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RBFOX2 Antibody

  • 货号：
    CSB-PA019439LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: NIH/3T3 whole cell lysate
      All lanes: RBFOX2 antibody at 6μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42, 41, 38, 40, 48, 44 kDa
      Observed band size: 42 kDa
    • IHC image of CSB-PA019439LA01HU diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA019439LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA019439LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RBFOX2 Polyclonal antibody
  • Uniprot No.：
    O43251
  • 基因名：
    RBFOX2
  • 别名：
    ibody; Fox 1 homologue antibody; Fox-1 homolog B antibody; FOX2 antibody; FXH antibody; Hexaribonucleotide-binding protein 2 antibody; HNRBP2 antibody; RBFOX2 antibody; Repressor of tamoxifen transcriptional activity antibody; RFOX2_HUMAN antibody; RNA binding motif protein 9 antibody; RNA binding protein 9 antibody; RNA binding protein fox-1 homolog 2 antibody; RNA-binding motif protein 9 antibody; RNA-binding protein 9 antibody; RTA antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human RNA binding protein fox-1 homolog 2 protein (8-110AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RBFOX2 Antibody (CSB-PA019439LA01HU)，的标记方式是Non-conjugated。对于RBFOX2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA019439LB01HU RBFOX2 Antibody, HRP conjugated ELISA
    FITC CSB-PA019439LC01HU RBFOX2 Antibody, FITC conjugated
    Biotin CSB-PA019439LD01HU RBFOX2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
  • 基因功能参考文献：
    1. Rbfox2 nonsense mutation is associated with hypoplastic left heart syndrome. PMID: 27485310
    2. Data show that while RBFOX1 and RBFOX2 do not mediate neuron-specific processing of UBE3A-ATS, these proteins play important roles in developing neurons and are not completely functionally redundant. PMID: 27146458
    3. Results showed that the expression patterns of these genes were indicative of the onset of EMT in in-vitro models, but not in tissue samples. However, the ratio between ESRP1 or ESRP2 and RBFOX2 significantly decreased during EMT and positively correlated with the EMT-specific phenotype in cell models. Low ESRP1/RBFOX2 ratio value was associated with a higher risk of metastasis in early breast cancer patients. PMID: 27911856
    4. Rbfox2 modulates the functions of vascular CaV1.2 calcium channel by dynamically regulating the expressions of alternative exons 9* and 33, which in turn affects the vascular myogenic tone. PMID: 28993448
    5. RBFOX2 dysregulation by dominant-negative RBFOX2 is an early pathogenic event in diabetic hearts. PMID: 27239029
    6. RBFox2 interactis with chromatin in a nascent RNA-dependent manner. RBFox2 inactivation eradicates PRC2 targeting on the majority of bivalent gene promoters and leads to transcriptional de-repression. PMID: 27211866
    7. Some of the widespread cellular functions of Rbfox2 protein are attributable to regulation of miRNA biogenesis, and might include the mis-regulation of miR-20b and miR-107 in cancer and neurodegeneration. PMID: 27001519
    8. RBFOX proteins can facilitate the splicing of micro-exons. We also found that PTBP1 likely regulates the inclusion of micro-exons, possibly by repressing the inclusion of micro-exons that are enhanced by RBFOX proteins and other splicing factors.[RBFOX] PMID: 25524026
    9. CPSF2 and SYMPK, are RBFOX2 cofactors for both inclusion and exclusion of internal exons. PMID: 25921069
    10. RBFOX2 SNPs showed evidence for effects across multiple reading and language traits. PMID: 25065397
    11. Results show that the conserved Rbfox2 RNA binding protein regulates 30% of the splicing transitions observed during myogenesis and is required for the specific step of myoblast fusion. PMID: 25087874
    12. MBNL1 and RBFOX2 cooperate to establish a splicing programme involved in pluripotent stem cell differentiation. PMID: 24048253
    13. RBFOX2 polymorphism is associated with breast cancer. PMID: 23143756
    14. RBFOX2 drives mesenchymal tissue-specific splicing in both normal and cancer tissues. PMID: 23149937
    15. FOX-2 is involved in splicing of ataxin-2 transcripts and that this splicing event is altered by overexpression of ataxin-1 PMID: 22666429
    16. functional significance of EMT-associated alternative splicing depletion of RBFOX2 in mesenchymal cells PMID: 21876675
    17. This study characterizes the mechanism by which RBFOX2 regulates protein 4.1R exon 16 splicing through the downstream intronic element UGCAUG. PMID: 22083953
    18. the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism PMID: 21747913
    19. Fox-2 plays an integral role in the regulation of LH2 splicing and knockdown of Fox-2 may suggest a novel approach to strategies directed against scleroderma. PMID: 20131247
    20. Fox-1 and Fox-2 isoforms specifically activate splicing of neuronally regulated exons, which requires UGCAUG enhancer elements PMID: 16260614
    21. Fox-1 and Fox-2 splicing factors have roles in alternative splicing of protein 4.1R PMID: 16537540
    22. Fox-1/Fox-2 proteins block prespliceosome complex formation at two distinct steps through binding to two functionally important UGCAUG elements. PMID: 18573872
    23. These results establish hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance their ability to antagonize ASF/SF2. PMID: 18573884
    24. predict thousands of Fox-1/2 targets with conserved binding sites, at a false discovery rate of approximately 24%, including many validated experimentally, suggesting a surprisingly extensive splicing regulatory networks PMID: 18794351
    25. These findings suggest that FOX2 functions as a critical regulator of a splicing network, and that FOX2 is important for the survival of human embryonic stem cells. PMID: 19136955

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    HGNC: 9906

    OMIM: 612149

    KEGG: hsa:23543

    STRING: 9606.ENSP00000413035

    UniGene: Hs.282998