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  4. Phospho-LIG4 (T650) Antibody

Phospho-LIG4 (T650) Antibody

  • 货号：
    CSB-PA009826
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P49917
  • 基因名：
    LIG4
  • 别名：
    DNA ligase IV ATP dependent antibody; DNA joinase antibody; DNA ligase 4 antibody; DNA ligase IV antibody; DNA repair enzyme antibody; DNLI4_HUMAN antibody; LIG 4 antibody; LIG4 antibody; LIG4S antibody; Ligase IV antibody; Ligase IV DNA ATP dependent antibody; Polydeoxyribonucleotide synthase [ATP] 4 antibody; Polydeoxyribonucleotide synthase 4 antibody; Polydeoxyribonucleotide synthase antibody; Polynucleotide ligase antibody; Sealase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from Human DNA Ligase IV around the phosphorylation site of T650.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. LIG4 regulates nuclear localization of XRCC4.
  • 基因功能参考文献：
    1. Cells doubly deficient in Pol theta; and Lig4 exhibit 100% gene-targeting efficiency because of virtually no random integration events. PMID: 28695890
    2. These studies provide a scaffold for defining impacts of LigIV catalytic core mutations and deficiencies in human LIG4 syndrome. PMID: 29980672
    3. study demonstrated that there was an association between DNA ligase 4 Thr9Ile polymorphism and male infertility and suggests CT genotype as a risk factor for male infertility PMID: 28991497
    4. An increased frequency of binucleated lymphocytes with micronuclei was increased in carriers of the T/T genotype of the LIG4 (rs1805388) gene compared to miners harbouring the C/T genotype. PMID: 28992182
    5. Cellular NHEJ of diverse ends thus identifies the steps necessary for repair through LIG4-mediated sensing of differences in end structure and consequent dynamic remodeling of aligned ends. PMID: 28930678
    6. This study shown that both ligase IV and XRCC4 may act in concert to modulate the development of glioma. PMID: 27508978
    7. We demonstrated an association between six previously published single nucleotide polymorphisms (rs15869 [ BRCA2], rs1805389 [ LIG4], rs8079544 [ TP53], rs25489 [ XRCC1], rs1673041 [ POLD1], and rs11615 [ ERCC1]) and subsequent CNS tumors in survivors of childhood cancer treated by radiation therapy. PMID: 28976792
    8. In a recombinant PNKP-XRCC4-LigIV complex, both the PNKP FHA and catalytic domains contact the XRCC4 coiled-coil and LigIV BRCT repeats. Multipoint contacts between PNKP and XRCC4-LigIV regulate PNKP recruitment and activity within NHEJ. PMID: 28453785
    9. Data suggest that stimulation of Artemis nuclease/DCLRE1C activity by XRCC4-DNA ligase IV hetero-complex and efficiency of blunt-end ligation are determined by structural configurations at the DNA ends. (XRCC4 = X-ray repair cross complementing 4) PMID: 28696258
    10. found that the rs228593, rs2267437 and rs1805388 functional polymorphisms probably alter the level of expression of the ATM, XRCC6 and LIG4 genes, respectively, being important in the maintenance of genomic instability in MDS PMID: 27497341
    11. marked overlap of dyskeratosis congenita with four other genetic syndromes, confounding accurate diagnosis and subsequent management. Patients with clinical features of dyskeratosis congenita need to have genetic analysis of USB1, LIG4 and GRHL2 in addition to the classical dyskeratosis congenita genes and telomere length measurements. PMID: 27612988
    12. The rs1805388 in LIG4 was associated with increased radioresistance. PMID: 26974709
    13. LIG4 is highly upregulated in human colorectal cancer cells. PMID: 27009971
    14. Five novel mutations in LIG4 and a potential hotspot mutation (c.833G > T; p.R278L) in the Chinese population with LIG4 deficiency syndrome were identified. PMID: 26762768
    15. Our study identifies LIG4 as a predictor of an increased risk for early biochemical recurrence in prostate cancer PMID: 26134445
    16. The genetic polymorphisms in LIG4 rs1805388 and HSPB1 rs2868371 were not obviously correlated with the risk of radiation pneumonitis and radiation-induced lung injury of lung cancer. PMID: 25811031
    17. our results suggest that LIG4 rs10131 polymorphism in the DNA repair pathways plays an important role in the risk of glioma in a Chinese population. PMID: 25973104
    18. Our data showed that Cd altered the phosphorylation of DNA-PKcs, and reduced the expression of both XRCC4 and Ligase IV in irradiated cells. PMID: 26201248
    19. despite several limitations, this meta-analysis suggested that LIG4 T9I genetic variant is associated with a decreased risk of cancer among Caucasians, however, the rs1805386 gene polymorphism is not a risk factor of cancer. PMID: 25314918
    20. High LIG4 expression is associated with less radiosensitivity of nasopharyngeal cancer. PMID: 25605214
    21. DNA Ligase IV is required for efficient localization of XRCC4 and XLF into cell nucleus. PMID: 24984242
    22. Ku-independent, LIGIV-dependent repair pathway exists in human somatic cells. PMID: 24837021
    23. LIG4 Thr9Ile polymorphism is associated with treatment response in advanced non-small cell lung cancer. PMID: 24722796
    24. Our data did not show association between LIG4 and RAD52 SNPs and SLE, its clinical manifestations or ethnicity in the tested population. PMID: 24415301
    25. Loss of LIG4 is associated with colorectal cancer. PMID: 24282031
    26. Results show that in cells deficient for Lig4, chromosomal translocations junctions had significantly longer deletions and more microhomologies. PMID: 25201414
    27. Characterization of the protein interaction domains that modulate the XRCC4/Ligase IV interaction. PMID: 23794378
    28. study reports the identification of biallelic truncating LIG4 mutations in 11 patients with microcephalic primordial dwarfism presenting with restricted prenatal growth and extreme postnatal global growth failure PMID: 24123394
    29. DNA ligase IV expression of K562/DNR was also suppressed significantly with Sp1 family protein inhibition PMID: 24530422
    30. DNA ligase IV and Artemis act cooperatively to promote nonhomologous end-joining PMID: 23967291
    31. These results indicate for the first time that LIG4 rs1805388 and X-ray Repair Cross Complementing-4 (XRCC4) rs1805377, alone or in combination, are associated with a risk of gliomas. PMID: 23663450
    32. The chromatin binding of XRCC4 was dependent on the presence of LIG4. PMID: 23994631
    33. genetic association studies in population in China: Data suggest that SNPs in LIG4 (rs1805388, exon 2 54C>T, Thr9Ile) and RAG1 (recombination activating gene 1, rs2227973, A>G, K820R) are associated with male infertility. PMID: 23630330
    34. No association between LIG4 gene polymorphisms (rs1805386 T>C, rs1805389 C>T, rs1805388 C>T and rs2232641 A>G) and breast cancer risk. PMID: 22994770
    35. Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. PMID: 23219551
    36. DNA Ligase IV is differentially required for certain chromosome fusion events induced by telomere dysfunction. PMID: 23275564
    37. The flexibility of the DNA ligase IV catalytic region is limited in a manner that affects the formation of the LigIV/XRCC4/XLF-Cernunnos complex. PMID: 22658747
    38. The alpha2 helix in the Lig4 BRCT-1 domain is required for adenovirus-mediated degradation of Lig4. PMID: 22534089
    39. polymorphisms in LIG4 do not contribute to cancer risk in a population of Lynch syndrome patients with colorectal cancer PMID: 21974800
    40. XRCC4 modulates the dynamic interaction of the Ligase IV/XRCC4 complex with the NHEJ machinery at double-stranded DNA breaks PMID: 21982441
    41. The current results indicated that NHEJ genetic polymorphisms, particularly LIG4 rs1805388, may modulate the risk of RP in patients with NSCLC who receive definitive radio(chemo)therapy PMID: 21717429
    42. these results suggest that some variants in XRCC4, LIG4 and Ku80 genes can contribute to thyroid cancer susceptibility PMID: 20811692
    43. in the Chinese population, LIG4 Ile658Val has only a slight impact on the risk of developing cervical carcinoma PMID: 20400235
    44. Polymorphisms in the LIG4, BTBD2, HMGA2, and RTEL1 genes, which are involved in the double-strand break repair pathway, are associated with glioblastoma multiforme survival. PMID: 20368557
    45. role of NHEJ enzyme Ligase IV in the pathogenesis of MDS PMID: 19727724
    46. provide a first sight of the structural organization of the Lig4-Xrcc4 complex, which suggests that the BRCT domains could provide the link of the ligase to Xrcc4 while permitting some movements of the catalytic domains of Lig4. PMID: 19837014
    47. crystal structure of an Xrcc4-DNA ligase IV complex PMID: 11702069
    48. mutations identified in patients exhibiting developmental delay and immunodeficiency PMID: 11779494
    49. Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. PMID: 12077346
    50. Genetic variants of NHEJ DNA ligase IV can affect the risk of developing multiple myeloma, a tumour characterised by aberrant class switch recombination PMID: 12471202

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  • 相关疾病：
    LIG4 syndrome (LIG4S); Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ATP-dependent DNA ligase family
  • 组织特异性：
    Testis, thymus, prostate and heart.
  • 数据库链接：

    HGNC: 6601

    OMIM: 601837

    KEGG: hsa:3981

    STRING: 9606.ENSP00000349393

    UniGene: Hs.166091