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  4. Phospho-PRKCB (Thr641) Antibody

Phospho-PRKCB (Thr641) Antibody

  • 货号：
    CSB-PA206081
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCβ (phospho-Thr641) antibody.
    • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody.
    • Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PRKCB Polyclonal antibody
  • Uniprot No.：
    P05771
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from Human PKCb
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
  • 基因功能参考文献：
    1. Tumor suppressor activity of protein kinase C-beta.[review] PMID: 28571764
    2. The results demonstrate a direct relationship between SP1 binding and protein kinase CbetaII (PKCbetaII) transcription, and further suggest that this transcription factor is a contributor to the pathobiology of chronic lymphocytic leukaemia and potentially other malignant cells where PKCbetaII is overexpressed. PMID: 28233872
    3. An exaggerated vasoconstriction response to dexmedetomidine, an alpha-2 adrenergic agonist, has been associated with SNP rs9922316 in the gene for protein kinase C type beta ( PRKCB). PMID: 28482761
    4. PKC beta would sensitize cervical cancer cells to chemotherapy via reducing the chemotherapy induced autophagy in cancer cells. PMID: 28246354
    5. Loss of PRKCB2 expression is associated with colorectal cancer. PMID: 26989024
    6. significantly different gene expressions of BECN1 and PRKCB between the control and the Alzheimer's disease (AD) groups and of CDKN2A between the control and the preclinical AD groups, are reported. PMID: 26510741
    7. a primary functional variant of PRKCB (rs35015313) was identified by genotype imputation using a phased panel of 1,070 Japanese individuals from a prospective, general population cohort study and subsequent in vitro functional analyses. These results may lead to improved understanding of the disease pathways involved in primary biliary cholangitis. PMID: 28062665
    8. our findings identify PRKCB gene as a novel candidate gene for familial Meniere's Disease (MD )and its expression gradient in supporting cells of the organ of Corti deserves attention, given the role of supporting cells in K(+ )recycling within the endolymph, and its apical turn location may explain the onset of hearing loss at low frequencies in MD PMID: 27329761
    9. Activation of the Pro-Oxidant PKCbetaII-p66Shc Signaling Pathway Contributes to Pericyte Dysfunction in Skeletal Muscles of Patients With Diabetes With Critical Limb Ischemia PMID: 27600065
    10. Taken together, these data argue for a complex mechanism of PKC-beta-dependent regulation of SHCA (p66) activation involving Ser(139) and a motif surrounding Ser(213). PMID: 27624939
    11. The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
    12. Bone marrow stroma-induced resistance of chronic lymphocytic leukemia cells to arsenic trioxide involves Mcl-1 upregulation and is overcome by inhibiting the PI3Kdelta or PKCbeta signaling pathways. PMID: 26540567
    13. PPAR-delta and NKIRAS1 are downstream mediators in the PRKCB pathway in human umbilical vein endothelial cells. PMID: 26459836
    14. Lower hydrogen sulfide is associated with cardiovascular mortality, which involves PKCBII/Akt pathway in chronic hemodialysis patients. PMID: 26439941
    15. Study found a significant decrease of PRCKB1 mRNA expression in subsyndromal symptomatic depression, suggesting PRKCB1 might be a candidate gene and biomarker PMID: 26343587
    16. PKCbetaII inhibits the ubiquitination of beta-arrestin2 in an autophosphorylation-dependent manner PMID: 26545496
    17. Ionizing radiation-induced eNOS activation in human vascular endothelial cells is attributed to both the up-regulation of PKC-betaII and the increase in ROS generation which were independent of each other. PMID: 25869503
    18. Effect of PKC-beta Signaling Pathway on Expression of MCP-1 and VCAM-1 in Different Cell Models in Response to Advanced Glycation End Products PMID: 26008233
    19. Direct interaction between the two proteins leads to Apoptin-induced activation of PKC and consequently activated PKCbetaI mediates phosphorylation of Apoptin to promote its tumour-specific nuclear translocation and cytotoxic function. PMID: 25828882
    20. Gene fusions involving PRKC genes occur in several morphological and clinical subsets of benign fibrous histiocytoma, but they seem to account for only a minority of the cases. PMID: 26121314
    21. PMA primed PBTLs for polarization under flow, with protein kinase C (PKC)-delta enriched in the leading edge, PKC-betaI in the microtubule organizing center, and PKC-betaII in the uropod and peripheral region. PMID: 25548371
    22. Our data indicate a new direction for LOX-1 regulation by the modulation of the PKCbeta/NAPDH oxidase/SIRT1/HSF1 mechanism PMID: 25982096
    23. Hyperoxia can increase the expression of PKCbeta in alveolar epithelial cells and production of mitochondrial reactive oxygen species and decrease mitochondrial membrane potential. PMID: 25815500
    24. human AKAP79-anchored PKC selectively phosphorylates the Robo3.1 receptor subtype on serine 1330 PMID: 25882844
    25. that the high-concentration glucose-induced disruption of endothelial adherens junctions is mediated by tyrosine phosphorylation of vascular endothelial cadherin through PKC-beta and myosin light chain phosphorylation PMID: 25927959
    26. PKCbeta2 inhibition protects mice from gut ischemia-reperfusion injury by suppressing the adaptor p66(Shc)-mediated oxidative stress and subsequent apoptosis. PMID: 24722289
    27. The detected PDPN-PRKCB, CD63-PRKCD and LAMTOR1-PRKCD gene fusions are all predicted to result in chimeric proteins consisting of the membrane-binding part of PDPN, CD63 or LAMTOR1 PMID: 24721208
    28. PRKCB2 is specifically required for mTORC2-dependent AC9 activation and back retraction during neutrophil chemotaxis. PMID: 24600048
    29. Pharmacological inhibition PRKCB1 via their specific inhibitors and neutralisation of O2 (*-) by a cell-permeable superoxide dismutase mimetic. PMID: 24936444
    30. isoform betaII plays a central role in the PKC-dependent regulation of Kv1.5/Kvbeta1.2 channels. PMID: 24682423
    31. shRNA knockdown of PKCbeta reduced cellular proliferation, colony formation, and migratory capacity of melanoma cells and also reduced lung colonization of stably transduced melanoma cells in mice. PMID: 24406113
    32. STAT3 bound to previously undescribed negative regulatory elements within the promoter of PRKCB, which encodes PKCbetaII. PMID: 24550541
    33. Protein kinase C beta is important for the regulation of NHE-1 activity, which is associated with ERK1/2-p90RSK signaling pathways as a kinase of NHE-1 in cortical neuronal cells exposed to glutamate. PMID: 24378530
    34. Vimentin is a phosphorylation target of PKC-beta in MCP-1-treated monocytes and that PKC-beta phosphorylation is essential for vimentin secretion. PMID: 23974215
    35. CD40L may contribute to atherogenesis via PRKCB by activating endothelial cells and recruiting monocytes to them. PMID: 24039784
    36. established an important role for PKCbeta1 in PANC1 cells suggesting it would act as a suppressor of tumorigenic behavior in pancreatic cancer PMID: 23695799
    37. Data suggest that hyperglycemia promotes cerebral-barrier dysfunction through activation of PKCbeta and consequent stimulation of oxidative stress and tight junction dissolution. PMID: 23617822
    38. This review summarizes the current knowledge of both PKCbeta and PKCdelta isoforms during atherogenesis, and addresses differential roles and disputable observations of PKC isoforms. PMID: 24440741
    39. The translocation of PKCBetaII was induced by soluble amyloid Beta precursor protein alpha. PMID: 23905995
    40. Normalization of glucose levels and silencing PKCB activity neutralized the effects of hyperglycemia on occludin and RhoA/Rho-kinase/MLC2 expression, localization, and activity and consequently improved in vitro blood-brain barrier integrity and function. PMID: 23963366
    41. HIF1alpha and PKCbeta have roles in mediating the effect of oxygen and glucose during wound healing PMID: 23562913
    42. PKCbeta is the isoform responsible for Syk negative regulation PMID: 23960082
    43. PKCalpha and PKCbeta cooperate in IL-2 transcriptional transactivation in primary mouse T cells independently of the actions of PKCtheta;. PMID: 23439007
    44. Protein kinase Cbeta critically regulates dopamine D2 autoreceptor-activated dopamine transporter trafficking. PMID: 23458603
    45. Genetic variation in protein kinase C type beta may contribute toward the interindividual variation in DHV constriction responses to alpha2-AR activation by the agonist dexmedetomidine PMID: 23337848
    46. PKCbeta plays an important role in Acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin lymphoma survival. PMID: 21997316
    47. Inhibition of PKCbeta enhanced apoptosis of human autoreactive B cells. PMID: 23280626
    48. The expression of protein kinase C (PKC)-betaII and the subsequent activation of NF-kappaB in bone marrow stromal cells are prerequisites to support the survival of malignant B cells. PMID: 23328482
    49. Comp of gene expression between PROX1-overexpressing and mock-transfected cells revealed that the expression of PRKCB2 is down-regulated in PROX1-overexpressing cells. A PRKCB inhibitor suppressed growth of control cells more than PROX1-expressing cells. PMID: 22833470
    50. Estrogen receptor-alpha, RBCK1, and protein kinase C beta 1 cooperate to regulate estrogen receptor-alpha gene expression. PMID: 23042805

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 数据库链接：

    HGNC: 9395

    OMIM: 176970

    KEGG: hsa:5579

    STRING: 9606.ENSP00000305355

    UniGene: Hs.460355