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  4. Phospho-FOXO4 (Ser197) Antibody

Phospho-FOXO4 (Ser197) Antibody

  • 货号：
    CSB-PA591269
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody(left) or the same antibody preincubated with blocking peptide(right).
    • Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) FOXO4 Polyclonal antibody
  • Uniprot No.：
    P98177
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • 基因功能参考文献：
    1. Study reports that CK1alpha similarly destabilizes FOXO4 in RAS-mutant cells by phosphorylation at serines 265/268. PMID: 28945225
    2. In this review, we introduce the regulation of FOXO4 in physiological and pathological conditions. Particularly, the pathophysiological processes and molecular pathways regulated by FOXO4 in the development and progression of cancer are also summarized PMID: 29719213
    3. The results of this genomic analysis suggest that low FOXO4 expression is a significant risk factor for epileptic seizures in patients with LGGs and is associated with the seizure outcome. PMID: 28963932
    4. NF-kappaB/snail/YY1/RKIP circuitry regulated by FOXO4 were likely involved in miR-150-induced EMT event. PMID: 27976702
    5. Negative expression of FoxO3/FoxO4 and lymph node metastasis were the risk factors for the poor prognosis of bladder cancer. PMID: 28554751
    6. Knockdown of FOXO4 but not FOXO1 expression decreased proteasome activity. Following neural differentiation, the HD-iPSC-derived neural progenitor cells (NPCs) demonstrated lower levels of proteasome activity and FOXO expressions than their WT counterparts. More importantly, overexpression of FOXO4 but not FOXO1 in HD NPCs dramatically enhanced proteasome activity. PMID: 28973411
    7. Results show that a small subset of lymphoma cells surviving treatment with doxorubicin or phenylbutyrate showed stem cell-like properties and resistance to chemotherapeuty. The overexpression of FOXO4 was found in these surviving cells, and DLBCL patients with FOXO4-positive tumor cells had poor prognosis. PMID: 27911272
    8. FOXO4 and FOXD3 were shown independently predictive of overall survival in gastric cancer PMID: 27027443
    9. FoxO1 and FoxO4 antagonize Tat-mediated transactivation of HIV-1 promoter through the repression of Tat protein expression. PMID: 28699853
    10. FOXO4 has an inhibitory effect in clearcell renal carcinoma cells, at least in part through inducing apoptosis via upregulation of Bim in the mitochondria-dependent pathway. PMID: 26780985
    11. knockdown of Ku70 inhibited cell proliferation accompanying an increase in p27(kip1) levels through interacting with FOXO4 PMID: 26797321
    12. miR-664 functions as an oncogene miRNA and has an important role in promoting human osteosarcoma cell proliferation by suppressing FOXO4 expression. PMID: 26463624
    13. The data demonstrated that elevated miR-150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell growth and survival. PMID: 26715362
    14. Porphyromonas gingivalis-induced reactive oxygen species activate FOXO transcription factors through JNK signalling, and that FOXO1 controls oxidative stress responses, inflammatory cytokine production and cell survival. PMID: 25958948
    15. Cox regression analysis indicated FoxO4 to be an independent prognostic factor in non-small cell lung cancers and suggested that FoxO4 might inhibit the process of EMT in non-small cell lung cancers, and might therefore be a target for therapy. PMID: 24935588
    16. FOXOs support the metabolic requirements of normal and tumor cells by promoting IDH1 expression. PMID: 25648147
    17. data strongly suggest that increased PI3K/AKT-mediated metastatic invasiveness in CaP is associated with FOXO4 loss, and that mechanisms to induce FOXO4 re-expression might suppress CaP metastatic aggressiveness. PMID: 24983969
    18. study demonstrated that miR-1274a prompted gastric cancer cells growth and migration through dampening FOXO4 expression thus provided a potential target for human gastric cancer therapy PMID: 25753202
    19. Data indicate that glycogen synthase kinase 3 beta (GSK3beta) and transcription factors FOXO1/3/4 promote hepatoma cell proliferation through type I insulin-like growth factor receptor (IGF-IR). PMID: 25053419
    20. FOXO4KD-EPCs injected into the rat ischemic limb brought less apoptosis and more neovascularization than EPCs PMID: 24663349
    21. Case Report: CIC-FOXO4 fusion sarcoma is a new type of Ewing-like sarcoma that has a specific genetic signature. PMID: 25007147
    22. Our data suggest that loss of FOXO4 expression contributes to gastric cancer growth and metastasis, and it may serve as a potential therapeutic target for gastric cancer. PMID: 24886657
    23. Data show that forkhead transcription factor 4 (FoxO4) interacts with sterol regulatory element binding protein (SREBP)2 and hypoxia inducible factor (HIF)2alpha to modulate lanosterol 14alpha demethylase (CYP51) promoter activity. PMID: 24353279
    24. Data show that small GTPase RALA regulates formation of a JIP1 (C-Jun-amino-terminal-interacting protein 1) scaffold complex to propagate JNK signaling toward FOXO4 (forkhead box O transcription factor) in response to reactive oxygen species (ROS). PMID: 23770673
    25. We find that loss of FOXO4 reduces the potential of hESCs to differentiate into neural lineages. PMID: 23551888
    26. Taken together, our findings not only suggest that miR-421 promotes nasopharyngeal carcinoma cell proliferation and anti-apoptosis, but also uncover a novel regulatory mechanism for inactivation of FOXO4 in nasopharyngeal carcinoma. PMID: 23707940
    27. Coding mutations within the FOXO4 gene are not associated with premature ovarian failure in women from the Tunisian population. PMID: 22285440
    28. demonstrated that adiponectin activated 5'-AMP-activated protein kinase alpha2 isoform, leading to inhibition of mammalian target of rapamycin complex 1 and S6K1. This in turn stabilized insulin receptor substrate-1, driving Akt2-mediated inhibition of FoxO4 PMID: 21454807
    29. Inhibition of endogenous FOXO proteins attenuated tetradecanoylphorbol Acetate/PDGF-BB mediated differentiation of neuroblastoma cells. PMID: 22411791
    30. Data suggest that expression of cytoplasmic FoxO4 in placenta, fetal membranes, and decidua is altered by parturition/labor, preterm chorioamnionitis, and pro-inflammatory stimuli; silencing of FoxO4 gene initiates apoptosis in placental cell lines. PMID: 22112832
    31. Studies indicate that FoxO1, 3 and 4 genes were discovered at the chromosomal breakpoints found in cancers and were initially implicated in cancer. PMID: 21613825
    32. identified FOXO4 and PDCD4 as direct and functional targets of miR-499-5p PMID: 21934092
    33. FOXO4 may function as a tumor suppressor in the development and progression of colorectal cancer. PMID: 22125836
    34. Studies indictet that the mammalian FoxO family consists of FoxO1, 3, 4 and 6 and are regulated by by AKT and 14-3-3 proteins. PMID: 21708191
    35. gene study of FOXO4, reveals no association with human longevity in Germans PMID: 21388494
    36. Foxo4 may be a useful target for suppression in the treatment of HBV-associated hepatocellular carcinoma cells. PMID: 21567078
    37. DEPP is regulated at the level of transcription by FoxO in human vascular endothelial cells PMID: 21510935
    38. Tax induces a dose-dependent degradation of FoxO4 by the ubiquitin-proteasome pathway. PMID: 21525355
    39. greater in fetal membranes obtained from the supracervical compared to distal site PMID: 20934750
    40. structure of the FOXO4-DNA-binding domain (DBD)-DNA complex suggests that both direct water-DNA base contacts and the unique water-network interactions contribute to FOXO-DBD binding to the DNA in a sequence-specific manner PMID: 21123876
    41. a conserved critical Ku70 role for FOXO function toward coordination of a survival program PMID: 20570964
    42. Findings show that oxidative stress and FOXO4 induce PAI-1 expression through modulation of HIF-1alpha and CREB protein levels and that enhanced CREB binding to the PAI-1 promoter is critical for the PAI-1 induction under oxidative stress. PMID: 20136501
    43. FoxO4 acts on CYP51 to regulate the late steps of cholesterol biosynthesis. PMID: 20037138
    44. PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4. PMID: 20348951
    45. these data provide a mechanism of FOXO4 anti-oxidative protection through O-GlcNAcylation. PMID: 19932102
    46. The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. PMID: 11777915
    47. AFX zeta is a downstream target of both the phosphatidylinositol 3-kinase/PKB insulin signaling pathway and an AMP-activated protein kinase-dependent pathway. PMID: 11779849
    48. Control of cell cycle exit and entry by protein kinase B-regulated forkhead transcription factors PMID: 11884591
    49. the MLL-AFX fusion protein requires the transcriptional effector domains of AFX to transform myeloid progenitors and interfere with forkhead protein function PMID: 12192052
    50. FOXO4 induces the down-regulation of hypoxia-inducible factor 1 alpha by a von Hippel-Lindau protein-independent mechanism PMID: 12761217

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  • 相关疾病：
    A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with KMT2A/MLL1. The result is a rogue activator protein.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • 组织特异性：
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • 数据库链接：

    HGNC: 7139

    OMIM: 300033

    KEGG: hsa:4303

    STRING: 9606.ENSP00000363377

    UniGene: Hs.584654