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  4. RAD1 Antibody

RAD1 Antibody

  • 货号：
    CSB-PA019252GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    O60671
  • 基因名：
  • 别名：
    Cell cycle checkpoint protein Hrad1 antibody; Cell cycle checkpoint protein Rad 1 A / B antibody; Cell cycle checkpoint protein RAD1 antibody; Checkpoint control protein HRAD1 antibody; Checkpoint control protein RAD1 antibody; DNA repair exonuclease antibody; DNA repair exonuclease rad1 antibody; DNA repair exonuclease rad1 homolog antibody; DNA repair exonuclease REC1 antibody; DNA repair protein RAD1 antibody; EC 3.1.11.2 antibody; Exonuclease homolog RAD1 antibody; GTP-binding protein RAD antibody; hRAD 1 antibody; hRAD1 antibody; MGC77779 antibody; RAD 1 antibody; RAD antibody; RAD1 antibody; RAD1 homolog (S. pombe) antibody; RAD1 homolog antibody; Rad1 like DNA damage checkpoint antibody; Rad1 like DNA damage checkpoint protein antibody; RAD1; S. pombe; homolog of antibody; Rad1-like DNA damage checkpoint protein antibody; RAD1_HUMAN antibody; Ras associated with diabetes antibody; REC 1 antibody; REC1 antibody; RRAD antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human RAD1
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • 基因功能参考文献：
    1. Dominant alleles of RAD51, TP53 and XRCC1 combined genotypes indicated a strong protective role against hereditary breast cancer. PMID: 26954070
    2. Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding. PMID: 26088138
    3. Data show models for the ternary PCNA/FEN1/DNA and Rad9-Rad1-Hus1 (9-1-1 complex)/FEN1/DNA assemblies. PMID: 22586102
    4. The RAD1 is loaded to damaged sites where it serves as a platform for the selective recruitment of checkpoint and repair proteins. PMID: 21978893
    5. CK2 plays a crucial role in the ATR-dependent checkpoint pathway through its ability to phosphorylate Ser-341 and Ser-387 of the Rad9 subunit of the Rad9-Hus1-Rad1 complex PMID: 20545769
    6. 9-1-1 complex is a component of the mismatch repair involved in MNNG-induced damage response. PMID: 20188637
    7. Rad9-Rad1-Hus1 complex enhances in vitro activity of 8-oxoguanine DNA glycosylase. PMID: 19615952
    8. Rad9, Hus1, and Rad1 heterotrimeric complex chromatin binding is a proximal event in the checkpoint signaling cascade PMID: 12228248
    9. RAD1 is a potential intrinsic chaperone in the stabilization of HUS1 for the heterotrimeric (RAD9-RAD1-HUS1) checkpoint complex formation. PMID: 15122316
    10. The human Rad9/Rad1/Hus1 complex interacts with and stimulates DNA polymerase beta activity. PMID: 15314187
    11. complex with rad9 and hus1 is a damage-specific activator of flap endonuclease 1 PMID: 15556996
    12. The long-patch base excision machinery is an important target of the Rad9-Rad1-Hus1 complex, thus enhancing the quality control of DNA. PMID: 15871698
    13. PCNA and the Rad9/Rad1/Hus1 complex can independently bind and activate Fen1; acetylation of Fen1 by p300-HAT abolished the stimulatory effect of the complex but not that of PCNA, suggesting a possible mechanism of regulation of this repair pathway PMID: 16216273
    14. human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
    15. These data provide in vivo evidence that the human 9-1-1 complex participates in DNA repair in addition to its previously described role in DNA damage sensing. PMID: 16814252
    16. Human NEIL1 DNA glycosylase activity is significantly stimulated by hRad1 and by the Rad9/Rad1/Hus1 heterotrimer. PMID: 17395641
    17. we report successful tri-cistronic cloning, overexpression and purification of a three-protein complex of Rad9, Rad1 and Hus1 using a single hexa-histidine tag. PMID: 17493829
    18. Jab1 physically associates with the 9-1-1 complex; this association is mediated through direct interaction between Jab1 and Rad1, one of the subunits of the 9-1-1 complex PMID: 17583730
    19. Human thymine DNA glycosylase activity is significantly stimulated by hHus1, hRad1, hRad9 separately, and by the 9-1-1 complex. PMID: 17855402
    20. The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
    21. The interdomain connecting loops (IDC loop) of hRad9, hHus1, and hRad1 are largely divergent and unique structural features of the 9-1-1 complex that are proposed to contribute to DNA damage recognition. PMID: 19535328

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Rad1 family
  • 组织特异性：
    Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • 数据库链接：

    HGNC: 9806

    OMIM: 603153

    KEGG: hsa:5810

    STRING: 9606.ENSP00000340879

    UniGene: Hs.38114