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Phospho-DOK2 (Tyr299) Antibody

  • 货号：
    CSB-PA935588
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody(left) or the same antibody preincubated with blocking peptide(right).
    • Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody.
    • Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DOK2 Polyclonal antibody
  • Uniprot No.：
    O60496
  • 基因名：
    DOK2
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • 基因功能参考文献：
    1. In this report, the s demonstrated that the cellular adaptor proteins Dok-2 and Dok-1 are tyrosine phosphorylated upon herpes simplex virus 1 infection. In addition, herpes simplex virus 1 induced the selective degradation of Dok-2. Finally, Dok-2 interacts with herpes simplex virus 1 VP11/12, and that herpes simplex virus 1-induced tyrosine phosphorylation and degradation of Dok-2 require VP11/12. PMID: 28841444
    2. DOK2 and DOK3 expression was significantly reduced in HTLV-1-infected T cells. PMID: 27265473
    3. point mutations in DOK1 and DOK2 genes are detected with low frequency in chronic myelomonocytic leukemia but may have consequences for the function of the DOK2 PTB domain PMID: 25252871
    4. Taken together, these results reveal that Dok1 and Dok2 proteins are involved in an intrinsic negative feedback loop downstream of natural killer-cell-activating receptors in mouse and human. PMID: 24963146
    5. DOK2 as a tumor suppressor in EGFR-mutant lung adenocarcinoma PMID: 24255704
    6. DOK2 is a marker of poor prognosis in patients with gastric cancer after curative resection PMID: 22130622
    7. Data indicate there was no evidence of DOK2 somatic mutation in the leukemias analyzed. PMID: 21329978
    8. data indicate that DOK2 is altered in gastric (GC) and colorectal cancers (CRC) by loss of expressions; data indicate that somatic mutation of DOK2 may be rare in GC, CRC, breast cancer, prostate cancer and liver cancer PMID: 21749457
    9. Dok1 negatively regulates Dok2-mediated CD200R signaling through the recruitment of CrkL. PMID: 21078907
    10. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
    11. identified tyrosine residue 1106 on Tie2 as an Angiopoietin 1-dependent autophosphorylation site that mediates binding and phosphorylation of the downstream-of-kinase-related (Dok-R) docking protein PMID: 12665569
    12. Dok-R and c-Abl interact in both a constitutive and inducible fashion and Dok-R influences the intracellular kinase and biological activity of c-Abl PMID: 12777393
    13. Dok-2 tyrosine phosphorylation was also found to be involved in collagen receptor, glycoprotein VI (GPVI), signaling as well as in outside-in signaling through the major platelet integrin, alpha IIIb beta 3 PMID: 14645010
    14. DOK1 and DOK2 interact with the Tec protein tyrosine kinase. PMID: 14647425
    15. Dok-R acts as an EGFR-recruited scaffolding molecule that processively assembles c-Src and Csk to attenuate signaling from the EGFR. PMID: 15831486
    16. Phosphotyrosine-binding mediated oligomerization of Dok-1 and Dok-2 represents an essential step for Dok phosphorylation and function. PMID: 16177091
    17. The data provide evidence that DOK2 protein has a role in regulating cell proliferation and differentiation and is positive regulators of the MAPK signaling pathway in this context. PMID: 16823827
    18. Dok-2 is a critical element of a linker for activation of T cells (LAT)-dependent negative feedback loop that attenuates early T-cell receptor (TCR) signal. PMID: 17043143
    19. results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3 PMID: 17092301
    20. Dok-1/Dok-2 pleckstrin homology domains bind in vitro to the rare phosphoinositide species, phosphatidylinositol 5-phosphate PMID: 19299694
    21. CD200R inhibits the activation of human myeloid cells through direct recruitment of Dok2 and subsequent activation of RAS p21 protein activator 1. PMID: 19786546

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  • 蛋白家族：
    DOK family, Type A subfamily
  • 组织特异性：
    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • 数据库链接：

    HGNC: 2991

    OMIM: 604997

    KEGG: hsa:9046

    STRING: 9606.ENSP00000276420

    UniGene: Hs.71215