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  4. Phospho-DAPK2 (S318) Antibody

Phospho-DAPK2 (S318) Antibody

  • 货号：
    CSB-PA008412
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q9UIK4
  • 基因名：
  • 别名：
    DAK2 antibody; DAP kinase 2 antibody; DAP kinase related protein 1 antibody; DAP kinase related protein 1 beta isoform antibody; DAP-kinase-related protein 1 antibody; DAPK2 antibody; DAPK2_HUMAN antibody; Death associated protein kinase 2 antibody; Death-associated protein kinase 2 antibody; DRP 1 antibody; DRP-1 antibody; DRP1 antibody; MGC119312 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human DAPK2 around the phosphorylation site of S318.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Regulates granulocytic motility by controlling cell spreading and polarization.; Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.
  • 基因功能参考文献：
    1. study reveals a unique calmodulin-independent mechanism for DAPK2 activation, critical to its function as a novel downstream effector of AMPK in autophagy PMID: 29717115
    2. This study suggests that miR-520g contributes to tumor progression and drug resistance by post-transcriptionally downregulating DAPK2 in patients with epithelial ovarian cancer PMID: 27049921
    3. Thyroid hormone promotes selective autophagy via induction of DAPK2-SQSTM1 cascade, which in turn protects hepatocytes from diethylnitrosamine-induced hepatotoxicity or carcinogenesis. PMID: 27653365
    4. miR-520h suppresses Death-associated protein kinase 2 (DAPK2) expression, as restoring DAPK2 abolished miR-520h-promoted drug resistance, and knockdown of DAPK2 mitigated cell death caused by the depletion of miR-520h. PMID: 25982274
    5. that Death-associated protein kinase 2 effector functions are influenced by the protein's subcellular localization PMID: 26483415
    6. This study links adipocyte expression of an autophagy-regulating kinase, lysosome-mediated clearance and fat cell lipid accumulation; it demonstrates obesity-related attenuated autophagy in adipocytes, and identifies DAPK2 dependence in this regulation. PMID: 26038578
    7. DAPK2 is a novel kinase of mTORC1 and is a potential new member of this multiprotein complex, modulating mTORC1 activity and autophagy levels under stress and steady-state conditions. PMID: 25361081
    8. DAPK2 regulates oxidative stress in cancer cells by preserving mitochondrial function PMID: 25741596
    9. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
    10. DAPK2 is upregulated in uterosacral ligaments in pelvic organ prolapse PMID: 23700042
    11. The defect in chemotaxis in DAPK2-inactive granulocytes is likely a result of reduced polarization of the cells, mediated by a lack of MLC phosphorylation, resulting in radial F-actin and pseudopod formation. PMID: 24163421
    12. The tumor suppressor gene DAPK2 is induced by the myeloid transcription factors PU.1 and C/EBPalpha during granulocytic differentiation but repressed by PML-RARalpha in APL. PMID: 24038216
    13. DRP-1 and ZIPk most likely evolved from their ancient ancestor gene DAPk by two gene duplication events that occurred close to the emergence of vertebrates PMID: 21408167
    14. Sodium butyrate induced DAPK1/2 expression in human gastric cancer cells and this expression prompted apoptosis by decreasing FAK levels. PMID: 22160140
    15. High frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients PMID: 11839660
    16. distinct methylation pattern in bladder cancer with frequent methylation of RARbeta, DAPK, E-cadherin, and p16. PMID: 11839665
    17. gene expression in colorectal and gastric cancer silenced by DNA methylation and histone deacetylation PMID: 12087472
    18. Results implicate a novel role for DAPK2 in the regulation of normal myelopoiesis. PMID: 17347302
    19. DAPK2 as a novel Sp1-dependent target gene for E2F1 and KLF6 in cell death response. PMID: 18521079
    20. beta-catenin-induced down-regulation of DAPk-2 represents a novel signaling mechanism by which beta-catenin promotes the survival of malignant epithelial cells PMID: 18957423

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
  • 组织特异性：
    Expressed in neutrophils and eosinophils. Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
  • 数据库链接：

    HGNC: 2675

    OMIM: 616567

    KEGG: hsa:23604

    STRING: 9606.ENSP00000261891

    UniGene: Hs.237886