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  4. Phospho-PLCB3 (S537) Antibody

Phospho-PLCB3 (S537) Antibody

  • 货号：
    CSB-PA000706
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of 293T cells using Phospho-PLC β3 (S537) Polyclonal Antibody
    • Western Blot analysis of 293T AD293 cells using Phospho-PLC β3 (S537) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q01970
  • 基因名：
  • 别名：
    1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody; 1-phosphatidylinositol-4 antibody; 5-bisphosphate phosphodiesterase beta-3 antibody; Phosphoinositide phospholipase C antibody; Phosphoinositide phospholipase C-beta-3 antibody; Phospholipase C beta 3 antibody; Phospholipase C beta 3 (phosphatidylinositol specific) antibody; Phospholipase C-beta-3 antibody; PLC beta 3 antibody; PLC-beta-3 antibody; Plcb3 antibody; PLCB3_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human PLC β3 around the phosphorylation site of S537.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 基因功能参考文献：
    1. Data suggest that 300-residue C-terminal domain of PLCB3 promotes adsorption to phospholipid monolayer/membrane bilayer and is required for spatial organization/adsorption of PLCB3 on membrane surface; defects in phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis alter monolayer adsorption, thus, suggesting role of active site in this process; PLCB3 is preferentially adsorbed to region of bilayer enriched with PIP2. PMID: 28945350
    2. These results indicate that the mechanism by which Galphaq and PLC-beta3 mutually regulate each other is far more complex than a simple, two-state allosteric model and instead is probably kinetically determined. PMID: 28842497
    3. We propose that unliganded PLC-beta exists in equilibrium between a closed conformation observed in crystal structures and an open conformation where the PH domain moves away from the EF hands. Therefore, intrinsic movement of the PH domain in PLC-beta modulates Gbetagamma access to its binding site. PMID: 27002154
    4. the MCP1-induced cortactin phosphorylation is dependent on PLCb3-mediated PKC activation, and siRNA-mediated down-regulation of either of these molecules prevents cortactin interaction with WAVE2 PMID: 26490115
    5. membranes are integral for the activation of PLC-beta isozymes by diverse modulators. PMID: 25193662
    6. the M3 muscarinic receptor maximizes the efficiency of PLCbeta3 signaling beyond its canonical role as a guanine nucleotide exchange factor for Galpha. PMID: 24596086
    7. This study provides an understanding of the structural basis for the PDZ-mediated NHERF1-PLCbeta3 interaction that could prove valuable in selective drug design against CXCR2-related cancers. PMID: 24642259
    8. PLCbeta3 is enriched in the cytosol. PMID: 23006664
    9. phospholipase C-beta3 structure reveals the role of the its distal C-terminal domain PMID: 23377541
    10. Stromal cell-derived factor-1 signaling via the CXCR4-TCR heterodimer uses PLC-beta3 to activate the Ras-ERK pathway and increase intracellular calcium ion concentrations PMID: 21705626
    11. Data demonstrate that PLCB3, by regulating intracellular calcium transients, plays a relevant role in amplifying the expression and release of IL-8, the major chemokine recruiting neutrophils in CF airway lungs. PMID: 21411730
    12. Activation of hPLCbeta3 by U73122 required covalent modification of cysteines as evidenced by the observation that enzyme activation was attenuated by thiol-containing nucleophiles, l-cysteine and glutathione. PMID: 21266572
    13. Studies indicate that the complex between the effector protein phospholipase C-beta3 (PLC-beta3) and its activator, Galpha(q), suggests that several effectors independently evolved a structurally similar helix-turn-helix segment for G protein recognition. PMID: 21304157
    14. study describe the structure of PLC-beta3 in an activated complex with Galphaq, which together with supporting biochemical and physiological analyses reveals its mechanism of transmembrane signaling PMID: 20966218
    15. PLC beta 3 and PLC beta 1 bind to calmodulin PMID: 12821674
    16. findings demonstrated that G-protein-activated phospholipase C-beta interacts with cell polarity proteins Par3 and Par6 to form protein complexes and to mediate downstream signal transduction PMID: 15782111
    17. reduced Ca(2+) signaling in high glucose cannot be explained by reduced Ca(2+) stores but is due to conventional PKC-dependent phosphorylation and inactivation of PLC-beta(3) PMID: 15998840
    18. Homodimerization of PLC-beta3 and PLC-beta1 isoenzymes was observed but heterodimerization of these isoenzymes was not detected. PMID: 16763092
    19. Fission of transport carriers at the trans-Golgi network is dependent on specifically PLCbeta3, which is necessary to activate PKCeta and PKD in that Golgi compartment, via diacylglycerol production. PMID: 17492941
    20. both negative crosstalk from the cAMP/PRKA pathway and a negative feedback loop in the oxytocin/G protein/PLCB pathway involving PRKC operate in myometrial cells PMID: 18322273
    21. PLCbeta3 may provide a selective target for inhibiting Ca(2+) responses to mediators of inflammation, including C5a, UDP, PAF, and LPA PMID: 18411281
    22. VEGF, specifically through VEGFR2, induces phosphorylation of two serine residues on PLCbeta3. PMID: 19295129

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  • 亚细胞定位：
    Cytoplasm. Membrane. Nucleus.
  • 数据库链接：

    HGNC: 9056

    OMIM: 600230

    KEGG: hsa:5331

    STRING: 9606.ENSP00000279230

    UniGene: Hs.523761