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  4. Phospho-IL10RA (Tyr496) Antibody

Phospho-IL10RA (Tyr496) Antibody

  • 货号：
    CSB-PA051687
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts HUVEC tissue using IL-10R alpha (Phospho-Tyr496) antibody.The lane on the right is treated with the antigen-specific peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) IL10RA Polyclonal antibody
  • Uniprot No.：
    Q13651
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence around phosphorylation site of Tyrosine496 (K-G-Y(p)-L-K) derived from Human IL-10R alpha.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
  • 基因功能参考文献：
    1. This case report and case-control study explores the role of IL10RA and its deficiency in the development of the immune system and inflammatory bowel diseases. PMID: 28644354
    2. We identified 32 compound heterozygous mutations and 9 homozygous mutations in IL10 receptor subunit alpha and 1 homozygous mutation in IL10 receptor subunit beta. Among these mutations, 10 novel mutations were identified, and 6 pathogenic mutations had been previously described. In patients with IL10 receptor subunit alpha mutations, c.301C>T (p.R101RW) and c.537 G>A (p.T179T) were the most common mutations. PMID: 28267044
    3. these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2 PMID: 28893958
    4. Expression of IL10R subunits within the leukocyte population (CD45(+) cells) was significantly higher in primary brain tumors than in metastases. PMID: 28982901
    5. This study showed that lack of association with schizophrenia was detected for IL10 and IL10RA single polymorphisms and haplotypes. PMID: 27397081
    6. The IL-10RA rs9610 A allele was increased in rheumatoid arthritis (RA) patient group compared with control subjects. Interestingly, significant differences were detected both in the allele and genotype frequencies of rs9610 between anti-CCP (anti-cyclic citrullinated peptide) positive patients and anti-CCP negative patients. The findings suggest that IL-10RA rs9610 polymorphism might contribute to RA susceptibility. PMID: 27796662
    7. These results suggest that S138G loss-of-function polymorphism of the IL-10R1 may be important risk factor in increasing susceptibility to multiple sclerosis. PMID: 28225209
    8. Results reveal the structure of box1 from class II cytokine receptors IFNLR1 and IL10RA bound to the FERM-SH2 domain of human JAK1, identifying a consensus motif for JAK1 interaction. PMID: 27133025
    9. a JAK2 Inhibitor Suppresses a BCL6-dependent IL10RA/JAK2/STAT3 Pathway in High Grade B-cell Lymphoma. PMID: 27268052
    10. genetic association study in cohort of infants/children: Data suggest perianal fistulas are exhibited in patients with very early-onset inflammatory bowel disease with IL10 receptor mutations (IL10RA/B); prognosis and response to treatment are poor. PMID: 25373860
    11. IL-10Ralpha expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma PMID: 26631117
    12. SNP3 polymorphism is associated with myocardial infarction; may generate some conformational rearrangements of the IL-10R1 receptor domain which affects IL-10 complex binding and alters its downstream signal PMID: 24566517
    13. High IL10RA expression is associated with diffuse large B-cell lymphoma. PMID: 25733167
    14. Inflammatory bowel disease (IBD) in infancy is phenotypically and genetically different disease entity from adult-onset or older child-onset IBD. It has a strong association with IL-10 receptor gene. PMID: 24785691
    15. IL10R1 loss-of-function A536/S138G polymorphism may contribute to recurrent pregnancy loss pathogenesis PMID: 24689510
    16. Very early onset inflammatory bowel disease associated with aberrant trafficking of IL-10R1 and cure by T cell replete haploidentical bone marrow transplantation. PMID: 24519095
    17. Mutations in the IL10RA gene is associated with ulcerative colitis. PMID: 24216686
    18. Case Report: pediatric ulcerative colitis with a novel point mutation within the IL10RA promoter (the -413G->T), inherited from his mother. PMID: 24379584
    19. This report confirms the genetic defect of IL-10RA in neonatal-onset inflammatory bowel disease. PMID: 23839161
    20. IFN-gamma selectively induced the expression of IL-10R1 on intestinal epithelia, predominantly on the apical membrane of polarized epithelial cells. PMID: 24367025
    21. A mutation in TLR4 (rs4986790) and IL10RA (rs22291130) was significantly associated with Mycobacterium avium subspecies paratuberculosis-positive Crohn's disease patients. PMID: 23455702
    22. 5 patients with an IL-10R1 or IL-10R2 deficiency developed B-cell non-Hodgkin lymphoma between the ages of 5 and 6 years (which was recurrent in 1 patient). PMID: 24089328
    23. IL10RA polymorphisms are associated with ulcerative colitis. PMID: 22550014
    24. In the Chinese Han population, missense SNPs within the exons of the IL-10R1 gene do not contribute to the development of systemic lupus erythematosus PMID: 22652629
    25. The results suggest that genetic polymorphisms in TNF and IL10RA genes may modify the association between blood transfusion and NHL risk. PMID: 22649007
    26. IL-10R1 is a novel substrate of betaTrCP-containing ubiquitin E3 ligase, a novel negative regulatory mechanism that may potentially affect IL-10 function PMID: 22087322
    27. We found an IL10R variant, which may be associated with a decreased response to the cytokine in one patient. PMID: 22155628
    28. IL-10R1 S138G loss-of-function polymorphism is associated with extrapulmonary tuberculosis risk development in Tunisia. PMID: 21553229
    29. The haplotype -185/-116 of IL10 receptor alpha in combination with the haplotype -754/-750 of IL10 receptor beta contributed towards mild malaria. PMID: 21814839
    30. IL10R1-G330R does not alter surface expression but duration of STAT phosphorylation, indicating that the position of G330 is important in stabilizing the STAT signal. PMID: 21654841
    31. linkage disequilibrium (LD) blocks were formed in IL10 and IL10RA PMID: 21532858
    32. IL-10R1 expression on CD4+ T cells and signalling in PBMCs were down-regulated in lupus nephritis (LN) patients, indicating that IL-10 and its receptor may have a special role in LN pathogenesis PMID: 21635228
    33. Besides overall immune health, host genetic factors influence mortality, retinitis progression, and retinal detachment in patients with AIDS and CMV retinitis that are receiving HAART. PMID: 21396623
    34. in the Lebanese population, the loss-of-function allele IL-10R1-S138G (SNP3) is unlikely to provide a protective effect against ulcerative colitis and that both IL-10R1 variants do not correlate with inflammatory bowel disease PMID: 20186944
    35. G carriers for the -536AG IL-10R1 gene polymorphism had higher systolic and diastolic pressures, and IL-10 levels; and obese G carriers had an increased waist-to-hip ratio. PMID: 19798061
    36. cmvIL-10 forms a disulfide-linked homodimer that binds two sIL-10R1 molecules. PMID: 12093920
    37. IL-10 receptor stimulated the rapid translocation of IL-10E1 to the cell nucleus and the activation of TIMP-1 expression in primary human prostate tumor cell lines. PMID: 12496489
    38. Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells. PMID: 12591723
    39. This investigation reveals three previously unrecognized polymorphisms of IL-10R1 (SNP3, SNP4, and SNP5), two of which result in an amino acid substitution; substitution in S138G variant may interfere with binding of IL-10 to IL-10R1. PMID: 12759436
    40. Homozygosity of the IL-10R1 G330R allele is associated with schizophrenia and may contribute to the expression of disease phenotype in susceptible individuals. PMID: 17066477
    41. Genetic variation in IL-10RA/IL-22 genes may play a modulatory role in the outcome of hepatitis C infection. PMID: 17845543
    42. The IL-4R Ile50/Ile50 and IL-10R2 G520/G520 and G520/A520 genotypes were shown to determine the susceptibility to SLE (systemic lupus erythematosus)in a Chinese population PMID: 17988330
    43. The peripheral blood neutrophils of septic patients constitutively display abundant levels of surface IL-10R1. PMID: 18308712
    44. IL-10R is associated with the progression of the renal cell carcinoma PMID: 18344594
    45. Data demonstrate a significant relation between cervical concentrations of IL-10 and single nucleotide polymorphisms in the IL-10 receptor alpha and beta genes. PMID: 18674658
    46. The IL-10R1 S138G loss-of-function allele and ulcerative colitis are reported. PMID: 18800073
    47. a variant rs17121510 in the interleukin-10 receptor antagonist (IL-10RA) gene for allele (p = 0.01) and genotype (p = 3.34x10(-4)) may have a role in preterm birth PMID: 18818748
    48. IL-10R1 variants differentially reduce the signaling activity of cmvIL-10 PMID: 19016528
    49. Mutations in genes encoding the IL10R subunit proteins were found in patients with early-onset enterocolitis, involving hyperinflammatory immune responses in the intestine. PMID: 19890111
    50. The capacity of neutrophils to respond to IL-10, as assessed by Stat3 tyrosine phosphorylation, SOCS-3 expression, and modulation of cytokine production, is dependent on the level of expression of IL-10R1. PMID: 11490020

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  • 相关疾病：
    Inflammatory bowel disease 28 (IBD28)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cytoplasm.
  • 蛋白家族：
    Type II cytokine receptor family
  • 组织特异性：
    Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.
  • 数据库链接：

    HGNC: 5964

    OMIM: 146933

    KEGG: hsa:3587

    STRING: 9606.ENSP00000227752

    UniGene: Hs.504035