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RCC1 Antibody

  • 货号：
    CSB-PA068328
  • 规格：
    ￥2024
  • 图片：
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RCC1 antibody.
    • Western blot analysis of extracts from LOVO cells, using RCC1 antibody.
    • Western blot analysis of extracts from HepG2 cells (Lane 2), cells (Lane 3) and cells (Lane 4), using RCC1 antiobdy. The lane on the left is treated with systhesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RCC1 Polyclonal antibody
  • Uniprot No.：
    P18754
  • 基因名：
    RCC1
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from Human RCC1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.
  • 基因功能参考文献：
    1. This study provides first evidence for a mutation in RCC1 as a breast cancer susceptibility allele PMID: 29363114
    2. Importin alpha3 facilitates the nuclear transport of the Ran guanine nucleotide exchange factor RCC1. PMID: 29042532
    3. this study has demonstrated that EBNA1 and RCC1 closely associate on the chromosomes during metaphase, suggesting an essential role for the interaction during this phase, perhaps in tethering EBNA1 to mitotic chromosomes. PMID: 28284242
    4. RCC1 expression enables the proliferation of cells that sustain DNA damage. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. PMID: 26864624
    5. RCC1 expression is frequently lost in poorly differentiated gastric cell lines and gastric carcinoma tissues. Loss of RCC1 expression is correlated with tumor differentiation and depth of invasion. PMID: 26823742
    6. stress can disrupt the Ran gradients through RCC1-dependent and RCC1-independent mechanisms PMID: 25452301
    7. PI3Kbeta regulates the nuclear envelope through upstream regulation of RCC1 and Ran PMID: 25348717
    8. RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase. PMID: 23536659
    9. RCC1 can induce bipolar spindle formation. Remarkably, RCC1 beads oscillate within spindles from pole to pole, a behavior that could be converted to a more typical, stable association by the addition of a kinesin together with RCC1. PMID: 22215983
    10. discovery of the first alpha-N-methyltransferase, which we named N-terminal RCC1 methyltransferase (NRMT). PMID: 20668449
    11. The s find that RCC1 uses a conformationally flexible loop region we have termed the switchback loop in addition to its N-terminal tail to bind to the nucleosome. PMID: 20347844
    12. RanBP3 associates with the Ran-specific guanine nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1). PMID: 11932251
    13. These results indicate that the generation of Ran-GTP in the vicinity of chromosomes by RCC1 is important for the fidelity of mitotic spindle assembly in human cells. PMID: 12194828
    14. RCC1 release from chromatin in vitro requires a factor(s) distinct from, or in addition to, Ran PMID: 14565978
    15. phosphorylation of the NLS of RCC1 is required to prevent the binding of importin alpha and beta to RCC1, thereby allowing RCC1 to couple RanGTP production to chromosome binding PMID: 15014043
    16. This 30 kb deletion contains the exons coding for the RCC1-like domain of RPGR. It is the first report of a macrodeletion that spans the entire RCC1-like domain of RPGR in X-linked retinitis pigmentosa patients PMID: 16052169
    17. HSV-1 replication is blocked in cell line tsBN2 encoding a temperature sensitive RCC1 protein. PMID: 17251553
    18. Propose bimodal attachment mechanism for RCC1 in which the tail promotes stable RCC1 association with chromatin through DNA binding in an alpha-N-methylation-dependent manner. PMID: 17435751
    19. These results show that human RCC1 isoforms have distinct chromatin binding properties, different molecular interactions, and are selectively regulated by phosphorylation, as determined by their different NTRs. PMID: 17855385
    20. chromosome 1p36 was established to have linkage for adhd. PMID: 18439570
    21. A review of the function and structure of RCC1 and RCC1 superfamily genes and proteins. PMID: 18442486
    22. reduced binding affinity of RCC1 to the mitotic chromosome, which led to a reduction of RanGTP level. PMID: 18712773
    23. The N-terminal tail of RCC1 is essential for association with DNA but inhibits histone binding. PMID: 18762580
    24. Therefore, we propose that RCC1 reads the histone code created by caspase-activated Mst1 to initiate apoptosis by reducing the level of RanGTP in the nucleus. PMID: 19060893

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm.
  • 数据库链接：

    HGNC: 1913

    OMIM: 179710

    KEGG: hsa:1104

    UniGene: Hs.469723