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  4. RAD23A Antibody

RAD23A Antibody

  • 中文名称：
    RAD23A兔多克隆抗体
  • 货号：
    CSB-PA040140
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P54725
  • 基因名：
  • 别名：
    hHR 23A antibody; hHR23A antibody; HR23A antibody; MGC111083 antibody; RAD 23a antibody; RAD23 homolog A (S. cerevisiae) antibody; RAD23 homolog A antibody; RAD23 yeast homolog A antibody; RAD23A antibody; RD23A_HUMAN antibody; UV excision repair protein RAD23 antibody; UV excision repair protein RAD23 homolog A antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human Rad23A.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:200-1:1000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.; Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.; (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
  • 基因功能参考文献：
    1. Data indicate that HR23A protein-depleted cells exhibit enhanced autophagy when treated with DNA-damaging agents. PMID: 27613096
    2. HR23A role in DNA reapair, in protein degradation and stability, tumorigenesis and neurodegenerative disorders [review] PMID: 27771451
    3. hHR23A associates with Chk1 through its ubiquitin-associated domains, and knockdown of hHR23A increases and stabilizes the protein level of Chk1 and its phosphorylation at S347. PMID: 26296656
    4. Data indicate that phosphorylation provides a mechanism to regulate Rad23/proteasome interaction. PMID: 25311859
    5. Here, we show that hHR23A utilizes both the UBA2 and XPCB domains to form a stable complex with Vpr, linking Vpr directly to cellular DNA repair pathways and their probable exploitation by the virus. PMID: 24318982
    6. this study identified RAD23A as a novel negative regulator of RIG-I/MDA5 mediated anti-virus response. PMID: 23357418
    7. Determined is the three-dimensional structure of its ubiquitin-like (UbL) domain by X-ray crystallography. PMID: 21047872
    8. Vpr promotes hHR23A-mediated protein-ubiquitination, and down-regulation of hHR23A using RNAi significantly reduced viral replication in non-proliferating MAGI-CCR5 cells and primary macrophages PMID: 20614012
    9. involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination PMID: 11788722
    10. structures of the UBA domains of HHR23A reveal a conserved hydrophobic surface for protein-protein interactions PMID: 12079361
    11. the solution structures of the HHR23A Ubl domain PMID: 12970176
    12. Adopts a closed conformation and binds to the proteasomal subunit S5a thereby changing its own conformation. PMID: 14557549
    13. hHR23 binds to polyubiquitylated p53 via its carboxyl-terminal ubiquitin-associated (Uba) domain shielding p53 from deubiquitylation PMID: 14645509
    14. Data suggest that the UBL domain of HHR23A negatively regulates polyubiquitin/UBA interactions and identify leucine 8 of ubiquitin as an important determinant of chain recognition. PMID: 15321727
    15. hHR23A regulates the function of xeroderma pigmentosum C by its association with the nucleotide excision repair activator p53 PMID: 16105547
    16. Ufd4, the E3 component of the UFD pathway, is involved in controlling the degradation of Rad4, and Ufd4 and Rad23 exhibit a synthetic inhibitory effect on Rad4 degradation PMID: 16430867
    17. hHR23B thus plays a critical role in the activation and function of p53 after specific genotoxic exposures. PMID: 16924240
    18. hHR23a and hPLIC2 interact via UBL/UBA domain interactions PMID: 17098253
    19. Pyramidal crystals of the UbL domain of hHR23A were diffracted to beyond 2 A resolution and the structure was solved by molecular replacement. PMID: 19724136

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD23 family
  • 数据库链接：

    HGNC: 9812

    OMIM: 600061

    KEGG: hsa:5886

    STRING: 9606.ENSP00000467024

    UniGene: Hs.643267