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  4. RAD54L Antibody

RAD54L Antibody

  • 货号：
    CSB-PA070022
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q92698
  • 基因名：
    RAD54L
  • 别名：
    DNA repair and recombination protein RAD54 like antibody; DNA repair and recombination protein RAD54-like antibody; hHR 54 antibody; hHR54 antibody; HR 54 antibody; HR54 antibody; hRAD 54 antibody; hRAD54 antibody; RAD 54A antibody; RAD 54L antibody; RAD54 (S. cerevisiae) like antibody; RAD54 homolog antibody; RAD54 like (S. cerevisiae) antibody; RAD54 like antibody; RAD54 like protein antibody; RAD54_HUMAN antibody; RAD54A antibody; RAD54L antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human Rad54.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA.
  • 基因功能参考文献：
    1. Data show that the RAD54 N-terminal domain (NTD) is responsible for initiation of branch migration (BM) through two coupled, but distinct steps; specific binding to Holliday junctions and RAD54 oligomerization. PMID: 29295984
    2. Nap1 binds to RAD54. PMID: 24798879
    3. TAF12 and NFYC are transcription factors that regulate the epigenome, whereas RAD54L plays a central role in DNA repair PMID: 25965574
    4. support a model in which RAD54L and RAD54B counteract genome-destabilizing effects of direct binding of RAD51 to dsDNA in tumor cells PMID: 25765654
    5. The RAD54L polymorphism (2290C/T) can be used as a genetic marker inside the consensus deletion region at 1p32 in human meningiomas. PMID: 12614485
    6. Shortened telomeres in murine scid cells expressing mutant RAD54L coincide wirth reduction in recombination at telomeres. PMID: 15975611
    7. hRad54, a Swi2/Snf2 protein, binds HJ-like structures with high specificity and promotes their bidirectional branch migration in an ATPase-dependent manner PMID: 16862129
    8. Some immortal cells use the alternative lengthening of telomeres (ALT) pathway to maintain their telomeres instead of telomerase. This is the first genetic evidence that Rad54 is dispensable for the ALT pathway. PMID: 17054727
    9. RAD54 is recruited by RAD51-ssDNA filament to the chromatin of the intact chromosome and it remodels that chromatin to facilitate accessibility for strand exchange PMID: 17417655
    10. analysis of human rad54 protein interactions with branched DNA molecules PMID: 17545145
    11. Rad54 protein causes dissociation of joint molecules by ATP-dependent branch-migration and therefore plays an important role in double strand DNA break repair. PMID: 17660833
    12. germline mutations in RAD51, RAD51AP1, RAD51L1, RAD51L3, RAD52 and RAD54L are unlikely to be causal of an inherited predisposition to CLL. PMID: 18203022
    13. Rad51 protein stimulates the branch migration activity of Rad54 protein.( PMID: 18617519

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 数据库链接：

    HGNC: 9826

    OMIM: 603615

    KEGG: hsa:8438

    STRING: 9606.ENSP00000361043

    UniGene: Hs.642042