody; MGC26225 antibody; REV 1 (yeast homolog) like antibody; REV 1 antibody; REV 1 homolog antibody; REV 1 like antibody; REV 1 protein antibody; REV 1L antibody; REV1 (yeast homolog) like antibody; REV1 antibody; REV1 homolog (S. cerevisiae) antibody; REV1 homolog antibody; REV1 like (yeast) antibody; REV1 like antibody; Rev1 like terminal deoxycytidyl transferase antibody; REV1 protein antibody; Rev1-like terminal deoxycytidyl transferase antibody; REV1_HUMAN antibody; REV1L antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA repair protein REV1 protein (1035-1160AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，REV1 Antibody (CSB-PA883372LA01HU)，的标记方式是Non-conjugated。对于REV1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA883372LB01HU REV1 Antibody, HRP conjugated ELISA
    FITC CSB-PA883372LC01HU REV1 Antibody, FITC conjugated
    Biotin CSB-PA883372LD01HU REV1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
  • 基因功能参考文献：
    1. These data indicate that dysregulation of cellular Rev1 levels leads to the accumulation of mutations and suppression of cell death, which accelerates the tumorigenic activities of DNA-damaging agents. PMID: 28498946
    2. The data directly show that, in the human genome, DNA Pol-eta and Rev1 bypass cyclobutane pyrimidine dimers and 6-4PP at replication forks, while only 6-4PP are also tolerated by a Rev3L-dependent gap-filling mechanism, independent of S phase. PMID: 27095204
    3. the catalytic function of REV1 is moderately or slightly altered by at least nine genetic variations, and the G4 DNA processing function of REV1 is slightly enhanced by the N373S variation, which might provide the possibility that certain germline missense REV1 variations affect the individual susceptibility to carcinogenesis by modifying the capability of REV1 for replicative bypass past DNA lesions and G4 motifs derived PMID: 26914252
    4. REV1 can promote PCNA monoubiquitylation after UV radiation through interacting with ubiquitylated RAD18. PMID: 26795561
    5. Saccharomyces cerevisiae PMID: 26903512
    6. Rev1 is indispensable for Translesion synthesis mediated by Poleta, Poliota, and Polkappa but is not required for TLS by Polzeta. PMID: 26680302
    7. Data suggest Rev1 protein recognition mechanism by Fanconi anemia-associated protein 20 (FAAP20). PMID: 26318859
    8. show that REV1 is a novel binding partner of the tumor suppressor p53 and regulates its activity PMID: 25614517
    9. Our results suggest for the first time that REV1 and REV3L SNPs might serve as potential predictive markers of outcome of cisplatin-based chemotherapy PMID: 24956248
    10. Structural studies suggest the possible involvement of XRCC1 and its associated repair factors, REV1 in post replication repair. PMID: 24409475
    11. The results show that human Rev1 disrupts G4 DNA structures and prevents refolding in vitro. PMID: 24366879
    12. the first structural insights into the regulation of human Rev1 for TLS polymerases. PMID: 23220741
    13. Rev1 but not Poleta depletion is epistatic to the lack of PCNA ubiquitination. PMID: 23761444
    14. a structural basis for understanding the recognition of the Rev1-CT by Y-family DNA polymerases PMID: 22691049
    15. Findings indicate that miR-96 regulates DNA repair and chemosensitivity by repressing RAD51 and REV1. PMID: 22761336
    16. Results suggest that abasic sites might be bypassed by single B- and Y-family pols or combinations, possibly by REV1 and pols iota, eta, and delta/PCNA at the insertion step opposite the lesion and by pols eta and delta/PCNA at the subsequent extension step. PMID: 20888339
    17. REV7 subunit of pol zeta mediated the interaction between REV3 and the REV1 C terminus. PMID: 22303021
    18. FAAP20 binding stabilizes Rev1 nuclear foci and promotes interaction of the Fanconi anemia core with PCNA-Rev1 DNA damage bypass complexes. PMID: 22266823
    19. Hsp90 promotes folding of REV1 into a stable and/or functional form(s) to bind to monoubiquitinated proliferating cell nuclear antigen in the regulation of translesion DNA synthesis-mediated mutagenesis PMID: 21690293
    20. WRN facilitates REV1-dependent translesion synthesis. PMID: 20691646
    21. the interaction between REV7 and REV3 creates a structural interface for REV1 binding PMID: 20164194
    22. REV1 and Polzeta facilitate repair of interstrand cross-links independently of PCNA monoubiquitination and Poleta, whereas RAD18 plus Poleta, REV1, and Polzeta are all necessary for replicative bypass of cisplatin intrastrand DNA cross-links. PMID: 20028736
    23. The results suggest that the positive charge on R357 could prevent interaction of REV1 with dGTP. PMID: 20059978
    24. UV-induced mutant frequencies at the HPRT locus were reduced up to 75% in cells with reduced levels of REV1 mRNA and data support that targeting the mutagenic translesion DNA replication pathway can greatly reduce the frequency of induced mutations. PMID: 12930947
    25. REV1 interacts with three Y-family DNA polymerases. PMID: 15189446
    26. REV1 interacts with pol eta in translesion synthesis of damaged DNA PMID: 15380106
    27. REV1-dependent processes are important determinants of cisplatin-induced genomic instability and the development of resistance. PMID: 16495473
    28. a novel biochemical activity of human REV1 protein, due to higher affinity for single-stranded DNA (ssDNA) than the primer terminus PMID: 16803901
    29. Rev1 is a polypeptide associated with Poleta. The study results suggest that arrested replication forks strengthen interactions among Poleta, Rad18/Rad6 and Rev1, consistent with the requirement for effective TLS by Poleta at sites of DNA lesions. PMID: 16824193
    30. Results support Phe257Ser and Ser257Ser genotypes are associated with a decreased risk for cervical carcinoma, while Asn373Ser and Ser373Ser genotypes increased the risk. PMID: 18470628
    31. Data show that PCNA ubiquitination and REV1 play distinct roles in the coordination of DNA damage bypass that are temporally separated relative to replication fork arrest. PMID: 18498753
    32. human REV1, apparently the slowest Y family polymerase, is kinetically highly tolerant to N(2)-adduct at G but not to O(6)-adducts. PMID: 18591245
    33. plays a role in mutagenesis and translesin DNA synthesis. (review) PMID: 18975621
    34. Poleta-REV1 interactions prevent spontaneous mutations, probably by promoting accurate translesion DNA synthesis past endogenous DNA lesions PMID: 19157994
    35. Novel structural features are important for providing Rev1 greater latitude in promoting efficient and error-free translesion DNA synthesis through the diverse array of bulky and potentially carcinogenic N(2)-deoxyguanosine DNA adducts in human cells. PMID: 19464298

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-Y family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 14060

    OMIM: 606134

    KEGG: hsa:51455

    STRING: 9606.ENSP00000258428

    UniGene: Hs.443077