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  4. Phospho-OXSR1 (Thr185) Antibody

Phospho-OXSR1 (Thr185) Antibody

  • 货号：
    CSB-PA067482
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from HepG2 cells treated with serum using OSR1 (Phospho-Thr185) Antibody.The lane on the right is treated with the antigen-specific peptide.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody (left)or the same antibody preincubated with blocking peptide (right).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) OXSR1 Polyclonal antibody
  • Uniprot No.：
    O95747
  • 基因名：
    OXSR1
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Peptide sequence around phosphorylation site of threonine185(R-K-T(p)-F-V) derived from Human OSR1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Phosphorylates RELL1, RELL2 and RELT. Phosphorylates PAK1. Phosphorylates PLSCR1 in the presence of RELT.
  • 基因功能参考文献：
    1. Data demonstrate that hypotonic low-chloride conditions that activate the WNK1-SPAK and OSR1 pathway promote phosphorylation of NKCC2 isoforms. PMID: 21321328
    2. OSR1 and SPAK integrate signals from osmosensing and survival pathways. PMID: 24191005
    3. our data show a novel role for the WNK1/OSR1/NKCC1 pathway in glioma migration PMID: 24555568
    4. SPAK and OSR1 are powerful negative regulators of the cell volume regulatory Cl- channel ClC-2 PMID: 25323061
    5. study identifies a separation of functions for the WNK1-activated protein kinases OSR1 and SPAK in mediating proliferation, invasion, and gene expression in endothelial cells PMID: 25362046
    6. The CCT domain directly interacts with the kinase domain to block substrate access and inhibit the domain-swapped homodimerization of the kinase domain of OSR1. PMID: 25389294
    7. WNK 1, 3, 4, OSR1, and SPAK signaling system known to play a role in regulating the phosphorylation status, and hence activity of the CCCs in other tissues, is also present in the rat and human lenses. PMID: 25515571
    8. OSR1 has the capacity to downregulate the peptide transporters PEPT1 and PEPT2 by decreasing the carrier protein abundance in the cell membrane PMID: 25531100
    9. Both, SPAK and OSR1, are negative regulators of the creatine transporter SLC6A8 PMID: 25531585
    10. SPAK and OSR1 are negative regulators of EAAT3 activity PMID: 26112741
    11. SPAK and OSR1 are powerful negative regulators of the excitatory glutamate transporters EAAT1 and EAAT2. PMID: 26233565
    12. SPAK and OSR1 are powerful stimulators of the intestinal Na+-coupled phosphate co-transporter NaPi-IIb PMID: 26506223
    13. OSR1 protein has both the potential to up-regulate KCNQ1/E1 protein abundance in the cell membrane, an effect possibly participating in the regulation of cell volume, excitability, epithelial transport and metabolism. PMID: 26584301
    14. SPAK and OSR1 are both stimulators of Kir2.1 activity. PMID: 26673921
    15. Both SPAK and OSR1 kinases entering cells through exosomes are preferentially expressed at the plasma membrane and that the kinases in exosomes are functional and maintain NKCC1 in a phosphorylated state. PMID: 27122160
    16. the WNK-regulated SPAK/OSR1 kinases directly phosphorylate the N[K]CCs and KCCs, promoting their stimulation and inhibition respectively. PMID: 24393035
    17. SPAK and OSR1, which are often coexpressed in cells, can form functional heterodimers. PMID: 23034389
    18. Data show that intracellular association between WNK1 and oxidative stress-responsive 1 (OSR1) is required for stimulation of OSR1 and Na(+), K(+), Cl(-)-Cotransporter NKCC1 and NKCC2 activities by osmotic stress. PMID: 22989884
    19. OXSR1 and WNK3 transcripts were substantially overexpressed in subjects with schizophrenia relative to comparison subjects. PMID: 20819979
    20. interacts with cation chloride cotransporters PMID: 12386165
    21. isolation and characterization of OSR1 (oxidative stress-responsive 1), one of two human Fray homologs. OSR1 is a 58-kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines PMID: 14707132
    22. WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to cation-chloride-coupled cotransporters PMID: 16263722
    23. OXSR1 kinase is shown to interact with the three RELT family members RELT, RELL1, and RELL2 by in vitro co-immunoprecipitation; additionally, OXSR1 phosphorylates RELT family members in an in vitro kinase assay. PMID: 16389068
    24. data establish that the CCT domain functions as a multipurpose docking site, enabling SPAK/OSR1 to interact with substrates (NKCC1) and activators (WNK1/WNK4) PMID: 16669787
    25. OSR1 and sterile20-related, proline-, alanine-rich kinase are likely links between WNK lysine deficient protein kinase 1 and solute carrier family 12 in a pathway that contributes to volume regulation and blood pressure homeostasis in mammals PMID: 16832045
    26. These results provide the first molecular insight into the mechanism by which the SPAK and OSR1 kinases specifically recognize their upstream activators and downstream substrates. PMID: 17721439
    27. The WNK1-SPAK/OSR1 signalling pathway plays a key role in controlling the phosphorylation and activity of NCC. PMID: 18270262
    28. The first crystal structure of an OSR1 fragment encompassing the catalytic domain of the enzyme, is reported. PMID: 18831043
    29. crystal structure of OSR1 kinase domain has been solved at 2.25 A; OSR1 forms a domain-swapped dimer in an inactive conformation, in which P+1 loop and alphaEF helix are swapped between dimer-related monomers PMID: 19177573

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
  • 组织特异性：
    Ubiquitously expressed in all tissue examined.
  • 数据库链接：

    HGNC: 8508

    OMIM: 604046

    KEGG: hsa:9943

    STRING: 9606.ENSP00000311713

    UniGene: Hs.475970