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  4. Phospho-KIF20A (S528) Antibody

Phospho-KIF20A (S528) Antibody

  • 货号：
    CSB-PA006385
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of 293 cells using Phospho-KIF20A (S528) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    O95235
  • 基因名：
    KIF20A
  • 别名：
    AA415432 antibody; FLJ21151 antibody; GG10 2 antibody; GG10_2 antibody; KI20A_HUMAN antibody; KIF20A antibody; kinesin family member 20A antibody; Kinesin like protein KIF20A antibody; Kinesin-like protein 174 antibody; Kinesin-like protein KIF20A antibody; mitotic kinesin like protein 2 antibody; Mitotic kinesin-like protein 2 antibody; MKlp2 antibody; OTTHUMP00000159481 antibody; RAB6 interacting; kinesin like (rabkinesin6) antibody; Rab6-interacting kinesin-like protein antibody; RAB6-INTERACTING PROTEIN; KINESIN-LIKE antibody; RAB6KIFL antibody; Rabkinesin 6 antibody; Rabkinesin-6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from Human KIF20A around the phosphorylation site of S528.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
  • 基因功能参考文献：
    1. KIF20A-knockdown exhibits a suppressive effect on the cell cycle and has an inhibitory effect on the terminal cell division process (cytokinesis) leading to partial multinuclearity of the cells. PMID: 30182171
    2. In conclusion, our findings indicated that FOXM1 was highly expressed in lung cancer cells after exposure to ionizing radiation (IR). We also found that FOXM1 promoted radioresistance, invasion, migration, and EMT of lung cancer cells after IR, partly through upregulating KIF20A. PMID: 29704495
    3. Although the present study was preliminary, these data indicate the possible involvement of KIF20A in the proliferation of clearcell carcinoma (CCC), suggesting that targeting this molecule may contribute to reversing the malignant potential consequently affecting the oncologic outcome of CCC patients. PMID: 29749467
    4. Results suggest that coupling between actin and microtubule cytoskeletons driven by Myosin II and KIF20A ensures the spatial coordination between RAB6-positive vesicles fission from Golgi/trans-Golgi network membranes and their exit along microtubules. PMID: 29093437
    5. Study reveals the mechanism controlling abscission through integration of Aurora B kinase and B56-bound PP2A phosphatase activities on the kinesin motor protein MKlp2. MKlp2 is an essential protein for promoting abscission, which may regulate tethering and stabilizing of the PM to the microtubule cytoskeleton at the intercellular bridge through its previously uncharacterized lipid association motif. PMID: 27939310
    6. Identification of a novel autosomal recessive congenital restrictive cardiomyopathy, caused by a near complete loss-of-function of KIF20A. PMID: 29357359
    7. While the MKLP2 neck-linker is directed towards the microtubule plus-end in an ATP-like state, it does not fully dock along the motor domain. Furthermore, the footprint of the MKLP2 motor domain on the microtubule surface is altered compared to motile kinesins, and enhanced by kinesin-6-specific sequences. PMID: 28826477
    8. High KIF20A expression is associated with hepatocellular carcinoma. PMID: 27036048
    9. Study shows that the kinesin family member 20A (KIF20A) is a tumor-associated antigen involved in the glioma cell growth and cell survival, suggesting that KIF20A is an oncoantigen of gliomas. PMID: 28070829
    10. KIF20A is overexpressed and may serve as an independent prognostic biomarker in Nasopharyngeal Cancer. Targeting KIF20A reduces migration and invasion of Nasopharyngeal Cancer cells. PMID: 28081138
    11. elevated KIF20A expression correlates with HPV infection, clinical stage, tumor recurrence, lymphovascular space involvement, pelvic lymph node metastasis, and poor outcome in early-stage cervical squamous cell carcinoma patients. PMID: 27941992
    12. we identify KIF20A as a direct transcriptional target of FOXM1, involved in paclitaxel action and resistance PMID: 25961928
    13. Our results provide insight into the link between regulation of KIF20A-mediated trafficking of IGF2BP3-containing stress granules and modulation of the motility and invasiveness in pancreatic cancers. PMID: 25499221
    14. Kif20a expression was upregulated in pancreatic ductal adenocarcinoma and pancreatic neuroendocrine cancer compared with normal pancreas. Kif20a silencing resulted in inhibition of proliferation, motility, and invasion of pancreatic cancer cell lines. PMID: 25953216
    15. s propose that phosphoregulation of MKlp2 by Cdk1/cyclin B1 ensures that activation of MKlp2 kinesin and relocation of the CPC occur at the appropriate time and space for proper mitotic progression and genomic stability. PMID: 24656812
    16. targeting Aurora B to the cell cortex (or the equatorial cortex) by MKlp2 is essential for the maintenance of the ingressing furrow for successful cytokinesis PMID: 23750214
    17. Primary melanomas that were positive for KIF20A showed a significantly greater thickness than those that were negative, and patients with KIF20A+ melanoma tended to develop recurrence earlier. PMID: 22854760
    18. Used proteomics to identify the genistein-induced protein alterations in gastric cancer cells and found the silencing of KIF20A inhibited cell viability and induced G2/M arrest,& also increased cancer cell sensitivity to genistein inhibition. PMID: 22887948
    19. This gene is not involved in Charcot-Marie-Tooth disease. PMID: 12469216
    20. Collaboration of RAB6KIFL and disc large homologue 5 is likely to be involved in pancreatic cancer. PMID: 15665285
    21. Data demonstrate that Mklp2 and the chromosomal passenger complex mutually depend on each other for microtubule midzone localization, and that the association between the CPC and Mklp2 is negatively regulated by Cdk1. PMID: 19303298
    22. KIF20A (Rab6KIFL/MKlp2) is required for cytokinesis PMID: 11060022
    23. Relocation of the Aurora B/INCENP/survivin passenger protein complex requires KIF20A (MKlp2). PMID: 15263015
    24. KIF20A (MKlp2) is required for targeting of the polo like kinase 1 during anaphase and telophase cells. PMID: 12939256

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  • 亚细胞定位：
    Golgi apparatus. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
  • 数据库链接：

    HGNC: 9787

    OMIM: 605664

    KEGG: hsa:10112

    STRING: 9606.ENSP00000378356

    UniGene: Hs.718626