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  4. Phospho-WAS (Y290) Antibody

Phospho-WAS (Y290) Antibody

  • 货号：
    CSB-PA060081
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P42768
  • 基因名：
  • 别名：
    Eczema thrombocytopenia antibody; IMD2 antibody; SCNX antibody; THC antibody; THC1 antibody; Thrombocytopenia 1 (X linked) antibody; U42471 antibody; Was antibody; WASp antibody; WASP_HUMAN antibody; Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody; Wiskott Aldrich syndrome antibody; Wiskott Aldrich syndrome protein antibody; Wiskott-Aldrich syndrome protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Human WASP around the phosphorylation site of Y290.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
  • 基因功能参考文献：
    1. study indicates that anti-inflammatory macrophage function and mucosal immune tolerance require both WASP and DOCK8, and that IL-10 signalling modulates a WASP-DOCK8 complex PMID: 29725003
    2. A novel WASP mutation (I290T) was found in an X-linked neutropenia patient and in his heterozygous mother. This mutation was located in the GTPase-binding domain. PMID: 28956125
    3. In a model of sterile inflammation utilizing TLR4 ligation followed by ATP or nigericin treatment, inflammasome activation is enhanced in monocytes from WAS patients. PMID: 29146903
    4. Despite the lack of typical clinical manifestations of WAS, low expression of WASP could be associated with the pathogenesis of a subtype of inflammatory bowel disease patients. PMID: 29358862
    5. expression of WASP inversely correlates with BCR-ABL1 levels and the progression of the disease in Chronic myeloid leukemia patients. downregulation of WASP contributes to the resistance to apoptosis and to BCR-ABL1-induced tumorigenesis. PMID: 29022901
    6. The coverage and depth of WASP were extremely low. PMID: 28901403
    7. WIP residues 454-456 are the major contributor to WASp affinity, and residues 449-451 were found to have the largest effect upon WASp ubiquitylation and, presumably, degradation. PMID: 29215267
    8. Novel WASP mutations were found in two patients with X-linked thrombocytopenia and their families. PMID: 28641574
    9. High WASP expression is associated with lung cancer invasion. PMID: 28351346
    10. A Treg-specific role for WASP is required for prevention of Th2 effector cell differentiation and allergic sensitization to dietary antigens. PMID: 27643438
    11. WASP and SCAR drive pseudopod formation and are conserved in actin-filled pseudopod-based motility. PMID: 28473602
    12. s show that knock-down of WASp or expression of Y102F mutant of WASp decreases colony formation and in vivo tumor growth. Results show that WASp is a novel substrate of ALK and has a critical role in regulating invasiveness and oncogenesis of ALCL. PMID: 27694894
    13. this study describes an Iranian boy with Wiskott-Aldrich syndrome with a novel WASP mutation PMID: 26993433
    14. The inducible recruitment of WASp to the TCR-CD3 complex is partially dependent of tyrosine phosphorylation of Cd3e. PMID: 26342115
    15. retrospectively investigated the outcome of hematopoietic stem cell transplantation in a cohort of 24 patients with the X-linked thrombocytopenia phenotype and mutations in the WAS gene PMID: 25388447
    16. This suggests that N-WASP's failure to compensate for WASP in rescuing chemotaxis could be due to the absence of this I30 region. PMID: 26463123
    17. N-WASP is downregulated in clear cell renal cell carcinoma PMID: 25115631
    18. Studies indicate that mutations in the Wiskott-Aldrich syndrome protein (WASp) gene cause a continuum of clinical symptoms ranging from intermittent X-linked thrombocytopenia to full classical Wiskott-Aldrich syndrome (WAS). PMID: 26159751
    19. Platelet actin nodule formation is dependent on WASp and the ARP2/3 complex. PMID: 26028144
    20. conclude that tyrosine phosphorylation of WIP is a crucial regulator of WASP stability and function as an actin-nucleation-promoting factor PMID: 25413351
    21. WASP, RUNX1, and ANKRD26 genes are important for normal TPO signaling and the network underlying thrombopoiesis. PMID: 26175287
    22. The introduction of functional WASp by GT corrected the alterations of both central and peripheral B cell tolerance checkpoints. WASp plays an important role in the establishment and maintenance of B cell tolerance in humans. PMID: 26368308
    23. We describe two Malay patients with classical Wiskott-Aldrich Syndrome with two different mutations in the WASP gene PMID: 26277674
    24. we identify small ubiquitin-related modifier (SUMO)ylation as a novel posttranslational modification of WASp PMID: 26261240
    25. A total of 60 unique WAS mutations were identified in Chinese patients, including 20 novel mutations and 8 hotspots, from 75 unrelated families with a total of 81 affected members. PMID: 25931402
    26. studies discovered that HMGB1 suppressed phosphorylation, nuclear translocation, and activation of CREB, by inhibiting nuclear translocation of PKA catalytic subunit PMID: 25277185
    27. data suggest that missense mutations WASPRL46P or WASPRA47D affect the activity of WASP in T cell chemotaxis probably by affecting the turnover of the protein. PMID: 25200405
    28. An indispensable relationship between nuclear-WASp- and hSWI/SNF-complexes in gene activation and molecular distinctions in TH cells that might contribute to disease severity in the X-linked thrombocytopenia/Wiskott-Aldrich syndrome clinical spectrum. PMID: 25253772
    29. Data indicate the WASp-interacting protein (WIP)-Wiskott-Aldrich syndrome protein (WASp) interaction in the regulation of actin-dependent processes. PMID: 24962707
    30. WASp and WAVE2 differ in their dynamics and their associated proteins PMID: 25342748
    31. WAS gene mutation is associated with X-linked thrombocytopenia in three males with normal sized platelets. PMID: 25154619
    32. WASP deficiency perturbs the homeostasis of B-cell compartment in humans. PMID: 24369837
    33. Data indicate that mycolactone analogues bind Wiskott-Aldrich syndrome proteins (WASP} with IC50 in the 50-10 muM range. PMID: 25158122
    34. These findings support a contributory role for defective Breg cells in the development of WAS-related autoimmunity PMID: 24945741
    35. study unveils an ARP2/3:VCA-independent function of nuclear-WASp in TH1 gene activation that is uncoupled from its cytoplasmic role in actin polymerization. PMID: 24872192
    36. Pro373Ser mutation reduces Tyr291 phosphorylation and prevents conformational changes required for WASP activity in chemotaxis and T-cell activation. PMID: 24440360
    37. Missense mutation in the WAS gene is associated with intermittent X-linked thrombocytopenia. PMID: 24115682
    38. We conclude that WASp function restricts TGF-b1 secretion in a Cdc42- and Src family kinase-dependent manner and independently of actin assembly PMID: 24133214
    39. We present two cases of WAS in neonates with WAS gene mutations PMID: 23301916
    40. Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity. PMID: 23455509
    41. Data indicate that slit2N alters the localization and binding of Robo1 to WASp and LSP1 in HIV-1-gp120-treated immature dendritic cells (iDCs). PMID: 23119100
    42. Report five previously reported mutations and six novel mutations in WASP gene in Iranian Wiskott-Aldrich patients. PMID: 23264413
    43. this is the first report describing TTP in WAS patients with novel mutation in the WASP gene. PMID: 23237501
    44. Despite mediating enhanced actin polymerization, EVH1 missense-mutated human proteins did not function fully in mouse cells, even when overexpressed. Mutant protein retention in podosomes was impaired & associated with low WASp Tyr phosphorylation. PMID: 23160469
    45. study describes that both N-WASp and WASp participate in the inhibition of NK-cell chemotaxis in response to NKG2D WASp engagement, and that this effect is not dependent on the regulation of F-actin dynamics PMID: 22585739
    46. WASP-deficient T cells migrated in a normal proportion towards CXCL12, CCL19 and CCL21, but displayed an increased adhesion and elongation on ICAM-1 PMID: 22804504
    47. data suggest that regulated degradation of activated WASp might be an efficient strategy by which the duration and localization of actin rearrangement and the intensity of T-cell activation are controlled. PMID: 22665495
    48. The wild-type WASp, but not the mutant restored adhesion capacity, spreading morphology, and cytoskeletal reorganization. PMID: 22311461
    49. Mutation in WASP gene is associated with Wiskott-Aldrich syndrome and X-linked thrombocytopenia. PMID: 22038941
    50. the c.273+11dup change within the WAS gene was observed in patients showing symptoms consistent with the Wiskott-Aldrich syndrome; concluded that the presence of the additional C in the WAS gene is a functionally neutral polymorphism PMID: 21711396

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  • 相关疾病：
    Wiskott-Aldrich syndrome (WAS); Thrombocytopenia 1 (THC1); Neutropenia, severe congenital, X-linked (XLN)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Nucleus.
  • 组织特异性：
    Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
  • 数据库链接：

    HGNC: 12731

    OMIM: 300299

    KEGG: hsa:7454

    STRING: 9606.ENSP00000365891

    UniGene: Hs.2157